Automated Data Extraction From In Situ Protein Stable Isotope Probing Studies
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Automated Data Extraction From In Situ Protein Stable Isotope Probing Studies
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Author : Gordon W. Slysz
language : en
Publisher:
Release Date : 2014
Automated Data Extraction From In Situ Protein Stable Isotope Probing Studies written by Gordon W. Slysz and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with Bioinformatics categories.
Protein-stable isotope probing (protein-SIP) has strong potential for revealing key metabolizing taxa in complex microbial communities. While most protein-SIP work to date has been performed under controlled laboratory conditions to allow extensive isotope labeling of the target organism(s), a key application will be in situ studies of microbial communities for short periods of time under natural conditions that result in small degrees of partial labeling. One hurdle restricting large-scale in situ protein-SIP studies is the lack of algorithms and software for automated data processing of the massive data sets resulting from such studies. In response, we developed Stable Isotope Probing Protein Extraction Resources software (SIPPER) and applied it for large-scale extraction and visualization of data from short-term (3 h) protein-SIP experiments performed in situ on phototrophic bacterial mats isolated from Yellowstone National Park. Several metrics incorporated into the software allow it to support exhaustive analysis of the complex composite isotopic envelope observed as a result of low amounts of partial label incorporation. SIPPER also enables the detection of labeled molecular species without the need for any prior identification.
Modern Soil Microbiology Third Edition
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Author : Jan Dirk van Elsas
language : en
Publisher: CRC Press
Release Date : 2019-04-05
Modern Soil Microbiology Third Edition written by Jan Dirk van Elsas and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-05 with Technology & Engineering categories.
The living soil is crucial to photosynthesis, biogeochemical cycles, global food production, climate change, biodiversity, and plant and animal health. In the past decade, scientists have made significant advances in soil microbiology research. While the basic principles are now better understood, knowledge has been forthcoming on the best available technologies and methods applied to researching soil microorganisms, their diversity, interactions, biochemistry, survival, gene expression, and their roles in global climate change, plant disease suppression and growth stimulation, and biogeochemical cycles. This knowledge can be applied to better predict the transformation of pollutants in soil and the activities of microbes in the rhizosphere. It will also assist us in fostering crop production in an era with an increasing human population and intensification of agriculture. Following the tradition of its predecessors, Modern Soil Microbiology, Third Edition, is an indispensable source that supports graduate/undergraduate teaching for soil and environmental microbiologists in academia, as well as in government and industrial laboratories. It is a comprehensive collection of chapters on various aspects of soil microbiology, useful for all professionals working with soils. Compiled by internationally renowned educators and research scholars, this textbook contains key tables, figures, and photographs, supported by thousands of references to illustrate the depth of knowledge in soil microbiology. FEATURES Fully updated and expanded to include new key chapters on historical developments, future applications, and soil viruses and proteins Discusses molecular methods applied to soil microbiology, diverse soil microorganisms, and global climate change Emphasizes the role of terrestrial microorganisms and cycles involved in climate change Details the latest molecular methods applied to soil microbiology research User-friendly for students, and containing numerous tables, figures, and illustrations to better understand the current knowledge in soil microbiology
Metagenomics
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Author : Wolfgang R. Streit
language : en
Publisher: Springer Nature
Release Date : 2022-10-28
Metagenomics written by Wolfgang R. Streit and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-10-28 with Science categories.
This volume provides up-to-date and novel techniques for various screening technologies currently used in metagenomics and related areas. Starting with DNA/RNA isolation from environmental samples, the book continues by delving into areas such as current methods used to isolate DNA and construct metagenomic libraries, establishment of metagenome libraries in non-E. coli hosts, and topics like function-driven mining of metagenomic DNA, screening and analyzing protocols for a wide array of different genes encoding enzymes, bacterial viruses and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metagenomics: Methods and Protocols, Third Edition provides a comprehensive collection of up-to-date metagenome protocols and tools for the recovery of many major types of biocatalysts and allows for the easy setup of these screens in microbiology laboratories.
Ovarian Cycle
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Author : Gerald Litwack
language : en
Publisher: Academic Press
Release Date : 2018-03-13
Ovarian Cycle written by Gerald Litwack and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-03-13 with Science categories.
