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Chemical And Synthetic Biology Approaches To Understand Cellular Functions Part B

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Chemical And Synthetic Biology Approaches To Understand Cellular Functions Part B

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Author :
language : en
Publisher: Academic Press
Release Date : 2019-05-30

Chemical And Synthetic Biology Approaches To Understand Cellular Functions Part B written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-30 with Science categories.

Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Part B, Volume 622, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting chapters that cover the Design of optogenetic proteins, the Application of optogenetic proteins, Antibody aggregation mechanism probed by a fluorescently-labeled antibody with fluorescence correlation spectroscopy, Bimane labeling of B-arrestins to measure their interaction with GPCRs, Reversible biotinylation of proteins for investigating their interaction with partners, Chemical biology approaches to study RNA cytidine acetylation, Salt sensitive intein in robotic production of peptides, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on methods to measure ubiquitin chain length and linkage and genetic approaches to study the yeast ubiquitin system, amongst other timely topics

Chemical And Synthetic Biology Approaches To Understand Cellular Functions Part A

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Author :
language : en
Publisher: Academic Press
Release Date : 2019-05-23

Chemical And Synthetic Biology Approaches To Understand Cellular Functions Part A written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-23 with Science categories.

Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A, Volume 621, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this volume covering Site-directed ethylation of membrane proteins for measuring conformational transitions in lipid bilayers, the Design and synthesis of fluorescent activity probes for protein phosphatases, Stains, Utilizing split-nanoLuc fragments as luminescent probes for protein solubility in living cells, SH2-domain based sensor for intracellular recognition of sulfo-tyrosine, DNA-encoded immunoglobulins for detection of parasites, An engineered TEV protease – calmodulin fusion based sensor for neuronal calcium recording, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on methods to measure ubiquitin chain length and linkage and genetic approaches to study the yeast ubiquitin system, amongst other timely topics

Chemical And Synthetic Biology Approaches To Understand Cellular Functions Part C

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Author :
language : en
Publisher: Academic Press
Release Date : 2020-02-10

Chemical And Synthetic Biology Approaches To Understand Cellular Functions Part C written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-02-10 with Science categories.

Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C, Volume 633, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial. This release includes sections on Next generation probes for molecular imaging in cells, Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein, Converting avidin to bind ligands other than biotin, especially steroids, Chemoenzymatic Labeling Strategy, Engineered Siderophores, Small molecules to inhibit bacterial population behavior, NMR tube bioreactor, Small molecule controlled RAS activation system, Small molecule regulated Cas9, the Design and application of synthetic receptors, and much more. - Contains the authority of authors who are leaders in their field - Provides a comprehensive source on new methods and research in enzymology

Siderophore And Related Natural Products Biosynthesis

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Author :
language : en
Publisher: Elsevier
Release Date : 2024-08-20

Siderophore And Related Natural Products Biosynthesis written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-08-20 with Science categories.

Methods in Enzymology Volume 702 highlights new advances in the field, with this new volume presenting interesting topics on Siderophore and related natural products biosynthesis - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology - Updated release includes the latest information on Siderophore and related natural products biosynthesis

Cell Free Synthetic Biology

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Author : Jian Li
language : en
Publisher: Frontiers Media SA
Release Date : 2022-01-13

Cell Free Synthetic Biology written by Jian Li and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-13 with Science categories.

Metabolic Engineering

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Author : Sang Yup Lee
language : en
Publisher: John Wiley & Sons
Release Date : 2021-06-02

Metabolic Engineering written by Sang Yup Lee and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-06-02 with Science categories.

