Advancing The Biosensing Technologies Of Two Optical Label Free Sensing Platforms
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Advancing The Biosensing Technologies Of Two Optical Label Free Sensing Platforms
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Author : Rashmi Sriram
language : en
Publisher:
Release Date : 2015
Advancing The Biosensing Technologies Of Two Optical Label Free Sensing Platforms written by Rashmi Sriram and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
"Optical label-free biosensors promise high-throughput, rapid, and sensitive detection of biomolecules. As such, these biodetection platforms have applications in clinical diagnostics, pharmaceutical industry, homeland security and in environmental monitoring. Motivated by these attributes, my thesis has focused on advancing the biosensing technology of two novel silicon-based optical label-free sensors: arrayed imaging reflectometry (AIR) and two-dimensional photonic crystal structures (2D PhC). The AIR biosensor is an interferometry-based technique that relies on the creation and destruction of a near-zero reflectivity condition in a carefully chosen thin film. To thoroughly quantify the biosensor detection signal, a correlation between the sensor response and the solution analyte concentration needs to be determined. In the first half of this thesis, a theoretical framework was developed to predict the AIR sensor response for a given solution analyte concentration. The developed model was subsequently validated using spectroscopic ellipsometry and surface plasmon resonance techniques in addition to AIR by utilizing two model probe-analyte pairs. It was established that the probe-analyte interaction on AIR substrate across two formats: macrospots and microarrays closely represented one-site and two-site Langmuir models, respectively. Two-dimensional photonic crystals are periodic dielectric structures with spatial periodicity in two dimensions. In the presence of a "defect" microcavity that disrupts the spatial periodicity, light of specific wavelength can be captured within the defect cavity. This manifests as a "dip" in the transmission spectrum of the crystal and is very sensitive to the local refractive index changes in the immediate vicinity of the defect cavity. The second half of this thesis investigates the utility of two designs of the 2D PhC: "small" and "large" point-defect microcavities coupled to a transmission waveguide for sensing latex particles in microfluidic environment. With the "small" defect design, detection of ~100 nm size latex particles in microfluidic environment was confirmed. This is the first study that demonstrates particle detection in the presence of a fluid cover using a 2D PhC sensor, a significant technological advancement for the incorporation of this sensor into lab-on-a-chip device. Using a "large" point-defect cavity design, real-time detection of latex particles of size ~280 nm was demonstrated. This to our knowledge is the first demonstration of detection of latex particles in real-time using 2D PhCs without the aid of any optical forces. This proof-of-principle demonstration of particle detection lays the foundation for the further development of these devices for the detection of a single biological particle, which when accomplished will have a huge impact in the field of clinical diagnostics"--Pages ix-x.
On Chip Biosensing Platforms Based On Gold And Silicon Optical Nano Resonators
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Author : Ozlem Yavas
language : en
Publisher:
Release Date : 2019
On Chip Biosensing Platforms Based On Gold And Silicon Optical Nano Resonators written by Ozlem Yavas and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with categories.
Point-of-care (POC) devices are compact, mobile and fast detection platforms expected to advance early diagnosis, treatment monitoring and personalized healthcare, and revolutionize today's healthcare system, especially in remote areas. The need for POC devices strongly drives the development of novel biosensor technology. Building a small, fast, simple, and sensitive platform for biomolecule detection is a challenge that relies on the integration of multiple fields of expertise and engineering. Optical nanoresonators have shown great promise as label-free biosensors because of direct light coupling and sub-wavelength sensing modes. Metallic nanoresonators with localized surface plasmon resonances (LSPR) are already well studied and were proven a solid alternative to the commercialized surface plasmon resonance (SPR) sensors. More recently, dielectric nanoresonators have also gained traction due to the reduced losses and the ability to manipulate both the electric and magnetic components of the incident light. In this thesis, we advance the field of biosensing and use optical nanoresonators as operative platforms relevant for disease diagnosis and treatment monitoring. By combining different optimized optical nanoresonators, both metallic and dielectric, with state-of-the-art microfluidics and surface chemistry, we have developed and tested several detection platforms. We first focused on developing a microfluidic lab-on-chip device for multiplexed biosensing utilizing the LSPR of gold nanoresonator arrays. By simultaneously tracking the extinction of 32 sensor arrays, we demonstrated multiplexed quantitative detection of four breast cancer markers in human serum. We showed that with well-optimized immunoassays, a low limit of detection (LOD) can be reached, paving the way towards clinically-relevant POC devices. Additionally, we implemented silicon nanoresonators supporting Mie resonances into functional and clinically-relevant applications. By integrating several arrays of Si nanoresonators with state-of-the-art microfluidics, we demonstrated their ability to detect cancer markers in human serum with high sensitivity and high specificity. Furthermore, we showed that the fabrication of Si nanoresonator array using low cost and scalable projection lithography leads to sufficiently low limits of detection, while enabling cheaper and faster sensor production for future POC applications. We also investigated the respective role of electric and magnetic dipole resonances and showed that they are associated with two different transduction mechanisms: resonance redshift and extinction decrease. Our work advances the development of future point-of-care sensing platforms for fast and low cost health monitoring at the molecular scale.
