Modern Applications Of Dna Amplification Techniques
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Modern Applications Of Dna Amplification Techniques
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Author : Dirk Lassner
language : en
Publisher: Springer Science & Business Media
Release Date : 1997
Modern Applications Of Dna Amplification Techniques written by Dirk Lassner and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Medical categories.
The polymerase chain reaction (PCR) method of nucleic acid replication and modification now rivals traditional cloning, sequencing, and gene detecting procedures. Thirteen papers from the September 1996 meeting provide practical accounts of the authors' particular areas of PCR technology, and discus
Modern Applications Of Dna Amplification Techniques
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Author : Dirk Lassner
language : en
Publisher: Springer
Release Date : 2013-11-11
Modern Applications Of Dna Amplification Techniques written by Dirk Lassner and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
In the ten years since the first publication on PCR (Saiki et al. , 1985), this in vitro method of nucleic acid replication and modification has grown to rival in popularity traditional microbiological, genetical und technical procedures for cloning, sequencing, gene detecting and related procedures. To date the PCR literature has emphasized six main areas of application: genetic mapping, detection of mutations, genetic polymorphism, transcriptional splicing and regulation, molecular virology and quantitative procedures. The overwhelming focus of quantification of DNA or RNA by PCR has been on human microbiology and oncological problems. The exquisite sensitivity of PCR gives this method the ability to detect extremely rare DNAs, mRNAs, mRNAs in small numbers of cells or in small amounts of tissue, and mRNAs expressed in mixed-cell populations. However, the exact and accurate quantification of specific nucleic acids in biological samples is in spite of numerous publications in that field still a general problem: during the peR process, an unknown initial number of target sequences are used as a template from which a large quantity of specific product can be obtained. Although the amount of product formed is easy to determine, it is difficult to deduce the initial copy number of the target molecule because the efficiency of the peR is largely unknown.
Dna Amplification
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Author : Vadim V. Demidov
language : en
Publisher: Taylor & Francis
Release Date : 2004
Dna Amplification written by Vadim V. Demidov and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Science categories.
Whereas most books on DNA amplification focus on PCR-based technologies, this volume presents a wider range of methods to amplify DNA with an emphasis on their diverse applications. The book covers both well-established and newly-developed protocols including ligation-based thermocycling approaches, real-time PCR and other new PCR developments, plus several powerful non-PCR isothermal DNA amplification techniques, for example: real-time strand displacement amplification (SDA), rolling-circle amplification (RCA) and multiple-displacement amplification (MDA). An entire section is devoted to a group of enzymes, both natural and engineered, which are employed for DNA amplification and related purposes. In addition, the use of DNA amplification in the detection of non-DNA analytes is presented.
Pcr Technology
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Author : Henry A. Erlich
language : en
Publisher: Oxford University Press, USA
Release Date : 1992
Pcr Technology written by Henry A. Erlich and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with DNA categories.
Polymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for.
Methods In Dna Amplification
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Author : Ulrich Finckh
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Methods In Dna Amplification written by Ulrich Finckh and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
The polymerase chain reaction (PCR) - an in Vitro techniques for producing large amounts of a specific DNA fragment - has rapidly become established as one of the most important, impressive and fascinating methods of molecular biology as well as clinical diagnostics. In the seven years since'the technique was published, it has had a major impact on medical research. However, as there are still problems in instruments, standardized protocols for diagnostic applications and unsolved difficulties to avoid cross-contaminations on the one hand and on the other hand the even present question of how to interpret the biological value of a PCR result, most clinicians prefer to further wait until these topics are clarified. It is the aim of this book to give the reader lab-proven protocols from experienced scientists as well as a general introduction to alternative DNA-amplification procedures and their possible usage such as the NASBA or LCR. This book is divided into four major parts to provide a theoretical (first and second section) and a practical framework for a better understanding of the new technology. In the first part we provide an up-to-date summary of basic problems in this rapidly evolving field. We demonstrate, for example how to use fixed tissue materials and how to quantify PCR products as well as how to prepare nucleic acids in a safe, convenient and proper way, or even how to sequence directly PCR products for the analysis of the DNA structure.
Nucleic Acids Hybridization
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Author : Anton Buzdin
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-06-07
Nucleic Acids Hybridization written by Anton Buzdin and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-06-07 with Science categories.
Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and biomedicine, while the potential of many other advantageous techniques seems to be underestimated. Written by an international team of authors, this book details the current state-of-art in hybridization techniques. Coverage includes experimental protocols along with comprehensive and detailed method descriptions.
Transfusion Medicine And Patient Safety
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Author : Giustina De Silvestro
language : en
Publisher: Walter de Gruyter
Release Date : 2013-01-01
Transfusion Medicine And Patient Safety written by Giustina De Silvestro and has been published by Walter de Gruyter this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-01-01 with Medical categories.
Transfusional medicine is a young discipline, whereas the AB0 system was discovered only at the beginning of the last century, and that the neonatal haemolytic disease has been described in the middle of the 20th century. However, the evolution of technologies and diagnostic systems for immunohaematology, especially in recent decades, has been very intense and fast, and today the transfusional process, from the donation of blood until the transfusion of the patient, is perfectly controlled. This volume aims to highlight the main critical moments in which the operator must pay close attention, and at the same time offers the cultural, organizational and instrumental means for error prevention.
Ancient Dna Typing
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Author : Susanne Hummel
language : en
Publisher: Springer Science & Business Media
Release Date : 2003
Ancient Dna Typing written by Susanne Hummel and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Medical categories.
This is the definitive source of information on techniques for the identification and sequencing of old DNA (pieces) and their use in biological and medical research and application. Application of aDNA techniques are useful tools for investigations reaching from evolutionary studies to law enforcement approaches. What brings them together is the interest in specific methods of handling aDNA, i.e. elaborated PCR and sequencing techniques and the interpretation of the results. This books serves as an ideal guideline for it demonstrates how problem-solving strategies can be applied in various areas.
Modern Applications Of Plant Biotechnology In Pharmaceutical Sciences
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Author : Saurabh Bhatia
language : en
Publisher: Academic Press
Release Date : 2015-07-22
Modern Applications Of Plant Biotechnology In Pharmaceutical Sciences written by Saurabh Bhatia and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-07-22 with Science categories.
Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis Examines critical issues of international importance and offers real-life examples and potential solutions
Modern Techniques For Pathogen Detection
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Author : Jürgen Popp
language : de
Publisher: John Wiley & Sons
Release Date : 2015-06-08
Modern Techniques For Pathogen Detection written by Jürgen Popp and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-06-08 with Science categories.
This outstanding overview sets a new standard for a methods book on pathogen detection. The first chapter provides an outline of currently used routine methods, including their background, strengths and weaknesses, as well as comparing them to newer methods. The following chapters then cover novel methods already in wide use and which are still more experimental for routine purposes. An invaluable resource for all medical laboratories and clinical institutions dealing with infectious diseases.