Adrenomedullin As A Modulator Of Human Trophoblast Invasion
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Adrenomedullin As A Modulator Of Human Trophoblast Invasion
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Author : Siu-tak Wong
language : en
Publisher:
Release Date : 2012
Adrenomedullin As A Modulator Of Human Trophoblast Invasion written by Siu-tak Wong and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Adrenomedullin categories.
Adrenomedullin As A Modulator Of Human Trophoblast Invasion
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Author : Siu-Tak Wong
language : en
Publisher: Open Dissertation Press
Release Date : 2017-01-26
Adrenomedullin As A Modulator Of Human Trophoblast Invasion written by Siu-Tak Wong and has been published by Open Dissertation Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-01-26 with categories.
This dissertation, "Adrenomedullin as a Modulator of Human Trophoblast Invasion" by Siu-tak, Wong, 黃兆德, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: During placental development, human trophoblasts differentiate along two pathways leading to the formation of the extravillous cytotrophoblasts (EVCT) and the villous cytotrophoblasts. EVCTis responsible for trophoblast invasion, which is an important process for successfulplacentation in early pregnancy.Dysregulation of the process is associated with a wide range of pregnancy complications, including intrauterine growth restriction, preeclampsia and choriocarcinoma. EVCTsproducematrix metalloproteinase and urokinase plasminogen activator(uPA)that degrade the extracellular matrix of the endometrium for the invasion process. Adrenomedullin is a 52-amino acid polypeptide belonging to the calcitonin/calcitoningene-related peptide/amylin peptide family. The expression of adrenomedullin in trophoblasts is most abundant in the first-trimester human placentas, consistent with the involvement of the molecule in early placentation. Most of the studies on adrenomedullin concentrate on its vasodilatory activity. Studies on the action of adrenomedullin in human trophoblast functions are limited. Therefore the role of adrenomedullin in early placentation is not known. The hypothesis of this project is that adrenomedullin regulates the invasion of human EVCT. Two human EVCT cell lines, JEG-3 and TEV-1 were used as study models in this project. The functions of adrenomedullin aremediated through a receptor system composedof two transmembrane components, the calcitonin receptor-likereceptor and receptor activity-modifying protein(RAMP). Adrenomedullin binds to calcitoninreceptor-likereceptorwhen coupled with RAMP2 or RAMP3. Immunostaining, western blotting and RT-PCR demonstrated the presence of these components on EVCT cell lines. Adrenomedullin treatment significantly enhanced invasiveness, migration, but not proliferation in EVCT as demonstrated by transwell invasion assay, transwell migration assay and cell proliferation assayrespectively. The adrenomedullin antagonist, adrenomedullin22-52, blocked the effects of adrenomedullin on EVCT cell lines, confirming adrenomedullin exerted its biological effects through its classical receptors. RT-PCR results further demonstrated that while adrenomedullin treatment had no effect on matrix metalloproteinase-2 expression, it up-regulated uPA expression and activity. Silencingof uPA by siRNA transfection abolished the stimulatory effect of adrenomedullin, suggesting uPA is the key mediator for adrenomedullin-induced invasion. Adrenomedullin increased nitric oxide synthase expression and nitric oxide (NO) production in JEG-3 cells. The involvement of NO in adrenomedullin-induced EVCT invasion and biosynthesis of uPAwas confirmed bypharmacologicalstudy using nitric oxide synthase inhibitorsor NO donors.Protein kinase G was shown to be one of the downstream regulator of NO that mediated the adrenomedullin-induced EVCT invasion. By using a biotin-switch based method to purify theS-nitrosylated proteins, adrenomedullin was further demonstrate to up-regulate the NO-dependent S-nitrosylation in EVCTs.Interestingly, S-nitrosylation of uPA in vitro induced a higher proteinaseactivitywhich may be responsiblefor the stimulatory action of adrenomedullin. In conclusion, thisstudyhas provided evidence that adrenomedullin modulates EVCT invasion by regulating the uPA expression andactivity through NO signaling pathway. Abnormal trophoblast invasion can le
Adrenomedullin As A Modulator Of Human Trophoblast Invasion
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Author : Siu-tak Wong
language : en
Publisher:
Release Date : 2012
Adrenomedullin As A Modulator Of Human Trophoblast Invasion written by Siu-tak Wong and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Adrenomedullin categories.
