Flow Cytometry Advd Res Clinical Applns
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Clinical Applications Of Flow Cytometry
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Author : G. E. Marti
language : en
Publisher:
Release Date : 1988
Clinical Applications Of Flow Cytometry written by G. E. Marti and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Flow cytometry categories.
Application Of Flow Cytometry In Clinical Cell Diagnostics
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Author :
language : en
Publisher:
Release Date : 1997
Application Of Flow Cytometry In Clinical Cell Diagnostics written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with categories.
Flow Cytometry In Dermatological Research And Clinical Practice
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Author : Julia Ann Newton
language : en
Publisher:
Release Date : 1986
Flow Cytometry In Dermatological Research And Clinical Practice written by Julia Ann Newton and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986 with categories.
New Applications Of Flow Cytometry
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Author : J. Philip McCoy
language : en
Publisher:
Release Date : 2001
New Applications Of Flow Cytometry written by J. Philip McCoy and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Flow cytometry categories.
Special Issue Advanced Flow Cytometry
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Author : Ranbir Chander Sobti
language : en
Publisher:
Release Date : 2002
Special Issue Advanced Flow Cytometry written by Ranbir Chander Sobti and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with categories.
Study Of Morphology Based Cell Assay By Diffraction Imaging Flow Cytometry
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Author : Wenhuan Jiang
language : en
Publisher:
Release Date : 2015
Study Of Morphology Based Cell Assay By Diffraction Imaging Flow Cytometry written by Wenhuan Jiang and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with Cytology categories.
Development of an accurate and label-free method for single cell assay attracts intensive research efforts for its importance to cell biology research and clinical applications. Flow cytometry is one of the most widely used technologies for rapid assay of single cells but existing approaches provide very limited information on cell morphology and require the fluorescence staining. In this dissertation research, we focus our efforts on the quantitative analysis of cell morphology using confocal microscopy based three-dimensional (3D) reconstruction and the exploration of a new approach of flow cytometry through imaging of highly coherent scattered light. The goal of the dissertation research is to develop a new and morphology-based approach for rapid cell assay and phenotyping with the polarization diffraction imaging flow cytometry (p-DIFC) platform through investigation of the structure-function relations at the cell level. To achieve this goal, cross-polarized diffraction image pairs have been acquired from single cells excited by a linearly polarized laser beam. Image texture and intensity parameters are extracted with a gray level co-occurrence matrix (GLCM) algorithm to obtain a set of image parameters to quantify the diffraction patterns. An automated cell classification method has been developed using a Support Vector Machine (SVM) algorithm in the feature space formed by the training data of the cross-polarized diffraction image pairs. We have investigated different types of human lymphocytes and prostate epithelial cells with the confocal imaging and p-DIFC measurements and conducted cell morphology and classification studies. The analysis of 3D morphology parameters among the six types of cells provides, for the first time, the ability to quantitatively evaluate the morphologic differences among these phenotypes and to gain insights on the morphology-based classification. It has been further shown that the diffraction image parameters can be mapped into a high-dimensional feature space with the SVM algorithm to obtain the optimized model and yield accurate classifications between Jurkat T cells and Ramos B cells and between the normal and cancerous prostate epithelial cells. Based on these results we conclude that the p-DIFC method has significant potentials to be developed into a rapid and label-free method for cell assay and morphology-based classification to discriminate cells of high similarity in their morphology.
Practical Flow Cytometry
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Author : Howard M. Shapiro
language : en
Publisher: John Wiley & Sons
Release Date : 2005-02-25
Practical Flow Cytometry written by Howard M. Shapiro and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-02-25 with Science categories.
From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.
Imaging Flow Cytometry
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Author : Natasha S. Barteneva
language : en
Publisher: Humana
Release Date : 2015-11-23
Imaging Flow Cytometry written by Natasha S. Barteneva and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-11-23 with Medical categories.
This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.
Innovations For Next Generation Antibody Drug Conjugates
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Author : Marc Damelin
language : en
Publisher: Springer
Release Date : 2018-05-29
Innovations For Next Generation Antibody Drug Conjugates written by Marc Damelin and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-29 with Medical categories.
Antibody-drug conjugates (ADCs) stand at the verge of a transformation. Scores of clinical programs have yielded only a few regulatory approvals, but a wave of technological innovation now empowers us to overcome past technical challenges. This volume focuses on the next generation of ADCs and the innovations that will enable them. The book inspires the future by integrating the field’s history with novel strategies and cutting-edge technologies. While the book primarily addresses ADCs for solid tumors, the last chapter explores the emerging interest in using ADCs to treat other diseases. The therapeutic rationale of ADCs is strong: to direct small molecules to the desired site of action (and away from normal tissues) by conjugation to antibodies or other targeting moieties. However, the combination of small and large molecules imposes deep complexity to lead optimization, pharmacokinetics, toxicology, analytics and manufacturing. The field has made significant advances in all of these areas by improving target selection, ADC design, manufacturing methods and clinical strategies. These innovations will inspire and educate scientists who are designing next-generation ADCs with the potential to transform the lives of patients.
Quantitative Phase Imaging Of Cells And Tissues
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Author : Gabriel Popescu
language : en
Publisher: McGraw Hill Professional
Release Date : 2011-03-14
Quantitative Phase Imaging Of Cells And Tissues written by Gabriel Popescu and has been published by McGraw Hill Professional this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-03-14 with Technology & Engineering categories.
Cutting-edge quantitative phase imaging techniques and their applications Filled with unique, full-color images taken by advanced quantitative phase imaging (QPI), Quantitative Phase Imaging of Cells and Tissues thoroughly explores this innovative technology and its biomedical applications. An introductory background on optical imaging and traditional optical microscopy is included to illustrate concept development. The book explains how various visualization modalities can be obtained by numerical calculations. This authoritative resource reveals how to take full advantage of the unprecedented capabilities of QPI, such as rendering scattering properties of minute subcellular structures and nanoscale fluctuations in live cells. Coverage includes: Groundwork Spatiotemporal field correlations Image characteristics Light microscopy Holography Point scanning QPI methods Principles of full-field QPI Off-axis full-field methods Phase-shifting techniques Common-path methods White light techniques Fourier transform light scattering (FTLS) Current trends in QPI