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Fluorescence In Situ Hybridization Fish Application Guide


Fluorescence In Situ Hybridization Fish Application Guide
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Fluorescence In Situ Hybridization Fish Application Guide


Fluorescence In Situ Hybridization Fish Application Guide
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Author : Thomas Liehr
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-11-26

Fluorescence In Situ Hybridization Fish Application Guide written by Thomas Liehr and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-26 with Science categories.


This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.



Fluorescence In Situ Hybridization Fish Application Guide


Fluorescence In Situ Hybridization Fish Application Guide
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Author : Thomas Liehr
language : en
Publisher: Springer
Release Date : 2008-11-18

Fluorescence In Situ Hybridization Fish Application Guide written by Thomas Liehr and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-18 with Science categories.


This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.



Genetics Evolution And Conservation Of Neotropical Fishes


Genetics Evolution And Conservation Of Neotropical Fishes
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Author : Rodrigo A. Torres
language : en
Publisher: Frontiers Media SA
Release Date : 2020-01-21

Genetics Evolution And Conservation Of Neotropical Fishes written by Rodrigo A. Torres and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-01-21 with categories.


Fish represent the most ancestral and specious group of vertebrates, and occupy more diverse aquatic environments around the world. Ichthyofauna is extremely diverse, especially in megadiverse countries occupying biogeographical regions such as the Neotropical Region, which covers an extensive area between North and South America. Much of this biodiversity will be extinct, even before science knows any aspect of its biology. Like this, Neotropical fish genetics started in the end of the 70’s with papers studying the chromosomes of Hoplias malabaricus (Family Erythrinidae) and the karyotype variation among three genera of the family Anostomidae. The topic at that time was concentrated in two Institutions from the state of São Paulo, Southeastern Brazil. In the middle 80’s, the first Symposium on Neotropical Fish Cytogenetics was organized. Nowadays, the field of Neotropical Fish Genetics is present in Brazil, Colombia, Argentina, Uruguai, Venezula, Chile, and Equador, as well as outside South America in Panama, Mexico, USA, Canada, Czech Republic, Germany, and Spain. The research developed in cytogenetics has focused mainly on karyotype evolution and cytotaxonomy, chromosome structure and, more recently, cytogenomics. In relation to the use of molecular markers, support has been sought for the management of populations for conservation or production in captivity. In addition, many studies have been carried out with the aim of establishing supra-specific phylogenetic relationships and clarifying species distribution scenarios by phylogeographic modeling. The genome and transcriptome of some model species begin to emerge as extremely promising and informative areas for neotropical fish. In 2017, the Neotropical fish genetics research community celebrates the 30th anniversary of its main Meeting (today entitled Symposium on Neotropical Fish Genetics and Cytogenetics). This Research Topic is part of this celebration and aims at reporting the state of the art and its current advances in the frontier of knowledge in genetics, evolution, and conservation of neotropical fish, as well as to detect the challenges to be overcome in the next years.



Handbook Of Laser Technology And Applications


Handbook Of Laser Technology And Applications
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Author : Colin Webb
language : en
Publisher: CRC Press
Release Date : 2020-09-29

Handbook Of Laser Technology And Applications written by Colin Webb and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-09-29 with Mathematics categories.


The invention of the laser was one of the towering achievements of the twentieth century. At the opening of the twenty-first century we are witnessing the burgeoning of the myriad technical innovations to which that invention has led. The Handbook of Laser Technology and Applications is a practical and long-lasting reference source for scientists and engineers who work with lasers. The Handbook provides, a comprehensive guide to the current status of lasers and laser systems; it is accessible to science or engineering graduates needing no more than standard undergraduate knowledge of optics. Whilst being a self-contained reference work, the Handbook provides extensive references to contemporary work, and is a basis for studying the professional journal literature on the subject. It covers applications through detailed case studies, and is therefore well suited to readers who wish to use it to solve specific problems of their own. The first of the three volumes comprises an introduction to the basic scientific principles of lasers, laser beams and non-linear optics. The second volume describes the mechanisms and operating characteristics of specific types of laser including crystalline solid - state lasers, semiconductor diode lasers, fibre lasers, gas lasers, chemical lasers, dye lasers and many others as well as detailing the optical and electronic components which tailor the laser's performance and beam delivery systems. The third volume is devoted to case studies of applications in a wide range of subjects including materials processing, optical measurement techniques, medicine, telecommunications, data storage, spectroscopy, earth sciences and astronomy, and plasma fusion research. This vast compendium of knowledge on laser science and technology is the work of over 130 international experts, many of whom are recognised as the world leaders in their respective fields. Whether the reader is engaged in the science, technology, industrial or medical applications of lasers or is researching the subject as a manager or investor in technical enterprises they cannot fail to be informed and enlightened by the wide range of information the Handbook supplies.



Cytogenetics


Cytogenetics
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Author : Marcelo L. Larramendy
language : en
Publisher: BoD – Books on Demand
Release Date : 2019-05-10

Cytogenetics written by Marcelo L. Larramendy and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-10 with Medical categories.


Cytogenetics - Past, Present, and Further Perspectives discusses events that influenced the development of cytogenetics as a specialty within biology, with special attention paid to methodological achievements developed worldwide that have driven the field forward. Improvements to the resolution of chromosome analysis followed closely the introduction of innovative analytical technologies. In that sense, this book reviews and provides a brief account of the structure of chromosomes and stresses the high structural conservation in different species with an emphasis on aspects that require further research. However, it should be kept in mind that the future of cytogenetics will likely depend on improved knowledge of chromosome structure and function.



Human Interphase Chromosomes


Human Interphase Chromosomes
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Author : Ivan Iourov
language : en
Publisher: Springer Nature
Release Date : 2021-01-29

Human Interphase Chromosomes written by Ivan Iourov and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-01-29 with Medical categories.


This second edition focuses on the study of human interphase chromosomes and its relation to health and disease. Orchestrated organization and behavior of the human genome in interphase nuclei at chromosomal level has been repeatedly shown to play a significant role in almost all basic biological processes involved in the processing and inheritance of genetic information within and between species. Accordingly, post-genomic bioscience appeals to basic and applied studies of interphase nuclei genetics and genomics with special attention to interphase chromosome behavior in health and disease. Additionally, elucidating the role of interphase chromosome behavior during development, chromosome/DNA replication, DNA reparation opens new horizons for basic and applied bioscience Studies of interphase nuclei have an appreciable impact on different areas of biomedical sciences such as cell biology, neurobiology, cancer research, developmental biology, epigenetics, cytogenetics, and medical genetics, as a whole. Moreover, development of innovative and emergent technologies to analyze interphase nuclei are closely associated with application of these techniques in diagnostic and research practices to solve reproductive problems (including infertility and spontaneous abortions), to investigate congenital malformations (including those produced by aneuploidy and other chromosome abnormalities); genetic diseases (including cardiac, immune, neurological and psychiatric diseases), and cancer. This second edition serves as a source of updated valuable information and promising ideas for a wide audience of professionals in biomedicine including researchers, scientists, and healthcare professionals in human genetics, cytogenetics, and developmental biology.



The Agt Cytogenetics Laboratory Manual


The Agt Cytogenetics Laboratory Manual
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Author : Marilyn S. Arsham
language : en
Publisher: John Wiley & Sons
Release Date : 2017-04-24

The Agt Cytogenetics Laboratory Manual written by Marilyn S. Arsham and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-04-24 with Science categories.


Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.



Handbook Of Animal Based Fermented Food And Beverage Technology


Handbook Of Animal Based Fermented Food And Beverage Technology
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Author : Y. H. Hui
language : en
Publisher: CRC Press
Release Date : 2016-04-19

Handbook Of Animal Based Fermented Food And Beverage Technology written by Y. H. Hui and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-19 with Technology & Engineering categories.


Fermented food can be produced with inexpensive ingredients and simple techniques and makes a significant contribution to the human diet, especially in rural households and village communities worldwide. Progress in the biological and microbiological sciences involved in the manufacture of these foods has led to commercialization and heightened int



Prospects And Applications For Plant Associated Microbes A Laboratory Manual


Prospects And Applications For Plant Associated Microbes A Laboratory Manual
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Author : Seppo Sorvari
language : en
Publisher: Seppo Sorvari
Release Date : 2014-12-15

Prospects And Applications For Plant Associated Microbes A Laboratory Manual written by Seppo Sorvari and has been published by Seppo Sorvari this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-12-15 with Science categories.


Research on the microbial colonization of the aerial and subterranean tissues of plants has shown an extensive scale of interactions between the hosts and a range of microbes, including bacteria and fungi. Intercellular spaces, vascular systems and even single cells can be inhabited by these endophytic microbes. Of the bacterial endophytes, only a small percentage is harmful to the plant; most are neutral, opportunistic or beneficial. These plant-based bacteria can have various important functions throughout the life cycle of the plant; some promote plant growth and development, others protect the plant from diseases. This ability to be able to protect plants from diseases has catalyzed numerous laboratories to search for new bacteria that could be utilized instead of the traditional plant-protective agents. Because two or more interacting organisms are involved, research and the eventual application of suitable bio-controlling microbes are challenging and often require specific skills and equipment. The purpose of this book is to provide a comprehensive review for those who are interested in the research and biotechnological applications of plant-associated bacteria. It also provides a compilation of current work conducted on plant-bacteria interactions.



Handbook Of Animal Based Fermented Food And Beverage Technology Second Edition


Handbook Of Animal Based Fermented Food And Beverage Technology Second Edition
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Author : Y. H. Hui
language : en
Publisher: CRC Press
Release Date : 2012-05-14

Handbook Of Animal Based Fermented Food And Beverage Technology Second Edition written by Y. H. Hui and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-05-14 with Technology & Engineering categories.


Fermented food can be produced with inexpensive ingredients and simple techniques and makes a significant contribution to the human diet, especially in rural households and village communities worldwide. Progress in the biological and microbiological sciences involved in the manufacture of these foods has led to commercialization and heightened interest among scientists and food processors. Handbook of Animal-Based Fermented Food and Beverage Technology, Second Edition is an up-to-date reference exploring the history, microorganisms, quality assurance, and manufacture of fermented food products derived from animal sources. The book begins by describing fermented animal product manufacturing and then supplies a detailed exploration of a range of topics including: Dairy starter cultures, microorganisms, leuconostoc and its use in dairy technology, and the production of biopreservatives Exopolysaccharides and fermentation ecosystems Fermented milk, koumiss, laban, yogurt, and sour cream Meat products, including ham, salami, sausages, and Turkish pastirma Malaysian and Indonesian fermented fish products Probiotics and fermented products, including the technological aspects and benefits of cheese as a probiotic carrier Fermented food products play a critical role in cultural identity, local economy, and gastronomical delight. With contributions from over 60 experts from more than 20 countries, the book is an essential reference distilling the most critical information on this food sector.