Learn more about foundational and advanced topics in metabolic engineering in this comprehensive resource edited by leaders in the field Metabolic Engineering: Concepts and Applications delivers a one-stop resource for readers seeking a complete description of the concepts, models, and applications of metabolic engineering. This guide offers practical insights into the metabolic engineering of major cell lines, including E. Coli, Bacillus and Yarrowia Lipolytica, and organisms, including human, animal, and plant). The distinguished editors also offer readers resources on microbiome engineering and the use of metabolic engineering in bioremediation. Written in two parts, Metabolic Engineering begins with the essential models and strategies of the field, like Flux Balance Analysis, Quantitative Flux Analysis, and Proteome Constrained Models. It also provides an overview of topics like Pathway Design, Metabolomics, and Genome Editing of Bacteria and Eukarya. The second part contains insightful descriptions of the practical applications of metabolic engineering, including specific examples that shed light on the topics within. In addition to subjects like the metabolic engineering of animals, humans, and plants, you’ll learn more about: Metabolic engineering concepts and a historical perspective on their development The different modes of analysis, including flux balance analysis and quantitative flux analysis An illuminating and complete discussion of the thermodynamics of metabolic pathways The Genome architecture of E. coli, as well as genome editing of both bacteria and eukarya An in-depth treatment of the application of metabolic engineering techniques to organisms including corynebacterial, bacillus, and pseudomonas, and more Perfect for students of biotechnology, bioengineers, and biotechnologists, Metabolic Engineering: Concepts and Applications also has a place on the bookshelves of research institutes, biotechnological institutes and industry labs, and university libraries. It's comprehensive treatment of all relevant metabolic engineering concepts, models, and applications will be of use to practicing biotechnologists and bioengineers who wish to solidify their understanding of the field.

Physico Chemical Control Of Cell Function

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Author : Cesare Gargioli
language : en
Publisher: Frontiers Media SA
Release Date : 2019-10-17

Physico Chemical Control Of Cell Function written by Cesare Gargioli and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-17 with categories.

Extracellular Matrix (ECM) has been considered for a long time merely a scaffold sustaining cell and tissue function. Despite this simplistic view shared by many, nowadays ECM and their mechanic-physical and chemical characteristic acquired a progressive larger role actively regulating cell life: survival, proliferation, gene expression and differentiation. The interplay between cells and the ECM is continuously controlled at the cell level in a dynamic way. While cells synthesize the raw components of the ECM, this in turn impacts on cell function by providing chemical, topographical and mechanical hints. Such stimuli have been proven to control several aspects of cell function, including survival, proliferation, differentiation and migration. The molecular pathways activated by cells in response to the physical cues arising from the ECM are being disclosed and thus the possibility to control cell function through materials design is becoming more realistic. Current in vitro protocols, relying in 2D cell culture system, entail reductionist approaches to the complexity of cell-ECM interaction and result in cells rapidly losing their distinctive functions in culture. Understanding and replicating the 3D microenvironmental cues affecting cell function appears as a mandatory requirement for the development of next-generation biomaterials, as well as for the establishment of more physiologically relevant and predictive in vitro models of diseases. Such an effort will require a multidisciplinary approach at the convergence of biophysics, biology, nanotechnology, and bioengineering.

Chemical And Synthetic Biology Approaches To Understand Cellular Functions Part B

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Author :
language : en
Publisher: Academic Press
Release Date : 2019-05-30

Synthetic Biology

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Author : Huimin Zhao
language : en
Publisher: Academic Press
Release Date : 2013-03-21

Synthetic Biology written by Huimin Zhao and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-21 with Science categories.

