A Flexible System Of Enzymatic Analysis
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Enzymatic Analysis
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Author : Janet V. Passonneau
language : en
Publisher: Springer Science & Business Media
Release Date : 1993-02-08
Enzymatic Analysis written by Janet V. Passonneau and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-02-08 with Medical categories.
This new edition of a classic laboratory manual covers the general principles, specific methods and procedures, and quantitative histochemistry of enzymatic analysis. It presents a systematic scheme for analyzing biological materials and explains the theory and techniques in terms simple enough for anyone to follow. The protocols are written in a clear, easy to follow style as if the author had just performed the technique himself and knows exactly the problems to be encountered.
A Flexible System Of Enzymatic Analysis
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Author : Oliver Lowry
language : en
Publisher: Elsevier
Release Date : 2012-12-02
A Flexible System Of Enzymatic Analysis written by Oliver Lowry and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
A Flexible System of Enzymatic Analysis is a multipurpose manual of laboratory methods that offers a systematic scheme for the analysis of biological materials from the level of the wholeorgan down to the single cell and beyond. It intends to guide the development of methods, the refinement of old ones, and the adaptation in general of methods to almost any scale of sensitivity. This manual is organized into three parts: a general section, one on quantitative histochemistry, and an appendix containing information that may be useful to have at the bench. The general section is comprised of nine chapters that focus on properties of the pyridine nucleotides, kinetics, and glassware, as well as on improvement, modification, adaptation, trouble shooting, and development of methods. This part also describes the preparation of tissues for analysis, the enzymatic cycling methods, and a compendium of 36 metabolite assays. The quantitative histochemistry section is comprised of four chapters that include information on the preparation of frozen-dried material and dissection of samples for analysis; the fishpole balance for weighing samples; and the generalities of analysis with emphasis on the ""oil well technique."" This book will be useful to novices as well as experts who are familiar with other analytical styles.
Enzymatic Analysis
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Author : Janet V. Passonneau
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-07
Enzymatic Analysis written by Janet V. Passonneau and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-07 with Science categories.
Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.
Methods Of Enzymatic Analysis
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Author : Hans-UIrich Bergmeyer
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Methods Of Enzymatic Analysis written by Hans-UIrich Bergmeyer and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Methods of Enzymatic Analysis focuses on the general progress in enzymology and in the special field of enzymatic analysis. This book explores the commercial production of biochemical reagents for analysis and explains the transition from the possible use of enzymatic analysis to its various applications in pure and applied biochemistry. Organized into four sections, this book starts with an overview of the basis of enzymatic analysis and provides general experimental guidelines for the techniques of measurement and for the disintegration of cells and tissues. This text then provides detailed instructions for the determination of substrates and assay of enzyme activities. Other chapters explore the practical aspects and information necessary for the application of reagents to enzymatic analysis, including sources, stability, and purity required. The final section describes the commercially available enzymes, coenzymes, substrates, and several less common reagents. Biochemists, biophysicists, researchers, and graduate students will find this book extremely useful.
Methods Of Enzymatic Analysis Methods Of Enzymatic Analysis
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Author : Hans-Ulrich Bergmeyer
language : en
Publisher: Wiley-Blackwell
Release Date : 1985-12-27
Methods Of Enzymatic Analysis Methods Of Enzymatic Analysis written by Hans-Ulrich Bergmeyer and has been published by Wiley-Blackwell this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985-12-27 with Science categories.
Methods Of Enzymatic Analysis Fundamentals
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Author : Hans Ulrich Bergmeyer
language : en
Publisher:
Release Date : 1983
Methods Of Enzymatic Analysis Fundamentals written by Hans Ulrich Bergmeyer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983 with Enzymatic analysis categories.
Methods Of Enzymatic Analysis Methods Of Enzymatic Analysis
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Author : Hans-Ulrich Bergmeyer
language : en
Publisher: Wiley-Blackwell
Release Date : 1983-12-23
Methods Of Enzymatic Analysis Methods Of Enzymatic Analysis written by Hans-Ulrich Bergmeyer and has been published by Wiley-Blackwell this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983-12-23 with Science categories.
Methods Of Enzymatic Analysis V2
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Author : Hans-UIrich Bergmeyer
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Methods Of Enzymatic Analysis V2 written by Hans-UIrich Bergmeyer and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Methods of Enzymatic Analysis, Volume 2 reviews developments in the determination of enzyme activity, including advances in assay techniques. It discusses the principles on which measurements of enzymes are based, with each chapter including equations and each method consisting of the pipetting protocol. This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on oxidoreductases, such as sorbitol dehydrogenase, lactate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, 6-phosphogluconate dehydrogenase, xanthine oxidase, and glutamate dehydrogenase. Part II is concerned with transferases ranging from ornithine carbamoyltransferase and transamidinase to transketolase, transaldolase, UDP-glucuronyltransferase, glutamate-pyruvate transaminase, and phosphotransferases. Part III discusses hydrolases including esterases, glycoside hydrolases, peptidases, and proteinases, whereas Part IV looks at lyases, isomerases, and ligases, such as fructose-1, 6-diphosphate aldolase, 1-phosphofructoaldolase, glucosephosphate isomerase, and tetrahydrofolate formylase. This book is a valuable resource for biochemists as well as students and researchers working in the field of analytical biochemistry.
Methods Of Enzymatic Analysis
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Author : Hans Ulrich Bergmeyer
language : en
Publisher:
Release Date : 1983
Methods Of Enzymatic Analysis written by Hans Ulrich Bergmeyer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983 with Enzymatic analysis categories.
Methods Of Enzymatic Analysis Methods Of Enzymatic Analysis
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Author : Hans-Ulrich Bergmeyer
language : en
Publisher: Wiley-Blackwell
Release Date : 1983-11-07
Methods Of Enzymatic Analysis Methods Of Enzymatic Analysis written by Hans-Ulrich Bergmeyer and has been published by Wiley-Blackwell this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983-11-07 with Science categories.