Acetogenesis

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Acetogenesis

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Author : Harold L. Drake
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Acetogenesis written by Harold L. Drake and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

This is the first volume to provide comprehensive coverage of acetogenesis. Leading experts present state-of-the-art research, investigating the structure and function relationship of key acetogenic processes, including synthesis of ATP, the conservation of energy, and intersecting metabolic pathways. It explores the interactions between acetogenic bacteria and the flow of matter and energy, giving the reader an integrated perspective on acetogens and the environment. It presents landmark advances in the biochemistry, phylogeny, and molecular biology of acetogens, with lucid explanations of background material, making this volume accessible to newcomers to the subject. It discusses diverse functions of acetogenesis in soils, and gastrointestinal tracts, as well as current and potential commercial and environmental applications. This volume will serve as a broad and in-depth review of basic and emerging issues in acetogenesis for microbiologists, molecular biologists, biochemists, and ecologists, and as a stimulus for future research.

Multienzyme Assemblies And Dynamics In Acetogenesis And Methanogenesis

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Author : Steven E. Cohen (Ph. D.)
language : en
Publisher:
Release Date : 2020

Multienzyme Assemblies And Dynamics In Acetogenesis And Methanogenesis written by Steven E. Cohen (Ph. D.) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with categories.

The Wood-Ljungdahl pathway of acetogenesis allows for growth utilizing carbon dioxide as the sole carbon source. This process uses a series of metalloenzymes to catalyze the reduction of two molecules of carbon dioxide to acetyl-CoA. Key to this pathway are the bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase (CODH/ACS) and corrinoid iron-sulfur protein (CFeSP). CODH/ACS is a bifunctional enzyme, with the CODH subunit catalyzing the reduction of carbon dioxide to carbon monoxide. CFeSP is a cobalt-containing corrinoid-dependent methyltransferase. The active site of ACS, termed the A-cluster, is a nickel, iron, and sulfur-containing metallocluster which catalyzes the synthesis of acetyl-CoA from carbon monoxide provided by CODH, a methyl group provided by CFeSP, and CoA. Despite the unprecedented organometallic chemistry performed at the A-cluster much is unknown about this ACS activity. No substrate-bound structures of ACS have ever been reported and the molecular basis for ACS and CFeSP interactions are largely uncharacterized. Here we present the structure of the carbonylated A-cluster in CODH/ACS, highlighting the role of conformational dynamics in catalysis. We further characterize ACS dynamics using negative-stain electron microscopy, demonstrating a much larger extent of conformational flexibility than has been crystallographically observed. The basis for CFeSP:ACS interactions is further interrogated using a series of CFeSP domain constructs, showing the corrinoidbinding Rossmann fold to be sufficient for ACS interactions. Finally, we report preliminary structural characterization of acetyl-CoA decarbonylase/synthase (ACDS), a 2.2 MDa complex of CODH, ACS, and CFeSP found in archaeal methanogens. Together, this advances our understanding of the roles of structure and dynamics in the Wood-Ljungdahl pathway.

One Carbon Acetogenesis And Autotrophy With Clostridium Thermoautotrophicum

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Author : Maurin Dean Savage
language : en
Publisher:
Release Date : 1986

One Carbon Acetogenesis And Autotrophy With Clostridium Thermoautotrophicum written by Maurin Dean Savage and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986 with Anaerobic bacteria categories.

The Effect Of Different Inhibitors On Dechlorination Methanogenesis And Acetogenesis In An Anaerobic Pce Fed Culture

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Author : Anna P. Carney
language : en
Publisher:
Release Date : 1995

The Effect Of Different Inhibitors On Dechlorination Methanogenesis And Acetogenesis In An Anaerobic Pce Fed Culture written by Anna P. Carney and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.

Effect Of Toxic Organic Chemicals On The Kinetics Of Acetogenesis In Anaerobic Digestion

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Author : In Soo Kim
language : en
Publisher:
Release Date : 1991

Effect Of Toxic Organic Chemicals On The Kinetics Of Acetogenesis In Anaerobic Digestion written by In Soo Kim and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with Anaerobic bacteria categories.

Methanogenesis And Acetogenesis From Methanol

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Author : A. van Langerak
language : en
Publisher:
Release Date : 1991

Methanogenesis And Acetogenesis From Methanol written by A. van Langerak and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with categories.

Autotrophic Acetogenesis In Ruminants

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Author : Joan Torrent i Campmany
language : en
Publisher:
Release Date : 1994

Autotrophic Acetogenesis In Ruminants written by Joan Torrent i Campmany and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Autotrophic bacteria categories.

Acetogenesis From Co2 In An Anoxic Marine Sediment

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Author : T.M. HOEHLER
language : en
Publisher:
Release Date : 1999

Acetogenesis From Co2 In An Anoxic Marine Sediment written by T.M. HOEHLER and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with categories.

Reductive Acetogenesis In The Rumen And Human Gastro Intestinal Microbial Ecosystems

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Author : L. Nollet
language : en
Publisher:
Release Date : 1998

Reductive Acetogenesis In The Rumen And Human Gastro Intestinal Microbial Ecosystems written by L. Nollet and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with categories.

Electron Carriers Involved In Autotrophic And Heterotrophic Acetogenesis In The Thermophilic Bacterium Thermoanaerobacter Kivui

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Author : Alexander Katsyv
language : en
Publisher:
Release Date : 2021

Electron Carriers Involved In Autotrophic And Heterotrophic Acetogenesis In The Thermophilic Bacterium Thermoanaerobacter Kivui written by Alexander Katsyv and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021 with categories.

Thermoanaerobacter kivui is an acetogenic model organism that reduces CO2 with electrons derived from H2 or CO, or from organic substrates in the Wood-Ljugdahl pathway (WLP). For the calculation of ATP yields, it is necessary to know the electron carriers involved in coupling of the oxidative and reductive parts of metabolism. Analyses of key catabolic oxidoreductases in cell-free extract (CFE) or with purified enzymes revealed the physiological electron carriers involved. The glyceraldehyde-3-phosphate dehydrogenase (GA3P-DH) assayed in CFE was NAD+-specific, NADP+ was used with less than 4% and ferredoxin (Fd) was not used. The methylene-THF dehydrogenase was NADP+-specific, NAD+ or Fd were not used. A Nfn-type transhydrogenase that catalyzes reduced Fd-dependent reduction of NADP+ with NADH as electron donor was also identified in CFE. The electron carriers used by the potential electron-bifurcating hydrogenase (HydABC) could not be unambiguously determined in CFE for technical reasons. Therefore, the enzyme was produced homologously in T. kivui and purified by affinity chromatography. HydABC contained 33.9)±)4.5 mol Fe/mol of protein and FMN; it reduced NADP+ but not NAD+. The methylene-THF reductase (MetFV) was also produced homologously in T. kivui and purified by affinity chromatography. MetFV contained 7.2)±)0.4 mol Fe/mol of protein and FMN; the complex did neither use NADPH nor NADH as reductant but only reduced Fd. In sum, these analysis allowed us to propose a scheme for entire electron flow and bioenergetics in T. kivui.