A Low Cost Approach To Pcr
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A Low Cost Approach To Pcr
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Author : Eva Harris
language : en
Publisher: Oxford University Press
Release Date : 1998-12-31
A Low Cost Approach To Pcr written by Eva Harris and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-12-31 with Medical categories.
The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, phylogeny, and forensics. However, despite the large impact of PCR, many of its applications remain within the confines of research and the academic environment. Now, in A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques, Dr. Eva Harris makes this elegantly simple technique more accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad. In addition, the low-cost approach outlined in this book can be useful for high school, undergraduate, or continuing education programs in the United States. While the specific applications of PCR outlined in the book are immediately useful to the study of infectious diseases, the approach presented can be generalized to a number of other technologies and situations. The book will help laboratories in many areas of the world generate information on site for use by physicians, epidemiologists, public health workers, and health policy professionals to develop new strategies for disease control.
A Low Cost Approach To Pcr
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Author : Eva Harris
language : en
Publisher:
Release Date : 1998
A Low Cost Approach To Pcr written by Eva Harris and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Molecular biology categories.
Pcr
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Author : M. J. McPherson
language : en
Publisher: Oxford University Press, USA
Release Date : 1991
Pcr written by M. J. McPherson and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with Language Arts & Disciplines categories.
Relatively simple to perform, yet extremely diverse in tis applications, polymerase chain reaction (PCR) has become an important tool in many areas of biomedical research. It enables scientists to produce large quantities of DNA from minute amounts of starting material. This book provides a general introduction to PCR for researchers new to the area. It covers gene analysis at the level of restriction enzyme polymorphism, point mutation and sequence analysis. Methods for using different source materials - cloned DNA, genomic DNA, RNA, nucleic acid from archive material and PCR products themselves - are given. The emphasis throughout is on the practical applications of the technique and detailed experimental protocols are included at all stages, as well as valuable advice on avoiding pitfalls and optimizing results. Although other manuals exist, this is the most up-to-date work of its kind and has the advantage of being presented in the popular Practical Approach format. It will be of value to researchers and advanced students working in a wide variety of fields, from molecular biology to medicine.
A New Polymer Lab On A Chip For Polymerase Chain Reaction Pcr Using Non Contact Infrared Thermocycles
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Author :
language : en
Publisher:
Release Date : 2005
A New Polymer Lab On A Chip For Polymerase Chain Reaction Pcr Using Non Contact Infrared Thermocycles written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with categories.
In this work, a new polymer Lab-on-a-chip for polymerase chain reaction (PCR) has been developed and characterized using infrared (IR) based thermocycles. These new polymer PCR chips are made by Polycarbonate (PC) and Cyclic Olefin Copolymer (COC). Both of them are designed to have a microchannel for externally inserting a thermocouple to detect temperature during thermocycling. So, these polymer PCR chips can be controlled without using an on-chip integrated temperature sensor and heater, which allows the polymer PCR chips to be a fully disposable platform for low cost PCR chips. The infrared (IR) based heating method also has advantages from not only the non-contact heating but also the fast heating and cooling times. Although PC and COC polymers have a low thermal conductivity compared with Si and glass, the heat energy attained from infrared lamp is enough to successfully perform the thermocycles for PCR. The entire system consists of an IR heating source, an externally insertable thermocouple, a signal conditioning circuit, and Labview control system. The IR heating source is a commercial tungsten halogen lamp which operates at regulated brightness levels for controlling a constant temperature during dwell times and turned off for cooling step. Cooling is accelerated by an air microfan. The overall control of the system including signal conditioning is performed by a Labview program through GPIB cable. In order to obtain the result of PCR amplification from the developed polymer chips, the effective annealing temperature of primer sets and BSA concentration in PCR cocktails on PCR has been explored and optimized. As a result, these polymer PCR chips achieve the successful amplifications of 195 and 544 base pair of e phase DNA. These polymer PCR chips can be immediately used for PCR amplification and also can be applied for constructing a whole system for performing consecutive biochemical processes such as DNA sampling, purification, amplification, and then analysis. With these approaches, a fully disposable polymer PCR chip with the sampling and analysis capabilities of DNA will be feasible.
