Chromatin Immunoprecipitation Assays
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Chromatin Immunoprecipitation Assays
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Author : Philippe Collas
language : en
Publisher: Humana Press
Release Date : 2009-09-02
Chromatin Immunoprecipitation Assays written by Philippe Collas and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-09-02 with Science categories.
Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cell’s biology.
Applications Of The Chromatin Immunoprecipitation Assay For Studying Mechanisms Of The Receptor Mediated Transcription
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Author : Shengxi Liu
language : en
Publisher:
Release Date : 2006
Applications Of The Chromatin Immunoprecipitation Assay For Studying Mechanisms Of The Receptor Mediated Transcription written by Shengxi Liu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.
Chromatin Immunoprecipitation Assays
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Author : Philippe Collas
language : en
Publisher: Methods in Molecular Biology
Release Date : 2009-08-25
Chromatin Immunoprecipitation Assays written by Philippe Collas and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-08-25 with Computers categories.
In this book, researchers deeply involved in the development and improvement of chromatin immunoprecipitation assays (ChIP) provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects.
Chromatin Immunoprecipitation
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Author : Neus Visa
language : en
Publisher: Humana Press
Release Date : 2017-10-14
Chromatin Immunoprecipitation written by Neus Visa and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-10-14 with Science categories.
This up-to-date volume includes protocols that illustrate the broad use of chromatin immunoprecipitation (ChIP) and ChIP-related methods in a variety of biological research areas. The collection also includes protocols designed to improve the performance of ChIP for specific applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chromatin Immunoprecipitation: Methods and Protocols features techniques, including bioinformatic analysis of ChIP data, will be of interest to a very broad research community in the fields of biochemistry, molecular biology, microbiology, and biomedicine.
Immunology Overview And Laboratory Manual
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Author : Tobili Y. Sam-Yellowe
language : en
Publisher: Springer Nature
Release Date : 2021-08-01
Immunology Overview And Laboratory Manual written by Tobili Y. Sam-Yellowe and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-08-01 with Medical categories.
A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.
Epigenetics Methods
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Author : Trygve Tollefsbol
language : en
Publisher: Academic Press
Release Date : 2020-07-08
Epigenetics Methods written by Trygve Tollefsbol and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-08 with Medical categories.
In recent years, the field of epigenetics has grown significantly, driving new understanding of human developmental processes and disease expression, as well as advances in diagnostics and therapeutics. As the field of epigenetics continues to grow, methods and technologies have multiplied, resulting in a wide range of approaches and tools researchers might employ. Epigenetics Methods offers comprehensive instruction in methods, protocols, and experimental approaches applied in field of epigenetics. Here, across thirty-five chapters, specialists offer step-by-step overviews of methods used to study various epigenetic mechanisms, as employed in basic and translational research. Leading the reader from fundamental to more advanced methods, the book begins with thorough instruction in DNA methylation techniques and gene or locus-specific methylation analyses, followed by histone modification methods, chromatin evaluation, enzyme analyses of histone methylation, and studies of non-coding RNAs as epigenetic modulators. Recently developed techniques and technologies discussed include single-cell epigenomics, epigenetic editing, computational epigenetics, systems biology epigenetic methods, and forensic epigenetic approaches. Epigenetics methods currently in-development, and their implication for future research, are also considered in-depth. In addition, as with the wider life sciences, reproducibility across experiments, labs, and subdisciplines is a growing issue for epigenetics researchers. This volume provides consensus-driven methods instruction and overviews. Tollefsbol and contributing authors survey the range of existing methods; identify best practices, common themes, and challenges; and bring unity of approach to a diverse and ever-evolving field. Includes contributions by leading international investigators involved in epigenetic research and clinical and therapeutic application Integrates technology and translation with fundamental chapters on epigenetics methods, as well as chapters on more novel and advanced epigenetics methods Written at verbal and technical levels that can be understood by scientists and students alike Includes chapters on state-of-the-art techniques such as single-cell epigenomics, use of CRISPR/Cas9 for epigenetic editing, and epigenetics methods applied to forensics
Transcriptional Regulation
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Author : Ales Vancura
language : en
Publisher: Humana
Release Date : 2016-08-23
Transcriptional Regulation written by Ales Vancura and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
Through many recent remarkable developments, perhaps the most significant advancements in the study of transcriptional regulation are the development of genome-wide approaches for measuring gene expression, exemplified by gene chips (chip), and chromatin immunoprecipitation assays (ChIP) for measuring in vivo protein-DNA interactions at any genomic loci. Transcriptional Regulation: Methods and Protocols takes this progress and builds upon it with a collection of key protocols used in expert laboratories around the world. Divided into four convenient sections, this detailed volume explores promoter elements, transcription factors, and preinitiation complex (PIC) assembly, chromatin structure, chromatin modifying complexes, and RNA synthesis and regulation. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Comprehensive and accessible, Transcriptional Regulation: Methods and Protocols equally serves senior researchers and scientists experienced in transcriptional regulation as well as graduate students and scientists who wish to study transcriptional regulation for the first time.
Advances In International Rice Research
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Author : Jin Quan Li
language : en
Publisher: BoD – Books on Demand
Release Date : 2017-03-15
Advances In International Rice Research written by Jin Quan Li and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-03-15 with Technology & Engineering categories.
Rice provides staple food for more than 50% of the world's population and is an important crop in the world. With the new technologies such as high-throughput genome sequencing and integrated "-omis" methods applied in rice researches, great advancements have been made. This book was aimed to show a glance of new advancements in the international rice researches. The first section of the book introduced rice cultivation and production. As core sections of the book, the second and third sections introduced physiological and genetic mechanisms on grain quality and biotic and abiotic stress resistance as well as breeding. In the last section, we introduced new technologies such as chromatin immunoprecipitation, integrated "-omis" methods, and bistatic interferometry technology in rice research.
Reproducibility And Reliability Of Chromatin Immunoprecipitation In Clinical Research
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Author : Kelli McDonough
language : en
Publisher:
Release Date : 2016
Reproducibility And Reliability Of Chromatin Immunoprecipitation In Clinical Research written by Kelli McDonough and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with DNA. categories.
Association between histones and DNA is crucial for many cellular functions such as gene transcription and epigenetic silencing. Changes to chromatin structure influence gene expression via histone modifications. Chromatin immunoprecipitation (ChIP) is an experimental technique used to investigate the interactions between histones and DNA. ChIP determines the specific location in the genome that histone modifications are associated with, indicating the target of the histone modifiers. Despite the appeal of ChIP as an in vitro technique, there are limitations to its use. There is variability from one preparation to the next, given the many steps and reagents used throughout the technique. No one has been able to demonstrate consistent results in a clinical population using white blood cells. It was established, for the first time, the normal variability of ChIP results in THP-1 cells, Jurkat cells, TF-1a, and human male circulating leukocytes over time for histone modifications H3k4me3 and H3k9Ac.
High Throughput Genomic Assays
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Author : Kasey Robert Hutt
language : en
Publisher:
Release Date : 2009
High Throughput Genomic Assays written by Kasey Robert Hutt and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with categories.
Determining DNA sequence has been a principle tool for several methods in biology research. From whole genome sequencing to RNA expression assays to several types of immunoprecipitation experiments, sequencing DNA has been a staple detection technique. Recent advances in sequencing and detection of DNA has revealed many new possibilities, and problems, with regards to data analysis. Here, I present a study analyzing a novel detection technology and sequencing method. These are both important contributions for not only providing new insights for utilizing a more sensitive detection technique, but also creating a method which enables any researcher to quickly analyze the unheralded amount of sequence data now being produced, soon be available to everyone. Chapter 2 focuses on the current uses and analysis of a novel DNA detection technique called DNA Selection and Ligation (DSL). By taking advantage of the more sensitive and specific DSL strategy, any assay that is dependent on DNA detection is improved. In this chapter, I show how DSL can be used to modify the standard chromatin immunoprecipitation (ChIP)-on-chip assay (termed ChIP-DSL), in both the promoter-specific and tiling cases. Additionally, I show how the ChIP-DSL method gives promising results for a high-throughput version of the chromatin conformation capture (3C) assay, which is used to measure if two regions of DNA are interacting. Chapter 3 concentrates on next-generation sequencing, more specifically on the Illumina Genome Analyzer (GA). After describing the details of how the sequencing is performed and analyzed, I discuss the current flaw in these datasets, and propose a solution to the problem, the Genome Ontology. I then give several examples where the Genome Ontology is helpful in extracting knowledge from these incredibly large datasets. Chapter 4 describes numerous future directions of my own work as well as several of my observations that resulted from working with these next-generation sequencing datasets.