Ovarian Cycle, Volume 107, the latest in the Vitamins and Hormones series first published in 1943, and the longest-running serial published by Academic Press, covers the latest updates on hormone action, vitamin action, X-ray crystal structure, physiology and enzyme mechanisms. This latest release includes an overview of the ovarian cycle, a section on ovarian hyperstimulation syndrome, information on androgens and ovarian follicular maturation, information on peptide inhibitors of human thymidylate synthase to inhibit ovarian cancer cell growth, sections on nodal and luteolysis, neurokinins, dynorphin and pulsatile Lh secretion, Lh receptor expression by Mir12, and gonadotrophin-surge attenuating factor, melatonin and Bmp-6 regulation, amongst other topics. Focuses on the newest aspects of hormone action in connection with diseases Lays the groundwork for the focus of new chemotherapeutic targets Reviews emerging areas in hormone action, cellular regulators and signaling pathways
Anaerobic Utilization Of Hydrocarbons Oils And Lipids
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Author : Matthias Boll
language : en
Publisher: Springer
Release Date : 2020-03-12
Anaerobic Utilization Of Hydrocarbons Oils And Lipids written by Matthias Boll and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-03-12 with Science categories.
The book uniquely covers all aspects of anaerobic biodegradation of the environmentally important hydrocarbons. The contributions by international experts cover the molecular characterization of unique biocatalysts for oxygen-independent C-H-bond functionalization, the identification of unifying concepts, and the presentation of state-of-the-art methodologies. The current knowledge of the global importance of anaerobic hydrocarbon degradation is highlighted.
Stable Isotope Probing And Related Technologies
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Author : J. Colin Murrell
language : en
Publisher: American Society for Microbiology Press
Release Date : 2010-12-10
Stable Isotope Probing And Related Technologies written by J. Colin Murrell and has been published by American Society for Microbiology Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-10 with Science categories.
The ideal starting point for investigating, developing, and implementing stable isotope technologies. • Guides researchers through basic, tested, and proven protocols including DNA, RNA, protein, and phospholipid fatty acid (PLFA) SIP, from concept and history through detailed methodology, troubleshooting, and interpretation to optimal and future uses. • Explores important and emerging applications of SIP in environmental microbiology, ranging from bioremediation and gene mining to carbon tracking and gut microflora function. • Examines explorations of further elegant isotope labeling technologies such as Raman-FISH, NanoSIMS, and isotope arrays. • Serves as a valuable resource for environmental microbiology students and researchers and genomics, biotechnology, and medical microbiology professionals.
Functional Genomics And Evolution Of Photosynthetic Systems
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Author : Robert Burnap
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-09-18
Functional Genomics And Evolution Of Photosynthetic Systems written by Robert Burnap and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-18 with Science categories.
New possibilities have been brought about by the stunning number of genomic sequences becoming available for photosynthetic organisms. This new world of whole genome sequence data spans the phyla from photosynthetic microbes to algae to higher plants. These whole genome projects are intrinsically interesting, but also inform the variety of other molecular sequence databases including the recent 'meta-genomic' sequencing efforts that analyze entire communities of organisms. As impressive as they are, are obviously only the beginning of the effort to decipher the biological meaning encoded within them. This book aims to highlight progress in this direction. This book aims toward a genome-level understanding of the structure, function, and evolution of photosynthetic systems and the advantages accrued from the availability of phyletically diverse sets of gene sequences for the major components of the photosynthetic apparatus. While not meant to be fully comprehensive in terms of the topics covered, it does provide detailed views of specific cases and thereby illustrates important new directions that are being taken in this fast-moving field—a field that involves the integration of bioinformatics, molecular biology, physiology, and ecology.
Metagenomics Methods And Protocols
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Author : Wolfgang R. Streit
language : en
Publisher: Methods in Molecular Biology
Release Date : 2018-12-14
Metagenomics Methods And Protocols written by Wolfgang R. Streit and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-12-14 with Medical categories.
Stable Isotope Probing
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Author : Marc G. Dumont
language : en
Publisher: Humana
Release Date : 2020-08-13
Stable Isotope Probing written by Marc G. Dumont and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-08-13 with Science categories.
This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.
Interactions Between Soil Particles And Microorganisms
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Author : Pan Ming Huang
language : en
Publisher: John Wiley & Sons
Release Date : 2002-03-12
Interactions Between Soil Particles And Microorganisms written by Pan Ming Huang and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-03-12 with Science categories.
Wechselwirkungen von Mineralien, organischen Verbindungen und Mikroorganismen im Boden: Dieses Handbuch wertet aktuelle Forschungsergebnisse auf diesem Gebiet kritisch aus und erläutert die Bedeutung der Zusammenhänge für Ökosysteme. Ein besonderer Schwerpunkt bildet die Wechselbeziehung zwischen Schadstoffen im Boden und Populationen von Mikroorganismen.