Intelligent Real Time Polymerase Chain Reaction System With Integrated Nucleic Acid Extraction For Point Of Care Medical Diagnostics
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Author : Tchamie Kadja
language : en
Publisher:
Release Date : 2023
Intelligent Real Time Polymerase Chain Reaction System With Integrated Nucleic Acid Extraction For Point Of Care Medical Diagnostics written by Tchamie Kadja and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023 with categories.
The recent pandemic of Corona-virus Disease 2019 (COVID-19) caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) showed an urgent need to rapidly and accurately identify the genetic material of SARS-CoV-2, an enveloped ribonucleic acid (RNA) virus, in upper respiratory specimens from people. Further, foodborne and waterborne diseases are not only spreading faster, but also appear to be emerging more rapidly than ever before and are able to circumvent conventional control measures. The Polymerase Chain Reaction (PCR) system is a well-known diagnostic tool for many applications in medical diagnostics, environmental monitoring, and food and water quality assessment. Here, we describe the design, development, and testing of a portable, low-cost, and real-time PCR system that can be used in emergency health crises and resource-poor situations. The described PCR system incorporates real-time reaction monitoring using fluorescence as an alternative to gel electrophoresis for reaction analysis, further decreasing the need of multiple reagents, reducing sample testing cost, and reducing sample analysis time. The bill of materials cost of the described system is approximately $340. The described PCR system utilizes a novel progressive selective proportional-integral-derivative controller that helps in reducing sample analysis time. In addition, the system employs a novel primer-based approach to quantify the initial target amplicon concentration, making it well-suited for food and water quality assessment. The developed PCR system performed DNA amplification at a level and speed comparable to larger and more expensive commercial table-top systems. The fluorescence detection sensitivity was also tested to be at the same level as commercially available multi-mode optical readers, thus making the PCR system an attractive solution for medical point-of-care and food and water quality assessment. In general, sensitive testing methods require genetic material extraction from the specimen. Unfortunately, current commercially available extraction kits are expensive and involve time-consuming and laborious extraction procedures. To overcome the difficulties associated with common extraction methods, we propose a simple enzymatic assay for the nucleic acid extraction step using heat mediation to improve the PCR reaction sensitivity. Our protocol was tested on Human Coronavirus 229E (HCoV-229E) as an example, which comes from the large coronaviridae family of viruses that affect birds, amphibians, and mammals, of which SARS-CoV-2 is a member. The proposed assay was performed using a low-cost, custom-made, real-time PCR system that incorporates thermal cycling and fluorescence detection. It had fully customizable reaction settings to allow versatile biological sample testing for various applications, including point-of-care medical diagnosis, food and water quality testing, and emergency health situations. Our results show that heat-mediated RNA extraction is a viable extraction method when compared to commercial extraction kits. Further, our study showed that extraction has a direct impact on purified laboratory samples of HCoV-229E, but no direct impact on infected human cells. This is clinically relevant, as it allows us to circumvent the extraction step on clinical samples when using PCR. Last, traditional testing methods require some external expertise to interpret reaction results. This is a limiting factor to ubiquitous medical testing since low-resource areas may lack such expertise or personnel. The use of Artificial Neural Networks (ANN) for automatic PCR result analysis was demonstrated on low-power microcontroller and incorporated into the proposed system. This is useful for full system autonomy when employing PCR for low-resource point-of-care testing.
Biotechnological Approaches In Food Adulterants
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Author : Madan L Verma
language : en
Publisher: CRC Press
Release Date : 2020-11-18
Biotechnological Approaches In Food Adulterants written by Madan L Verma and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-11-18 with Technology & Engineering categories.