Synthetic Biology provides a framework to examine key enabling components in the emerging area of synthetic biology. Chapters contributed by leaders in the field address tools and methodologies developed for engineering biological systems at many levels, including molecular, pathway, network, whole cell, and multi-cell levels. The book highlights exciting practical applications of synthetic biology such as microbial production of biofuels and drugs, artificial cells, synthetic viruses, and artificial photosynthesis. The roles of computers and computational design are discussed, as well as future prospects in the field, including cell-free synthetic biology and engineering synthetic ecosystems.Synthetic biology is the design and construction of new biological entities, such as enzymes, genetic circuits, and cells, or the redesign of existing biological systems. It builds on the advances in molecular, cell, and systems biology and seeks to transform biology in the same way that synthesis transformed chemistry and integrated circuit design transformed computing. The element that distinguishes synthetic biology from traditional molecular and cellular biology is the focus on the design and construction of core components that can be modeled, understood, and tuned to meet specific performance criteria and the assembly of these smaller parts and devices into larger integrated systems that solve specific biotechnology problems. - Includes contributions from leaders in the field presents examples of ambitious synthetic biology efforts including creation of artificial cells from scratch, cell-free synthesis of chemicals, fuels, and proteins, engineering of artificial photosynthesis for biofuels production, and creation of unnatural living organisms - Describes the latest state-of-the-art tools developed for low-cost synthesis of ever-increasing sizes of DNA and efficient modification of proteins, pathways, and genomes - Highlights key technologies for analyzing biological systems at the genomic, proteomic, and metabolomic levels which are especially valuable in pathway, whole cell, and multi-cell applications - Details mathematical modeling tools and computational tools which can dramatically increase the speed of the design process as well as reduce the cost of development

When Chemistry Meets Biology Generating Innovative Concepts Methods And Tools For Scientific Discovery In The Plant Sciences

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Author : Erich Kombrink
language : en
Publisher: Frontiers Media SA
Release Date : 2016-08-12

When Chemistry Meets Biology Generating Innovative Concepts Methods And Tools For Scientific Discovery In The Plant Sciences written by Erich Kombrink and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-12 with Botany categories.

Biologically active small molecules have increasingly been applied in plant biology to dissect and understand biological systems. This is evident from the frequent use of potent and selective inhibitors of enzymes or other biological processes such as transcription, translation, or protein degradation. In contrast to animal systems, which are nurtured from drug research, the systematic development of novel bioactive small molecules as research tools for plant systems is a largely underexplored research area. This is surprising since bioactive small molecules bear great potential for generating new, powerful tools for dissecting diverse biological processes. In particular, when small molecules are integrated into genetic strategies (thereby defining “chemical genetics”), they may help to circumvent inherent problems of classical (forward) genetics. There are now clear examples of important, fundamental discoveries originating from plant chemical genetics that demonstrate the power, but not yet fully exploited potential, of this experimental approach. These include the unraveling of molecular mechanisms and critical steps in hormone signaling, activation of defense reactions and dynamic intracellular processes. The intention of this Research Topic of Frontiers in Plant Physiology is to summarize the current status of research at the interface between chemistry and biology and to identify future research challenges. The research topic covers diverse aspects of plant chemical biology, including the identification of bioactive small molecules through screening processes from chemical libraries and natural sources, which rely on robust and quantitative high-throughput bioassays, the critical evaluation and characterization of the compound’s activity (selectivity) and, ultimately, the identification of its protein target(s) and mode-of-action, which is yet the biggest challenge of all. Such well-characterized, selective chemicals are attractive tools for basic research, allowing the functional dissection of plant signaling processes, or for applied purposes, if designed for protection of crop plants from disease. New methods and data mining tools for assessing the bioactivity profile of compounds, exploring the chemical space for structure–function relationships, and comprehensive chemical fingerprinting (metabolomics) are also important strategies in plant chemical biology. In addition, there is a continuing need for diverse target-specific bioprobes that help profiling enzymatic activities or selectively label protein complexes or cellular compartments. To achieve these goals and to add suitable probes and methods to the experimental toolbox, plant biologists need to closely cooperate with synthetic chemists. The development of such tailored chemicals that beyond application in basic research can modify traits of crop plants or target specific classes of weeds or pests by collaboration of applied and academic research groups may provide a bright future for plant chemical biology. The current Research Topic covers the breadth of the field by presenting original research articles, methods papers, reviews, perspectives and opinions.

Chemical And Synthetic Biology Approaches To Understand Cellular Functions Part B

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