Immobilized Ph Gradients Theory And Methodology
DOWNLOAD eBooks
Download Immobilized Ph Gradients Theory And Methodology PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Immobilized Ph Gradients Theory And Methodology book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Immobilized Ph Gradients Theory And Methodology
DOWNLOAD eBooks
Author : Pier Giorgio Righetti
language : en
Publisher: Elsevier
Release Date : 1990-02-07
Immobilized Ph Gradients Theory And Methodology written by Pier Giorgio Righetti and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-02-07 with Science categories.
Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, protein precipitation at the pI), it turned out to be an entirely new technique, quite different in principle and operation from conventional IEF. The book is thus meant to bring the reader up to date with this fast developing field. The book is divided into six chapters containing information on: detailed treatment of all the chemistry of the Immobiline chemicals; theory of pH gradient generation (computer simulations, tables with all the possible pH recipes); all analytical aspects of IPGs, including staining techniques, blotting etc.; two-dimensional maps, with a detailed treatise of advantages and limitations; preparative aspects of IPGs, including comparison with other preparative electrophoretic techniques; some examples of applications, including genetic and forensic analysis, blood polymorphism etc. The book is extensive and up-to-date, while also extensively covering the theory. Clearly written, with easily accessible information, the volume is a worthy asset to the Lab. Techniques series.
Immobilized Ph Gradients Theory And Methodology
DOWNLOAD eBooks
Author : P. G. Righetti
language : en
Publisher: Elsevier
Release Date : 1990
Immobilized Ph Gradients Theory And Methodology written by P. G. Righetti and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Science categories.
Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, protein precipitation at the pI), it turned out to be an entirely new technique, quite different in principle and operation from conventional IEF. The book is thus meant to bring the reader up to date with this fast developing field. The book is divided into six chapters containing information on: detailed treatment of all the chemistry of the Immobiline chemicals; theory of pH gradient generation (computer simulations, tables with all the possible pH recipes); all analytical aspects of IPGs, including staining techniques, blotting etc.; two-dimensional maps, with a detailed treatise of advantages and limitations; preparative aspects of IPGs, including comparison with other preparative electrophoretic techniques; some examples of applications, including genetic and forensic analysis, blood polymorphism etc.The book is extensive and up-to-date, while also extensively covering the theory. Clearly written, with easily accessible information, the volume is a worthy asset to the Lab. Techniques series.
Handbook Of Isoelectric Focusing And Proteomics
DOWNLOAD eBooks
Author : David Garfin
language : en
Publisher: Academic Press
Release Date : 2005-05-09
Handbook Of Isoelectric Focusing And Proteomics written by David Garfin and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-05-09 with Science categories.
Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics. * Is the only current book available on the subject * Includes author insights and practical tips * Is an ideal single source for students and researchers working in proteomics
The Protein Protocols Handbook
DOWNLOAD eBooks
Author : John M. Walker
language : en
Publisher: Springer
Release Date : 2007-10-02
The Protein Protocols Handbook written by John M. Walker and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-02 with Science categories.
The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Theoretical Advancement In Chromatography And Related Separation Techniques
DOWNLOAD eBooks
Author : Francesco Dondi
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Theoretical Advancement In Chromatography And Related Separation Techniques written by Francesco Dondi and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Chromatography and all the related separation techniques are experimental in their origin and justification. However, the spectacular progress made in this area since World War II has given rise to a theoretical underpinning. The present book covers the current status of the research area and places it in perspective with the general concepts of the fields of physical chemistry involved. The ASI lectures/authors -- well known leaders in their fields -- have written presentations at the graduate level, accessible to all those who have a good general background in the thermodynamics and mass transfer theory of phase equilibria. The book will be useful to young scientists and engineers who wish to access the current frontiers in chromatography and other separation sciences.
Separation Methods In Proteomics
DOWNLOAD eBooks
Author : Gary B. Smejkal
language : en
Publisher: CRC Press
Release Date : 2005-12-12
Separation Methods In Proteomics written by Gary B. Smejkal and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-12-12 with Science categories.
Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomics research. Written as a compilation of hands-on methods exemplified by the work of several recognized leaders in the field, this book may serve as a guide for selection of the optimal separation strategies to solve particular biological problems. Recent progress in the development of robust analytical techniques and instrumentation has created the need for good quality biological samples that are subject to analysis. Emphasizing the importance of sample preparation, the book explains how proteomes can be divided into smaller, less complicated “subproteomes" for individual analysis. It also highlights several hybrid approaches that take into account protein interactions. Including applications of the separation methods currently employed in proteomic analyses for both clinical and basic research, Separation Methods in Proteomics contains practical information that can enhance the current and future endeavors of scientists in proteomics, genomics, transcriptomics, biomarker discovery, and drug discovery.
Biochemistry And Molecular Biology Compendium
DOWNLOAD eBooks
Author : Roger L. Lundblad
language : en
Publisher: CRC Press
Release Date : 2019-11-11
Biochemistry And Molecular Biology Compendium written by Roger L. Lundblad and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-11-11 with Medical categories.
This book is an accessible resource offering practical information not found in more database-oriented resources. The first chapter lists acronyms with definitions, and a glossary of terms and subjects used in biochemistry, molecular biology, biotechnology, proteomics, genomics, and systems biology. There follows chapters on chemicals employed in biochemistry and molecular biology, complete with properties and structure drawings. Researchers will find this book to be a valuable tool that will save them time, as well as provide essential links to the roots of their science. Key selling features: Contains an extensive list of commonly used acronyms with definitions Offers a highly readable glossary for systems and techniques Provides comprehensive information for the validation of biotechnology assays and manufacturing processes Includes a list of Log P values, water solubility, and molecular weight for selected chemicals Gives a detailed listing of protease inhibitors and cocktails, as well as a list of buffers
Proteome Analysis
DOWNLOAD eBooks
Author : David W Speicher
language : en
Publisher: Elsevier
Release Date : 2004-03-26
Proteome Analysis written by David W Speicher and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-03-26 with Science categories.
State-of-the-art protein profiling methods include high resolution two-dimensional gels, two-dimensional differential in-gel electrophoresis, LC-MS and LC-MS/MS using accurate mass tags, and protein identifications of proteins from gels using mass spectrometry methods are discussed in depth. Other chapters describe comprehensive characterization of proteomes using electrophoretic prefractionation and analyses of sub-proteomes based on specific posttranslational modifications including the phospho-proteome, the glyco-proteome, and nitrated proteins. These conventional proteome analysis chapters are complemented by discussion of emerging technologies and approaches such as affinity based biosensor proteomics as well as the use of protein microarrays, microfluidics and nanotechnology. Strategies for improving throughput by automation are also discussed.-
2 D Proteome Analysis Protocols
DOWNLOAD eBooks
Author : Andrew J. Link
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
2 D Proteome Analysis Protocols written by Andrew J. Link and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry—especially in the fields of molecular biology, biochemistry, microbiology, and cell biology—should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.
Proteomics In Functional Genomics
DOWNLOAD eBooks
Author : P. Jolles
language : en
Publisher: Birkhäuser
Release Date : 2013-03-11
Proteomics In Functional Genomics written by P. Jolles and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-11 with Science categories.
A wealth of information has accumulated over the last few years on the human genome. The new insights have completely changed the focus of protein analysis. It is no longer time-consuming analysis of unknown products, but rather selective identifications of individual forms, modifications and processings, and overall analysis of global protein outputs from cells and tissues in health and disease. This book gears to the rising need of sensitive, accurate, and fast separation and identification techniques in proteomics. It discusses current methodologies of modern protein analysis, from isolation and sample preparation, over analysis and identification, to final characterization. Several evaluations concentrate on the now productive approaches of two-dimensional gel electrophoresis and mass spectrometry, but alternative methods and further perspectives are also outlined. The book includes an overlook over current databases to connect protein analysis data with all available information,...