The book highlights the biotechnological advancement in the area of food adulterants and outlines the current state of art technologies in the detection of food adulterants using omics and nanobiotechnology. The book provides insights to the most recent innovations, trends, concerns, and challenges in food adulterants. It identifies key research topics and practical applications of modern cutting-edge technologies employed for detection of food adulterants including: expansion of food adulterants market, potential toxicity of food adulterants and the prevention of food adulteration act, cutting-edge technology for food adulterants detection, and biosensing and nanobiosensing based detection of food adulterants. There is need for new resources in omics technologies for the application of new nanobiotechnology. Biotechnological Approaches in Food Adulterants provides an overview of the contributions of food safety and the most up-to-date advances in omics and nanobiotechnology approaches to a diverse audience from postgraduate students to researchers in biochemical engineering, biotechnology, food technologist, environmental technologists, and pharmaceutical professionals.
Animal Biotechnology
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Author : Ashish S. Verma
language : en
Publisher: Academic Press
Release Date : 2020-06-23
Animal Biotechnology written by Ashish S. Verma and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-06-23 with Medical categories.
Animal Biotechnology: Models in Discovery and Translation, Second Edition, provides a helpful guide to anyone seeking a thorough review of animal biotechnology and its application to human disease and welfare. This updated edition covers vital fundamentals, including animal cell cultures, genome sequencing analysis, epigenetics and animal models, gene expression, and ethics and safety concerns, along with in-depth examples of implications for human health and prospects for the future. New chapters cover animal biotechnology as applied to various disease types and research areas, including in vitro fertilization, human embryonic stem cell research, biosensors, enteric diseases, biopharming, organ transplantation, tuberculosis, neurodegenerative disorders, and more. Highlights the latest biomedical applications of genetically modified and cloned animals, with a focus on cancer and infectious diseases Offers first-hand accounts of the use of biotechnology tools, including molecular markers, stem cells, animal cultures, tissue engineering, ADME and CAM Assay Includes case studies that illustrate safety assessment issues, ethical considerations, and intellectual property rights associated with the translation of animal biotechnology studies
Present Knowledge In Food Safety
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Author : Michael E. Knowles
language : en
Publisher: Academic Press
Release Date : 2022-10-08
Present Knowledge In Food Safety written by Michael E. Knowles and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-10-08 with Medical categories.
Present Knowledge in Food Safety: A Risk-Based Approach Through the Food Chain presents approaches for exposure-led risk assessment and the management of changes in the chemical, pathogenic microbiological and physical (radioactivity) contamination of ’food’ at all key stages of production, from farm to consumption. This single volume resource introduces scientific advances at all stages of the production to improve reliability, predictability and relevance of food safety assessments for the protection of public health. This book is aimed at a diverse audience, including graduate and post-graduate students in food science, toxicology, microbiology, medicine, public health, and related fields. The book's reach also includes government agencies, industrial scientists, and policymakers involved in food risk analysis. Includes new technologies such as nanotechnology, genetic modification, and cloning Provides information on advances in pathogen risk assessment through novel and real-time molecular biological techniques, biomarkers, resistance measurement, and cell-to-cell communication in the gut Covers the role of the microbiome and the use of surrogates (especially for viruses)
Development Of Practical Methods To Assess The Presence Of Bacterial Pathogens In Water
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Author : W. A. Yanko
language : en
Publisher: IWA Publishing
Release Date : 2004-03-01
Development Of Practical Methods To Assess The Presence Of Bacterial Pathogens In Water written by W. A. Yanko and has been published by IWA Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-03-01 with Science categories.
The objective of this project was to develop and evaluate a practical technique to indicate the probability of the presence of bacterial pathogens in receiving waters. A practical method was defined as one that would limit the use of defined culture-based microbiological methods, and would be based on: (1) validating indicator organisms that predicted the presence of pathogens, or (2) detection of pathogens by polymerase chain reaction (PCR)-based assays. The study also assessed the utility of PCR-based technology for bacterial pathogen detection with respect to technology transfer to a wider range of water and wastewater facilities.
Practical Healthcare Epidemiology
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Author : Ebbing Lautenbach
language : en
Publisher: Cambridge University Press
Release Date : 2018-04-19
Practical Healthcare Epidemiology written by Ebbing Lautenbach and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-19 with Medical categories.
A clear, hands-on outline of best practices for infection prevention that directly improve patient outcomes across the healthcare continuum.