Maternal Hemodynamics
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Author : Christoph Lees
language : en
Publisher: Cambridge University Press
Release Date : 2018-05-03
Maternal Hemodynamics written by Christoph Lees and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-03 with Medical categories.
Discover new concepts in cardiovascular and hemodynamic functionality in feto-maternal medicine, from leading experts in the field.
The Human Endometrium
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Author : Carlo Bulletti
language : en
Publisher:
Release Date : 1994
The Human Endometrium written by Carlo Bulletti and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.
The structural, biochemical and clinical events related to menstruation, implantation, parturition, endometriosis, abnormal uterine bleeding and endometrial cancer are discussed in this comprehensive volume on the biological functions of the endometrium. New topics, such as the biochemical and molecular mechanisms regulating maternal embryonic interaction, are explored, and gynecologic endoscopy and therapeutic tools are discussed. The proceedings of the first conference is also available from the Academy, as volume 622 of The Annals of The New York Academy of Science.
Blastocyst Implantation
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Author : Koji Yoshinaga
language : en
Publisher: Adams Publishing Group
Release Date : 1989
Blastocyst Implantation written by Koji Yoshinaga and has been published by Adams Publishing Group this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989 with Medical categories.
Biology And Pathology Of Trophoblast
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Author : Ashley Moffett
language : en
Publisher: Cambridge University Press
Release Date : 2011-08-18
Biology And Pathology Of Trophoblast written by Ashley Moffett and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-18 with Medical categories.
Originally published in 2006, this book provides an in-depth account of trophoblast: the tissue derived from the fertilised egg that nourishes and protects the developing fetus. The cells of the trophoblast have many unique qualities, and exhibit great variability across different species. It has a fascinating role in the development of the placenta and as a regulator during early growth of the embryo. These aspects are all fully covered as well as studies on why it is not rejected by the mother as 'foreign' tissue. Disorders of trophoblast during development also manifest themselves in several clinical conditions during pregnancy, including gestational trophoblastic disease and pre-eclampsia. From stem cells through to epigenetics, implantation and X-chromosome inactivation, there is a lot to be learned about trophoblast, this volume provides a detailed summary of knowledge regarding the subject.
Cumulated Index Medicus
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Author :
language : en
Publisher:
Release Date : 2000
Cumulated Index Medicus written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Medicine categories.
Frontier And Future Development Of Information Technology In Medicine And Education
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Author : Shaozi Li
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-12-05
Frontier And Future Development Of Information Technology In Medicine And Education written by Shaozi Li and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-05 with Technology & Engineering categories.
IT changes everyday’s life, especially in education and medicine. The goal of ITME 2013 is to further explore the theoretical and practical issues of IT in education and medicine. It also aims to foster new ideas and collaboration between researchers and practitioners.
Nitric Oxide Synthase
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Author : Seyed Soheil Saeedi Saravi
language : en
Publisher: BoD – Books on Demand
Release Date : 2017-05-17
Nitric Oxide Synthase written by Seyed Soheil Saeedi Saravi and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-05-17 with Science categories.
Nitric Oxide Synthase - Simple Enzyme-Complex Roles provides information on nitric oxide synthase, a biomolecule of key importance for the different biological systems, including central and peripheral nervous, cardiovascular, and reproductive systems. With recent links to the role of nitric oxide in the reactions that can impact cell signaling, and discoveries surrounding the complex role of nitric oxide synthase that have increased research attention across the fields of cell and molecular biology, physiology, pharmacology, toxicology, neuroscience, cardiology, urology, and endocrinology, this book tries to provide a comprehensive overview of biology/pathobiology of nitric oxide synthases and a perspective from possible therapeutic indication of the enzyme inhibitors.