Cryopreservation Of Fish Eggs And Sperm
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Cryopreservation Of Fish Eggs And Sperm
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Author : Jorma Piironen
language : en
Publisher: Nordic Council of Ministers
Release Date : 1993
Cryopreservation Of Fish Eggs And Sperm written by Jorma Piironen and has been published by Nordic Council of Ministers this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with categories.
Cryopreservation Of Fish Gametes
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Author : Judith Betsy
language : en
Publisher: Springer Nature
Release Date : 2020-06-27
Cryopreservation Of Fish Gametes written by Judith Betsy and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-06-27 with Science categories.
Understanding the reproductive physiology and endocrinology of fishes is essential for captive maturation and seed production in the field of aquaculture. Studying the spermatology of fishes is a comparatively new focus in aquaculture, which has emerged as an important area of fish research over the past two decades. In this regard, the cryopreservation of fish gametes is a crucial aspect. Moreover, energetics studies of gametes have become essential, considering the loss of vigour in the spermatozoa after cryopreservation.The latest development in this context is the cryopreservation of spermatogonial stem cell, which is also covered in the book, along with detailed information on embryo cryopreservation in fishes and crustaceans. The role of cryopreservation in conservation programmes is another important aspect, one that will especially interest biologists.This book addresses central issues in fish gamete cryopreservation and breeding, while also reviewing the history of cryopreservation. Its most unique feature is the breadth of its coverage, from basic information on reproduction in fishes, to such advanced topics as embryo cryopreservation. Chiefly intended as a handy troubleshooting guide, the book represents a valuable resource for research students in related fields.
Fertilization Of Salmonid Eggs With Cryopreserved Sperm
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Author : A. G. Ott
language : en
Publisher:
Release Date : 1970
Fertilization Of Salmonid Eggs With Cryopreserved Sperm written by A. G. Ott and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1970 with Spermatozoa categories.
Numerous fishery researchers have attempted to preserve viable fish sperm, but only three investigations were successful in the fertilization of fresh eggs with frozen and thawed sperm: Blaxter (1953); Hoyle and Idler (1968); and Graybill and Horton (1969). Significant advances reported in this thesis were: the development of suitable life extenders for semen of steelhead trout (Salmo gairdneri), and coho (Oncorhynchus kisutch) and Chinook (0. tshawytscha) salmon; the selection of a life protector composed of 8.0% dimethyl. sulfoxide; the verification that the best rate of freezing semen was -30 C/mm, arid that the best method of thawing frozen sperm was in a 4 C water bath; and the determination that no equilibration time was necessary when fresh semen was diluted with extenders and protectors. Application of the above techniques led to the successful cryo-preservation of male gametes of steelhead trout and Chinook and coho salmon in liquid nitrogen ( -196 C). Frozen and thawed sperm was mixed with fresh eggs and resulted in maximum fertilizations of 59.1% for steelhead trout, 38.4% for spring Chinook salmon, and 79.2% for coho salmon. Details of the chemical constituents of the extenders and protectors tested are appended.
Steps Towards Successful Cryopreservation Of Fish Gametes
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Author : