A Comprehensive Analysis Of Human Neural Cell Surface Antigens
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A Comprehensive Analysis Of Human Neural Cell Surface Antigens
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Author : Vishal Menon
language : en
Publisher:
Release Date : 2018
A Comprehensive Analysis Of Human Neural Cell Surface Antigens written by Vishal Menon and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with categories.
Neural Surface Antigens
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Author : Jan Pruszak
language : en
Publisher: Academic Press
Release Date : 2015-03-23
Neural Surface Antigens written by Jan Pruszak and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-03-23 with Science categories.
Neural Surface Antigens: From Basic Biology towards Biomedical Applications focuses on the functionalrole of surface molecules in neural development, stem cell research, and translational biomedical paradigms.With an emphasis on human and rodent model systems, this reference covers fundamentals of neural stemcell biology and flow cytometric methodology. Addressing cell biologists as well as clinicians working in theneurosciences, the book was conceived by an international panel of experts to cover a vast array of particularsurface antigen families and subtypes. It provides insight into the basic biology and functional mechanisms ofneural cell surface signaling molecules influencing mammalian development, regeneration, and treatments. Introduces early phase clinical trials of neural stem cells Outlines characterization of surface molecule expression and methods for isolation which open unprecedented opportunities for functional study, quantitation & diagnostics Highlights the role of stem cells in neural surface antigen and biomarker analysis and applications
Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein 1 Cd71 As A Negative Selection Marker For Human Neuronal Cells
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Author : Vishal Menon
language : en
Publisher:
Release Date : 2019
Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein 1 Cd71 As A Negative Selection Marker For Human Neuronal Cells written by Vishal Menon and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with categories.
Abstract: Cell state-, developmental stage-, and lineage-specific combinatorial expression of cluster of differentiation (CD) molecules enables the identification of cellular subsets via multicolor flow cytometry. We describe an exhaustive characterization of neural cell types by surface antigens, exploiting human pluripotent stem cell-derived neural cell systems. Using multiwell screening approaches followed by detailed validation of expression patterns and dynamics, we exemplify a strategy for resolving cellular heterogeneity in stem cell paradigms. In addition to providing a catalog of surface antigens expressed in the neural lineage, we identified the transferrin receptor-1 (CD71) to be differentially expressed in neural stem cells and differentiated neurons. In this context, we describe a role for N-Myc proto-oncogene (MYCN) in maintaining CD71 expression in proliferating neural cells. We report that in vitro human stem cell-derived neurons lack CD71 surface expression and that the observed differential expression can be used to identify and enrich CD71− neuronal derivatives from heterogeneous cultures
Molecular Biology Of The Cell
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Author : Bruce Alberts
language : en
Publisher:
Release Date : 2002
Molecular Biology Of The Cell written by Bruce Alberts and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Cytology categories.
Human Na Ve Pluripotent Stem Cells
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Author : Peter Rugg-Gunn
language : en
Publisher: Humana
Release Date : 2021-12-07
Human Na Ve Pluripotent Stem Cells written by Peter Rugg-Gunn and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-12-07 with Science categories.
This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Human Naïve Pluripotent Stem Cells: Methods and Protocols is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field.
Stem Cells And The Future Of Regenerative Medicine
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Author : Institute of Medicine
language : en
Publisher: National Academies Press
Release Date : 2002-01-25
Stem Cells And The Future Of Regenerative Medicine written by Institute of Medicine and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-01-25 with Science categories.
Recent scientific breakthroughs, celebrity patient advocates, and conflicting religious beliefs have come together to bring the state of stem cell researchâ€"specifically embryonic stem cell researchâ€"into the political crosshairs. President Bush's watershed policy statement allows federal funding for embryonic stem cell research but only on a limited number of stem cell lines. Millions of Americans could be affected by the continuing political debate among policymakers and the public. Stem Cells and the Future of Regenerative Medicine provides a deeper exploration of the biological, ethical, and funding questions prompted by the therapeutic potential of undifferentiated human cells. In terms accessible to lay readers, the book summarizes what we know about adult and embryonic stem cells and discusses how to go about the transition from mouse studies to research that has therapeutic implications for people. Perhaps most important, Stem Cells and the Future of Regenerative Medicine also provides an overview of the moral and ethical problems that arise from the use of embryonic stem cells. This timely book compares the impact of public and private research funding and discusses approaches to appropriate research oversight. Based on the insights of leading scientists, ethicists, and other authorities, the book offers authoritative recommendations regarding the use of existing stem cell lines versus new lines in research, the important role of the federal government in this field of research, and other fundamental issues.
Human Herpesviruses
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Author : Ann Arvin
language : en
Publisher: Cambridge University Press
Release Date : 2007-08-16
Human Herpesviruses written by Ann Arvin and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-16 with Medical categories.
This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.
The Molecular Biology Of Neurological Disease
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Author : Roger N. Rosenberg
language : en
Publisher: Butterworth-Heinemann
Release Date : 2013-10-22
The Molecular Biology Of Neurological Disease written by Roger N. Rosenberg and has been published by Butterworth-Heinemann this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-10-22 with Medical categories.
The Molecular Biology of Neurological Disease reviews advances that have been made in understanding the molecular mechanisms of neurological disorders as well as immediate and future applications of molecular biological techniques to clinical practice. This book explores the molecular genetics of neurological disease such as muscular dystrophy, Joseph disease, and Huntington's disease, along with the mitochondrial genes implicated in such conditions. This text is comprised of 18 chapters and begins by introducing the reader to the basic principles and methods of molecular genetic techniques used in the diagnosis of neurological disease. Attention then turns to several aspects of genetic expression in the brain, including the extent to which the genome is expressed in the brain. The next chapter focuses on the visualization of polyadenylated messenger RNAs in individual cells in mammalian brain using in situ hybridization techniques, combined with immunohistochemical localization of specific proteins and neuropeptides implicated in diseases such as Alzheimer dementia. This book also discusses the molecular biology of chemical synaptic neurotransmission; proteins involved in the regulation of nervous system development; and gene expression in skeletal muscle. This text then concludes with a summary of the ""neurological gene map"" as it stands in the latter part of 1987. This book is intended for physicians who grapple with the problems of neurological disorders on a daily basis, including neurologists, neurologists in training, and those in related fields such as neurosurgery, internal medicine, psychiatry, and rehabilitation medicine.
Cell Surface Engineering
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Author : Rawil Fakhrullin
language : en
Publisher: Royal Society of Chemistry
Release Date : 2014-07-24
Cell Surface Engineering written by Rawil Fakhrullin and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-07-24 with Medical categories.
A summary of the recent achievements in surface-functionalised cells including fabrication, characterisation, applications and nanotoxicity.
Neuronal Cell Culture
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Author : Shohreh Amini
language : en
Publisher: Humana
Release Date : 2022-06-09
Neuronal Cell Culture written by Shohreh Amini and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-06-09 with Medical categories.
This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.