Affinity Chromatography

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Affinity Chromatography

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Author : Pascal Bailon
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

Affinity Chromatography written by Pascal Bailon and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.

Affinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.

Affinity Chromatography

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Author : Peter D. G. Dean
language : en
Publisher: Oxford University Press, USA
Release Date : 1985

Affinity Chromatography written by Peter D. G. Dean and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985 with Science categories.

Matrix preparation and applications; Activation procedures; Cross-linking agents for coupling matrices to spacers; Operational methodologies; Ligands for immobilisation; Quantitative characterisation of interactions by affinity chromatography; Zonal elution quantitative affinity chromatography and analysis of molecular interactions; Cell separation by affinity chromatography ligand immobilisation to solid supports through cleavable mercury-sulphur bonds.

Handbook Of Affinity Chromatography

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Author : David S. Hage
language : en
Publisher: CRC Press
Release Date : 2005-07-19

Handbook Of Affinity Chromatography written by David S. Hage and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-07-19 with Science categories.

This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry. The Handbook of Affinity Chromatograph

Bioaffinity Chromatography

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Author : J. Turková
language : en
Publisher: Elsevier
Release Date : 1993-09-14

Bioaffinity Chromatography written by J. Turková and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-09-14 with Science categories.

Bioaffinity chromatography is now the preferred choice for the purification, determination or removal of many biologically active substances. The book includes information on biologically active substances with their affinants, solid supports and methods of coupling, summarized in tables covering classical, high-performance liquid and large-scale bioaffinity chromatography. Optimization of the preparation and the use of highly active and stable biospecific adsorbents is discussed in several chapters. Following a chapter dealing with the choice of affinity ligands, affinity-sorbent bonding is described in detail. Other chapters give information on solid supports, the most common coupling procedures and a general discussion of sorption and elution. Several applications of bioaffinity chromatography are described, e.g. quantitative evaluation of biospecific complexes and many applications in medicine and in the biotechnology industry.

Affinity Chromatography

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Author : O. Hoffmann-Ostenhof
language : en
Publisher: Elsevier
Release Date : 2016-01-22

Affinity Chromatography written by O. Hoffmann-Ostenhof and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-01-22 with Science categories.

Affinity Chromatography is a collection of papers that presents the advancement in various areas of affinity chromatography. The title particularly covers the development in affinity chromatography in the context of biochemistry and immunochemistry. The text first details gel matrices, coupling methods, and charge transfer chromatography. Next, the selection tackles the affinity chromatography of enzymes and hydrophobic interaction chromatography. The text also discusses matrix-bound antigens and antibodies, along with matrix-bound lectins and affinity chromatography of cells. Part VI deals with affinity chromatography of hormone receptors, while part vii talks about affinity techniques in the isolation Of DNA, RNA, and polysomes. The book will be of great use to students, researchers, and practitioners of biochemistry.

Affinity Chromatography

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Author : Sameh Magdeldin
language : en
Publisher: BoD – Books on Demand
Release Date : 2012-03-21

Affinity Chromatography written by Sameh Magdeldin and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-03-21 with Science categories.

Most will agree that one major achievement in the bio-separation techniques is affinity chromatography. This coined terminology covers a myriad of separation approaches that relies mainly on reversible adsorption of biomolecules through biospecific interactions on the ligand. Within this book, the authors tried to deliver for you simplified fundamentals of affinity chromatography together with exemplarily applications of this versatile technique. We have always been endeavor to keep the contents of the book crisp and easily comprehensive, hoping that this book will receive an overwhelming interest, deliver benefits and valuable information to the readers.

Affinity Chromatography

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Author : P. Mohr
language : en
Publisher: CRC Press
Release Date : 1985-11-22

Affinity Chromatography written by P. Mohr and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985-11-22 with Science categories.

Affinity Chromatography combines theoretical aspects and practical applications-providing a solid understanding of affinity principles on a molecular level. Beginning with the historical background of affinity chromatography, this single-source volume discusses matrix supports and the insertion of spacers ... the chemical and physicochemical features of the adsorption and elution step ... immunoassay technology and separation of viruses and cells ... variants of affinity chromatography other than biospecific ... related techniques including affinity electrophoresis ... and much more. Written by leading experts in the field, Affinity Chromatography contains convenient features such as a concise outline format-summarizing useful information; numerous illustrations-clarifying fundamental and methodical factors; easy-to-read tables-for a quick understanding of vital material; current references-facilitating continuing study. Providing stimulation for the development of new affinity methods, this important volume is mandatory for analytical chemists, chromatographers, biochemists, biologists, microbiologists, pharmacists, and students in advanced undergraduate- and graduate-level analytical and preparative chemistry courses. Book jacket.

Analytical Affinity Chromatography

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Author : Irwin M. Chaiken
language : en
Publisher: CRC Press
Release Date : 2018-01-10

Analytical Affinity Chromatography written by Irwin M. Chaiken and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-10 with Science categories.

This volume presents discussions of theoretical and experimental considerations that have led to the analytical affinity chromatography field, as well as current efforts to use this methodology to characterize the interaction mechanisms of biological macromolecules and to establish conditions for employing bioaffinity chromatographic systems as preparative tools. The chapters include a comprehensive discussion of interactive chromatography theory (DeLisi and Hethcote), a review of experimental data obtained for biological macromolecules and the relevant theoretical considerations of affinity chromatography which led to them (Swaisgood and Chaiken), an evaluation of rate processes in affinity chromatography and the potential to determine biologically meaningful chemical rate constants (Walters), and the use of quantitative and molecular considerations to design affinity chromatographic systems (Stellwagen and Liu).

Affinity Chromatography And Biological Recognition

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Author : Irwin Chaiken
language : en
Publisher: Elsevier
Release Date : 2012-12-02

Affinity Chromatography And Biological Recognition written by Irwin Chaiken and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.

Affinity Chromatography and Biological Recognition contains manuscripts presented at the Fifth International Symposium on Affinity Chromatography and Biological Recognition convened in June 12-17, 1983, at St. John's College in Annapolis, Maryland. Organized into six parts encompassing 82 chapters, the book begins by examining the growing synergism between affinity methods and the understanding and study of basic principles of biological recognition. The book then focuses on the trends and progress in the design and application of affinity methods for isolation, therapeutics, diagnostics, and biotechnology. Significant chapters are devoted to the contributions of affinity methodology in such areas as cell membrane receptors, quantitative properties of macromolecular interactions, microscale analytical and preparative applications of high performance affinity chromatography, antibodies as in vivo and in vitro diagnostic and therapeutic agents, and drug targeting. This volume will be a stimulus for broad and creative application of affinity concepts and methods in many fields of biomedical research and biotechnology.

Affinity Chromatography

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Author : Herbert Schott
language : en
Publisher: CRC Press
Release Date : 2020-11-25

Affinity Chromatography written by Herbert Schott and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-11-25 with Science categories.

This book informs the reader about the practical methods, possibilities, and limits of template chromatography. It shows the various techniques for immobilization of nucleic acids fragments, polynucleotides, and nucleic acids by which the desired separation of materials can be achieved.