Application Of Stable Isotope Probing To Identify Rdx Degrading Bacteria In Groundwater
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Application Of Stable Isotope Probing To Identify Rdx Degrading Bacteria In Groundwater
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Author : Kun-Ching Cho
language : en
Publisher:
Release Date : 2014
Application Of Stable Isotope Probing To Identify Rdx Degrading Bacteria In Groundwater written by Kun-Ching Cho and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.
Microbial Degradation Of Xenobiotics
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Author : Shree Nath Singh
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-10-07
Microbial Degradation Of Xenobiotics written by Shree Nath Singh and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-07 with Science categories.
Our interest in the microbial biodegradation of xenobiotics has increased many folds in recent years to find out sustainable ways for environmental cleanup. Bioremediation and biotransformation processes harness the naturally occurring ability of microbes to degrade, transform or accumulate a wide range of organic pollutants. Major methodological breakthroughs in recent years through detailed genomic, metagenomic, proteomic, bioinformatic and other high-throughput analyses of environmentally relevant microorganisms have provided us unprecedented insights into key biodegradative pathways and the ability of organisms to adapt to changing environmental conditions. The degradation of a wide spectrum of organic pollutants and wastes discharged into the environment by anthropogenic activities is an emerging need today to promote sustainable development of our society with low environmental impact. Microbial processes play a major role in the removal of recalcitrant compounds taking advantage of the astonishing catabolic versatility of microorganisms to degrade or transform such compounds. New breakthroughs in sequencing, genomics, proteomics, bioinformatics and imaging are generating vital information which opens a new era providing new insights of metabolic and regulatory networks, as well as clues to the evolution of degradation pathways and to the molecular adaptation strategies to changing environmental conditions. Functional genomic and metagenomic approaches are increasing our understanding of the relative importance of different pathways and regulatory networks to carbon flux in particular environments and for particular compounds. New approaches will certainly accelerate the development of bioremediation technologies and biotransformation processes in coming years for natural attenuation of contaminated environments
Fluorescent Probes
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Author :
language : en
Publisher: Academic Press
Release Date : 2021-05-13
Fluorescent Probes written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-05-13 with Science categories.
Fluorescent Probes, Volume 48 in the Methods in Microbiology series, highlights new advances in the field, with this new volume presenting interesting chapters on important topics, including Hydrogel microarray technology as a tool for clinical diagnostics, The use of probes and bacteriophages for bacteria detection, Probes used with point-of-care microfluidic devices for pathogen detection, Methods for combining FIB/SEM with three-dimensional fluorescence microscopy using CLEM approaches, Probes and Microbes, Microbial signatures associated with cancers, Fluorescent Aptamers for Detection and Treatment of Pathogenic Bacteria and Cancer, Labelled and Unlabeled Probes for Pathogen Detection with Molecular Biology Methods and Biosensors, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Microbiology series
Stable Isotope Probing And Related Technologies
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Author : J. Colin Murrell
language : en
Publisher: American Society for Microbiology Press
Release Date : 2010-12-10
Stable Isotope Probing And Related Technologies written by J. Colin Murrell and has been published by American Society for Microbiology Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-10 with Science categories.
The ideal starting point for investigating, developing, and implementing stable isotope technologies. • Guides researchers through basic, tested, and proven protocols including DNA, RNA, protein, and phospholipid fatty acid (PLFA) SIP, from concept and history through detailed methodology, troubleshooting, and interpretation to optimal and future uses. • Explores important and emerging applications of SIP in environmental microbiology, ranging from bioremediation and gene mining to carbon tracking and gut microflora function. • Examines explorations of further elegant isotope labeling technologies such as Raman-FISH, NanoSIMS, and isotope arrays. • Serves as a valuable resource for environmental microbiology students and researchers and genomics, biotechnology, and medical microbiology professionals.
Biostimulation And Microbial Community Profiling Reveal Insights On Rdx Transformation In Groundwater
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Author :
language : en
Publisher:
Release Date : 2016
Biostimulation And Microbial Community Profiling Reveal Insights On Rdx Transformation In Groundwater written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with categories.
Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a high explosive released to the environment as a result of weapons manufacturing and testing worldwide. At Los Alamos National Laboratory, the Technical Area (TA) 16 260 Outfall discharged high-explosives-bearing water from a high-explosives-machining facility to Cañon de Valle during 1951 through 1996. These discharges served as a primary source of high-explosives and inorganic-element contamination in the area. Data indicate that springs, surface water, alluvial groundwater, and perched-intermediate groundwater contain explosive compounds, including RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine); HMX (octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine); and TNT (2,4,6-trinitrotoluene). RDX has been detected in the regional aquifer in several wells, and a corrective measures evaluation is planned to identify remedial alternatives to protect the regional aquifer. Perched-intermediate groundwater at Technical Area 16 is present at depths from 650 ft to 1200 ft bgs. In this study, we examined the microbial diversity in a monitoring well completed in perched-intermediate groundwater contaminated by RDX, and examined the response of the microbial population to biostimulation under varying geochemical conditions. Results show that the groundwater microbiome was dominated by Actinobacteria and Proteobacteria. A total of 1,605 operational taxonomic units (OTUs) in 96 bacterial genera were identified. Rhodococcus was the most abundant genus (30.6%) and a total of 46 OTUs were annotated as Rhodococcus. One OTU comprising 25.2% of total sequences was closely related to a RDX -degrading strain R. erythropolis HS4. A less abundant OTU from the Pseudomonas family closely related to RDX-degrading strain P. putida II-B was also present. Biostimulation significantly enriched Proteobacteria but decreased/eliminated the population of Actinobacteria. Consistent with RDX degradation, the OTU closely related to the RDX-degrading P. putida strain II-B was specifically enriched in the RDX-degrading samples. Analysis of the accumulation of RDX-degradation products reveals that during active RDX degradation, there is a transient increase in the concentration of the degradation products MNX, DNX, TNX, and NDAB. The accumulation of these degradation products suggests that RDX is degraded via sequential reduction of the nitro functional groups followed by abiotic ring-cleavage. Here, the results suggest that strict anaerobic conditions are needed to stimulate RDX degradation under the TA-16 site-specific conditions.
Stable Isotope Probing
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Author : Marc G. Dumont
language : en
Publisher:
Release Date : 2019
Stable Isotope Probing written by Marc G. Dumont and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with Microbial genetics categories.
This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.
Using Stable Isotope Probing To Identify Soil Microbial Populations Involved In Phenol Metabolism At An Agricultural Field Site
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Author : Christopher M. DeRito
language : en
Publisher:
Release Date : 2007
Using Stable Isotope Probing To Identify Soil Microbial Populations Involved In Phenol Metabolism At An Agricultural Field Site written by Christopher M. DeRito and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with categories.
Identification Of Active Soil Rdx Biodegrading Microorganisms Using 15nitrogen Stable Isotope Probing
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Author : Indumathy Jayamani
language : en
Publisher:
Release Date : 2009
Identification Of Active Soil Rdx Biodegrading Microorganisms Using 15nitrogen Stable Isotope Probing written by Indumathy Jayamani and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with Explosives categories.
Development Of Stable Isotope Probing For The Isolation Of Biocatalytic Genes From Aromatic Compound Degrading Bacteria
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Author : Andrew Richard Frazer
language : en
Publisher:
Release Date : 2012
Development Of Stable Isotope Probing For The Isolation Of Biocatalytic Genes From Aromatic Compound Degrading Bacteria written by Andrew Richard Frazer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.
Toxicological Profile For Rdx
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Author :
language : en
Publisher:
Release Date : 1995
Toxicological Profile For Rdx written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with Explosives categories.