Characterizing Different Classes Of Focal Adhesions In Motile Cells Based On Size And Assembly Disassembly Rates
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Characterizing Different Classes Of Focal Adhesions In Motile Cells Based On Size And Assembly Disassembly Rates
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Author : Jessica Lyda
language : en
Publisher:
Release Date : 2020
Characterizing Different Classes Of Focal Adhesions In Motile Cells Based On Size And Assembly Disassembly Rates written by Jessica Lyda and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with categories.
"Cell migration is a tightly regulated process involving many proteins. Adhesions are complexes comprised of over 200 different proteins, including paxillin, that form on the cell membrane, linking the cell interior with the extracellular matrix. They act as traction points, allowing the protruding part of the cell to stabilize in its place and the rear of the cell to retract. Three classes of focal adhesions are commonly recognized in Chinese Hamster Ovary (CHO-K1) cells: nascent adhesions (NAs), focal complexes (FCs), and focal adhesions (FAs). The classes range in size, shape, location, lifetime, and dynamics. The goals of the project were to develop an image analysis pipeline to characterize adhesions across the cell, measure morphological and dynamic properties, and develop an automated adhesion classifier for NA, FC, and FA identification. The project commenced with the collection of time-lapsed images of wild-type CHO-K1 cells expressing wild-type paxillin-EGFP and proceeded by using them for the development of image analysis methods to measure adhesion properties over time. The project involved designing a MATLAB algorithm to cluster adhesions based on these measurements using K-Means. The validity of thresholds between classes was examined using cells that, at the serine 273 site of paxillin, had a phosphomimetic residue (S273D) known to induce NA formation or had an unphosphorylatable residue (S273A) known to induce FA formation. As a pharmacological control, cells were treated with Y-27632 (a chemical that inhibits ROCK, hindering maturation of adhesions) and, as a result, contained smaller, NA-appearing adhesions. The classification and verification of each class proved to be challenging as most parameter values exist on a continuum, but the careful consideration and selection of procedures in the algorithm are key components of realizing these goals"--
Approximate Analytical Methods For Solving Ordinary Differential Equations
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Author : T.S.L Radhika
language : en
Publisher: CRC Press
Release Date : 2014-10-31
Approximate Analytical Methods For Solving Ordinary Differential Equations written by T.S.L Radhika and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-10-31 with Mathematics categories.
Approximate Analytical Methods for Solving Ordinary Differential Equations (ODEs) is the first book to present all of the available approximate methods for solving ODEs, eliminating the need to wade through multiple books and articles. It covers both well-established techniques and recently developed procedures, including the classical series solution method, diverse perturbation methods, pioneering asymptotic methods, and the latest homotopy methods. The book is suitable not only for mathematicians and engineers but also for biologists, physicists, and economists. It gives a complete description of the methods without going deep into rigorous mathematical aspects. Detailed examples illustrate the application of the methods to solve real-world problems. The authors introduce the classical power series method for solving differential equations before moving on to asymptotic methods. They next show how perturbation methods are used to understand physical phenomena whose mathematical formulation involves a perturbation parameter and explain how the multiple-scale technique solves problems whose solution cannot be completely described on a single timescale. They then describe the Wentzel, Kramers, and Brillown (WKB) method that helps solve both problems that oscillate rapidly and problems that have a sudden change in the behavior of the solution function at a point in the interval. The book concludes with recent nonperturbation methods that provide solutions to a much wider class of problems and recent analytical methods based on the concept of homotopy of topology.
Molecular Biology Of The Cell
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Author : Bruce Alberts
language : en
Publisher:
Release Date : 2002
Molecular Biology Of The Cell written by Bruce Alberts and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Cytology categories.
Wound Healing
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Author : Vlad Alexandrescu
language : en
Publisher: BoD – Books on Demand
Release Date : 2016-10-12
Wound Healing written by Vlad Alexandrescu and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-12 with Medical categories.
Outstanding scientific advances over the last decades unceasingly reveal real complexity of wound-healing process, astonishing in its staged progression, as life is unfolding itself. This natural course of tissue repair seems to bear thousands of overlapping molecular and macroscopic processes that nowadays only start to unfold to our knowledge. The present volume collecting recent scientific references proposes to readers a two-folded audacious goal. First, an updated design of intimate cellular mechanisms is entailed in tissue regeneration that emanates from the first section of the book. Next, a multidisciplinary therapeutic perspective that focuses on macroscopic healing throughout the second part of this work adds clinically integrated observation. Practical diagnostic and treatment information is appended in each chapter that may equally help experienced clinicians or dedicated students and researchers in broadening essential breaking points of their work. It is the wish of all multidisciplinary experts who gather prominent author's panel of this volume to incorporate latest medical reports and compel limits of current understanding for better tissue regeneration, limb salvage, and improved quality of life of our patients.
Physics Of Cancer
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Author : Claudia Mierke
language : en
Publisher: Iph001
Release Date : 2018-10-24
Physics Of Cancer written by Claudia Mierke and has been published by Iph001 this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-24 with Science categories.
This revised second edition is improved linguistically with multiple increases of the number of figures and the inclusion of several novel chapters such as actin filaments during matrix invasion, microtubuli during migration and matrix invasion, nuclear deformability during migration and matrix invasion, and the active role of the tumor stroma in regulating cell invasion.
Rise And Fall Of Epithelial Phenotype
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Author : Pierre Savagner
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-07-05
Rise And Fall Of Epithelial Phenotype written by Pierre Savagner and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-07-05 with Science categories.
Epithelial phenotype is a dynamic stage of differentiation that can be modulated during several physiological or pathological events. The rapid conversion to a mesenchymal-like phenotype is called an epithelial-mesenchymal transition (EMT). The Rise and Fall of Epithelial Phenotype is the first book to comprehensively introduce the concept of EMT. The first part of this volume describes main examples and models and explains their physiological relevance. These examples include hydra morphogenesis, gastrulation in mouse, drosophila and sea urchin, as well as neural crest cell migration and heart morphogenesis in vertebrates. Part two reviews in detail, specific EMT molecular pathways covering extracellular induction, transduction and transcription response and modulation of cell-cell adhesion structures. It emphasizes new specific pathways with potential medical applications. EMTs can also be linked to pathological events such as wound healing and cancer progression, as detailed in this section of the book.
Molecular Biology Of The Cell
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Author :
language : en
Publisher:
Release Date : 2003
Molecular Biology Of The Cell written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Cytology categories.
Actin Based Motility
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Author : Marie-France Carlier
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-09-23
Actin Based Motility written by Marie-France Carlier and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-09-23 with Medical categories.
Since the discovery of actin by Straub in the 1950’s and the pioneering work of Oosawa on actin self-assembly in helical laments in the 1960’s, many books and conference proceedings have been published. As one of the most essential p- teins in life, essential for movement in organisms rangingfrom bacteria to higher eukaryotes, it is no surprise that actin has fascinated generations of scientists from many different elds. Actin can be considered as a “living treasure” of biology; the kinetics and thermodynamics of self-assembly, the dissipative nature of actin po- merization, the molecular interactions of monomeric and polymerized actin with regulators, the mechanical properties of actin gels, and more recently the force p- ducing motile and morphogenetic processes organized by the actin nanomachine in response to signaling, are all milestones in actin research. Discoveries that directly derive from and provide deeper insight into the fundamental properties of actin are constantly being made, making actin an ever appealing research molecule. At the same time, the explosion in new technologies and techniques in biological sciences has served to attract researchers from an expanding number of disciplines, to study actin. This book presents the latest developments of these new multiscale approaches of force and movement powered by self-assembly processes, with the hope to opening our perspectives on the many areas of actin-based motility research.
Myofibrillogenesis
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Author : Dipak K. Dube
language : en
Publisher: Springer Science & Business Media
Release Date : 2001-10-19
Myofibrillogenesis written by Dipak K. Dube and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-10-19 with Science categories.
Myofibrillogenesis has been studied extensively over the last 100 years. Until recently, we have not had a comprehensive understanding of this fundamental process. The emergence of new technologies in molecular and cellular biology, combined with classical embryology, have started to unravel some of the complexities of myofibril assembly in striated muscles. In striated muscles, the contractile proteins are arranged in a highly ordered three dimensional lattice known as the sarcomere. The assembly of a myofibril involves the precise ordering of several proteins into a linear array of sarcomeres. Multiple isoforms in many of these proteins further complicate the process, making it difficult to define the precise role of each component. This volume has been compiled as a comprehensive reference on myofibrillogenesis. In addition, the book includes reviews on myofibrillar disarray under various pathological conditions, such as familial hypertrophic cardiomyopathy (FHC), and incorporates a section on the conduction system in the heart. Much of the information in this volume has not been described elsewhere. Presented in a manner to be of value to students and teachers alike, "Myofibrillogenesis" will be an invaluable reference source for all in the fields of muscle biology and heart development.
The Extracellular Matrix An Overview
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Author : Robert Mecham
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-02-16
The Extracellular Matrix An Overview written by Robert Mecham and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-02-16 with Science categories.
Knowledge of the extracellular matrix (ECM) is essential to understand cellular differentiation, tissue development, and tissue remodeling. This volume of the series “Biology of Extracellular Matrix” provides a timely overview of the structure, regulation, and function of the major macromolecules that make up the extracellular matrix. It covers topics such as collagen types and assembly of collagen-containing suprastructures, basement membrane, fibronectin and other cell-adhesive glycoproteins, proteoglycans, microfibrils, elastin, fibulins and matricellular proteins, such as thrombospondin. It also explores the concept that ECM components together with their cell surface receptors can be viewed as intricate nano-devices that allow cells to physically organize their 3-D-environment. Further, the role of the ECM in human disease and pathogenesis is discussed as well as the use of model organisms in elucidating ECM function.