Proximity Labeling
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Proximity Labeling
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Author : Murat Sunbul
language : en
Publisher: Springer Nature
Release Date : 2019-05-23
Proximity Labeling written by Murat Sunbul and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-23 with Science categories.
This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.
Proximity Dependent Protein Biotinylation
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Author : Petra Van Damme
language : en
Publisher: Springer Nature
Release Date : 2025-07-10
Proximity Dependent Protein Biotinylation written by Petra Van Damme and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-07-10 with Science categories.
This volume explores the cutting-edge of proximity-dependent protein biotinylation approaches, focusing on innovative biotin ligase (BioID) and peroxidase-based methods, like APEX. Spanning a diverse array of biological systems, including bacteria, zebrafish, plants, and mammals, this collection showcases methods that aid in the identification of protein interactions, localization, membrane topology, trafficking, and secretion dynamics. Additionally, the book features applications at the host/microbe interface and for the use of tissue-specific biotinylation for studying interorgan communication, as well as in silico validation methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity-Dependent Protein Biotinylation: Methods and Protocols serves as an essential guide for researchers advancing the study of proxeome biology.
Methods For Plant Nucleus And Chromatin Studies
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Author : Célia Baroux
language : en
Publisher: Springer Nature
Release Date : 2024-11-22
Methods For Plant Nucleus And Chromatin Studies written by Célia Baroux and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-11-22 with Science categories.
This volume provides a comprehensive collection of protocols that can be used to study plant chromatin structure and composition. Chapters divided into five sections detail the profiling of chromatin features in relation to epigenetic regulation, investigate the interaction between chromatin modifications and gene regulation, and explore the 3D spatial organization of the chromatin inside the nucleus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Methods for Plant Nucleus and Chromatin Studies: Methods and Protocols aims to ensure successful results in the further study of this vital field.
The Evolving Chromatin And Transcriptional Landscapes Emerging Methods Tools And Techniques
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Author : Jean-Philippe Lambert
language : en
Publisher: Frontiers Media SA
Release Date : 2022-01-03
The Evolving Chromatin And Transcriptional Landscapes Emerging Methods Tools And Techniques written by Jean-Philippe Lambert and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-03 with Science categories.
All You Need To Know About Spatial Omics
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Author : HEBooks
language : en
Publisher: Hebooks
Release Date :
All You Need To Know About Spatial Omics written by HEBooks and has been published by Hebooks this book supported file pdf, txt, epub, kindle and other format this book has been release on with Antiques & Collectibles categories.
"All You Need to Know About Spatial Omics: Decoding Biology's Native Settings" is a comprehensive guide that unravels the complexities of biological landscapes through cutting-edge molecular technologies. This book provides an in-depth exploration of spatial omics, a revolutionary field that captures the intricate spatial organization of genes, proteins, metabolites, and cells within tissues, ecosystems, and organisms. From foundational concepts to advanced applications, readers will gain a thorough understanding of how spatial omics is transforming our understanding of health, disease, ecology, and environmental science. The book delves into key topics such as computational tools, ethical considerations, emerging trends, and real-world case studies, offering practical insights for researchers, clinicians, ecologists, and policymakers. With a focus on accessibility, innovation, and societal impact, this resource equips readers with the knowledge needed to harness the power of spatial omics for groundbreaking discoveries. Whether you're a seasoned scientist or new to the field, this book serves as an essential roadmap for decoding biology’s native settings and unlocking the potential of spatial omics to address some of the most pressing challenges facing humanity today.
Characterizing Biotherapeutics
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Author : Jennie R. Lill
language : en
Publisher: John Wiley & Sons
Release Date : 2025-06-04
Characterizing Biotherapeutics written by Jennie R. Lill and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-06-04 with Science categories.
Provides detailed and up-to-date coverage of analytical approaches for biologic drug modalities The development of innovative biologic therapies has revolutionized medicine, but their complexity demands equally advanced analytical approaches. Characterizing Biotherapeutics: Analytical Methods for Diverse Modalities introduces the tools and techniques used to analyze these groundbreaking therapies. Designed to help readers characterize and troubleshoot increasingly diverse and sophisticated therapeutic molecules, this in-depth guide addresses different biologic drugs, their unique analytical challenges, and the methods that enable their analysis. Organized into two comprehensive sections, Characterizing Biotherapeutics first delves into the fundamentals of analytical platforms, providing a robust foundation in techniques such as mass spectrometry and biophysical assays. The second section applies these methods to real-world scenarios, focusing on drug discovery, clinical evaluation, and commercial considerations in drug development. Authors Jennie R. Lill and Wendy Sandoval provide clear guidance tailored to the evolving demands of therapeutic innovations such as structural characterization, high-throughput biophysical assays, and RNA-based therapeutics. Equipping researchers with the knowledge to navigate the challenges posed by increasingly complex biologics, Characterizing Biotherapeutics: Analytical Methods for Diverse Modalities: Discusses new and emerging biological molecule diversity and both traditional analytical methods Provides characterization strategies for large molecule (protein/antibody) based therapeutics Summarizes cutting-edge technologies for novel modalities and antibody protein therapeutics Incorporates the latest advancements in mass spectrometry, functional assays, and biophysical methods Addresses specialized analytical issues for membrane proteins and other challenging targets Presents methodologies for assessing safety and therapeutic potential Characterizing Biotherapeutics: Analytical Methods for Diverse Modalities is an essential reference for analytical scientists, biologists, and mass spectrometrists involved in biomolecule analysis. It is also a valuable resource for graduate students taking advanced courses in biotechnology, drug development, and biotherapeutic analysis, as well as professionals in biotechnology and pharmaceutical industries working to advance biotherapeutic research and development.
Neuroproteomics
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Author : Angus C. Nairn
language : en
Publisher: MDPI
Release Date : 2020-02-12
Neuroproteomics written by Angus C. Nairn and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-02-12 with Science categories.
The Neuroproteomics Special Issue overviews the unique challenges that must be addressed to carry out meaningful MS/proteomics analyses on neural tissues and the technologies that are available to meet these challenges. The articles on Alzheimer’s disease, addiction, and schizophrenia illustrate how MS/proteomics technologies can be used to improve our ability to diagnose and understand the molecular basis for neurological diseases. Several articles will be of interest to investigators beyond the field of neurological disorders. The review on the discovery of biofluid biomarkers of neurodegenerative dementias will be of interest to investigators searching for other disease biomarkers. Similarly, the review on the role of neuroproteomics in elucidating mechanisms of drug addiction provides an overview of the utility of MS/proteomics approaches for addressing critical questions in addiction neuroscience that should be applicable to investigators involved in virtually any area of biomedical research. Likewise, the article on developing targeted MS approaches for quantifying postsynaptic density proteins will be useful for any investigator who wishes to design targeted assays for virtually any protein. Finally, the peroxidase-mediated proximity labeling technology, described in the article on mapping the proteome of the synaptic cleft, will be of interest to investigators interested in mapping other spatially restricted proteomes.
Guide To Research Techniques In Neuroscience
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Author : Matt Carter
language : en
Publisher: Academic Press
Release Date : 2022-03-26
Guide To Research Techniques In Neuroscience written by Matt Carter and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-03-26 with Medical categories.
Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. - Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods - Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more - Clear, straightforward explanations of each technique for anyone new to the field - A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture - Detailed recommendations on where to find protocols and other resources for specific techniques - "Walk-through" boxes that guide readers through experiments step-by-step
Biology Of Mycobacterial Lipids
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Author : Zeeshan Fatima
language : en
Publisher: Academic Press
Release Date : 2022-06-03
Biology Of Mycobacterial Lipids written by Zeeshan Fatima and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-06-03 with Science categories.
Biology of Mycobacterial Lipids covers various topics pertaining to the advancements and current research in the field of mycobacterial lipids, and on the significant progress in lipidomics, in recent times. The chapters present comprehensive, yet systematic cutting-edge research, comprising mycobacterial lipid terminologies, classifications, biosynthetic pathways, tools and techniques, and functional burgeoning. This unique reference book has contributions from pioneer researchers, experts, and eminent veterans from around the globe. It covers ground-breaking work that will bridge the gap between understanding biochemical patterns related to virulence, pathogenesis, and resistance and elucidating new targets for drug design, identifying biomarkers for predicting risk, early diagnosis, and therapeutic outcome. - Covers major biochemical aspects of mycobacterial lipids, nomenclature, structure and classification, and metabolic pathways - Focuses on recent trends and state-of-the-art technology, used in mycobacterial lipids study - Brings together the functional aspects of mycobacterial lipidome, involvement of lipids in cellular network and signaling, its involvement in virulence and resistance, and host factor manipulations - Highlights the discovery of lipid biomarkers, for diagnostic and therapeutic interventions, using mycobacterial lipidomic studies - Presents lipids at the interface of various other biomolecules with integrative omics aspects
High Throughput Screening For Drug Discovery
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Author : Shailendra K. Saxena
language : en
Publisher: BoD – Books on Demand
Release Date : 2022-05-25
High Throughput Screening For Drug Discovery written by Shailendra K. Saxena and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-05-25 with Medical categories.
The book focuses on various aspects and properties of high-throughput screening (HTS), which is of great importance in the development of novel drugs to treat communicable and non-communicable diseases. Chapters in this volume discuss HTS methodologies, resources, and technologies and highlight the significance of HTS in personalized and precision medicine.