Optical Fiber Biosensors
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Author : Daniele Tosi
language : en
Publisher: Academic Press
Release Date : 2021-11-17
Optical Fiber Biosensors written by Daniele Tosi and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-11-17 with Technology & Engineering categories.
Optical Fiber Biosensors: Device Platforms, Biorecognition, Applications provides a comprehensive overview of the field of fiber optic sensors using an interdisciplinary approach that covers the fabrication of sensing devices and optical hardware, the functionalization to perform selective biorecognition, and the main applications of biosensors, with a present and a future outlook. Chapters discuss the principles of light propagation and the sensing devices suitable to perform biosensing with optical fibers, the process to functionalize the previous devices to selective biosensing, and applications in cells, small molecules, biomarkers and protein sensing, with a birds eye view on the most important results. This book provides a coherent picture of fiber optic biosensors, from the start (the device) to the end (the application), explaining in simple terms what is the whole process for development of a biosensor. The book also contains practical material (e.g. commercial instruments, fabrication instructions, medical standards for biocompatibility) that cannot be easily found elsewhere, and this is very useful for researchers to plan their development and build their labs. Covers the technologies and operating principles of optical fiber devices used in biosensing Contains chapters on the chemistry and operational strategy to functionalize a fiber device to become an effective biosensor Addresses the main applications of fiber optic biosensors and their specialization
Study And Development Of Label Free Optical Biosensors For Biomedical Applications
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Author : Charles J. Choi
language : en
Publisher:
Release Date : 2011
Study And Development Of Label Free Optical Biosensors For Biomedical Applications written by Charles J. Choi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
For the majority of assays currently performed, fluorescent or colorimetric chemical labels are commonly attached to the molecules under study so that they may be readily visualized. The methods of using labels to track biomolecular binding events are very sensitive and effective, and are employed as standardized assay protocol across research labs worldwide. However, using labels induces experimental uncertainties due to the effect of the label on molecular conformation, active binding sites, or inability to find an appropriate label that functions equivalently for all molecules in an experiment. Therefore, the ability to perform highly sensitive biochemical detection without the use of fluorescent labels would further simplify assay protocols and would provide quantitative kinetic data, while removing experimental artifacts from fluorescent quenching, shelf-life, and background fluorescence phenomena. In view of the advantages mentioned above, the study and development of optical label-free sensor technologies have been undertaken here. In general, label-free photonic crystal (PC) biosensors and metal nanodome array surface-enhanced Raman scattering (SERS) substrates, both of which are fabricated by nanoreplica molding process, have been used as the method to attack the problem. Chapter 1 shows the work on PC label-free biosensor incorporated microfluidic network for bioassay performance enhancement and kinetic reaction rate constant determination. Chapter 2 describes the work on theoretical and experimental comparison of label-free biosensing in microplate, microfluidic, and spot-based affinity capture assays. Chapter 3 shows the work on integration of PC biosensor with actuate-to-open valve microfluidic chip for pL-volume combinatorial mixing and screening application. In Chapter 4, the development and characterization of SERS nanodome array is shown. Lastly, Chapter 5 describes SERS nanodome sensor incorporated tubing for point-of-care monitoring of intravenous drugs and metabolites.
Label Free Biosensing
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Author : Michael J. Schöning
language : en
Publisher: Springer
Release Date : 2018-07-20
Label Free Biosensing written by Michael J. Schöning and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-07-20 with Science categories.
This volume summarizes the state-of-the-art technologies, key advances and future trends in the field of label-free biosensing. It provides detailed insights into the different types of solid-state, label-free biosensors, their underlying transducer principles, advanced materials utilized, device-fabrication techniques and various applications. The book offers graduate students, academic researchers, and industry professionals a comprehensive source of information on all facets of label-free biosensing and the future trends in this flourishing field. Highlights of the subjects covered include label-free biosensing with: · semiconductor field-effect devices such as nanomaterial-modified capacitive electrolyte-insulator-semiconductor structures, silicon nanowire transistors, III-nitride semiconductor devices and light-addressable potentiometric sensors · impedimetric biosensors using planar and 3D electrodes · nanocavity and solid-state nanopore devices · carbon nanotube and graphene/graphene oxide biosensors · electrochemical biosensors using molecularly imprinted polymers · biomimetic sensors based on acoustic signal transduction · enzyme logic systems and digital biosensors based on the biocomputing concept · heat-transfer as a novel transducer principle · ultrasensitive surface plasmon resonance biosensors · magnetic biosensors and magnetic imaging devices
Next Generation Smart Biosensing
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Author : Kamil Reza Khondakar
language : en
Publisher: Elsevier
Release Date : 2024-01-14
Next Generation Smart Biosensing written by Kamil Reza Khondakar and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-01-14 with Science categories.
Quantum Sensing at the Interface of Nanotechnology Integrated Microfluidics provides broad multidisciplinary coverage of innovative quantum sensing technologies suitable to industries in the engineering, biomedical, healthcare and environmental sectors. Sections discuss emerging quantum sensing and with an introduction to microfluidic devices, smart sensors, the role of nanotechnology, smart sensing, and the role of quantum technology and artificial intelligence for nano-enabled microfluidics. Sensing technologies and nano-enabled microfluidics and their biomedical and industrial applications are explored. This will be a useful resource for those in research and industry interested in biotechnology, nanotechnology, sensing technology and their applications in multidisciplinary fields. Provides an introduction to the types of microfluidic devices, smart sensors, and the role of nanotechnology Covers smart sensing for multidisciplinary sectors Explores the challenges and prospects of nano-microfluidics systems
Label Free Biosensors
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Author : Matthew A. Cooper
language : en
Publisher: Cambridge University Press
Release Date : 2009-02-02
Label Free Biosensors written by Matthew A. Cooper and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-02-02 with Science categories.
Label-free biosensors are devices that use biological or chemical receptors to detect analytes (molecules) in a sample. They give detailed information on the selectivity, affinity, and, in many cases, also the binding kinetics and thermodynamics of an interaction. Although they can be powerful tools in the hands of a skilled user, there is often a lack of knowledge of the best methods for using label-free assays to screen for biologically active molecules and accurately and precisely characterize molecular recognition events. This book reviews both established and newer label-free techniques giving both the expert user and the general reader interested in the technologies and applications behind label-free an insight into the field from expert opinion leaders and practitioners of the technologies. Most importantly, chapters contain worked examples from leaders in the field that take the reader through the basics of experimental design, setup, assay development, and data analysis.
Optical Whispering Gallery Modes For Biosensing
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Author : Frank Vollmer
language : en
Publisher: Springer Nature
Release Date : 2020-10-16
Optical Whispering Gallery Modes For Biosensing written by Frank Vollmer and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-16 with Science categories.
This interdisciplinary book covers the fundamentals of optical whispering gallery mode (WGM) microcavities, light–matter interaction, and biomolecular structure with a focus on applications in biosensing. Novel biosensors based on the hybridization of WGM microcavities and localized surface plasmon resonances (LSPRs) in metal nanoparticles have emerged as the most sensitive microsystem biodetection technology that boasts single molecule detection capability without the need for amplification and labeling of the analyte. The book provides an ample survey of the physical mechanisms of WGMs and LSPRs for detecting affinity, concentration, size, shape and orientation of biomarkers, while informing the reader about different classes of biomolecules, their optical properties and their importance in label-free clinical diagnostics. For the more advanced reader, advanced applications of WGMs and LSPRs in exploring the fundamental nature of quantum physics are discussed.
Modern Techniques In Biosensors
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Author : Gorachand Dutta
language : en
Publisher: Springer Nature
Release Date : 2021-01-04
Modern Techniques In Biosensors written by Gorachand Dutta and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-01-04 with Technology & Engineering categories.
This book focusses on recent advances and different research issues in the biosensor technology and also presents theoretical, methodological, well-established and validated empirical work dealing with the technology. The book addresses challenges for the development of a point-of-care test platform. The book also describes printed chip-based assay (Lab-on-a-Chip, Lab-on-a-PCB) for rapid, inexpensive, multiplex detection of disease biomarkers in real samples. It aims to overcome existing barriers for Lab-on-a-Chip commercialization (lack of cost effective mass manufacturing methods, self-contained, fully autonomous operation and user-friendliness). Different advanced techniques including electrochemical, optical, mass, colorimetric and signal amplification strategies describe early stage disease diagnosis. The book gathers scientific and technological novelties and advancements already developed or under development in the academic and research communities. It covers a vast audience from basic science to engineering and technology experts and learners.
Nanoplasmonic Sensors
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Author : Alexandre Dmitriev
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-07-31
Nanoplasmonic Sensors written by Alexandre Dmitriev and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-07-31 with Science categories.
This book is a compendium of the finest research in nanoplasmonic sensing done around the world in the last decade. It describes basic theoretical considerations of nanoplasmons in the dielectric environment, gives examples of the multitude of applications of nanoplasmonics in biomedical and chemical sensing, and provides an overview of future trends in optical and non-optical nanoplasmonic sensing. Specifically, readers are guided through both the fundamentals and the latest research in the two major fields nanoplasmonic sensing is applied to – bio- and chemo-sensing – then given the state-of-the-art recipes used in nanoplasmonic sensing research.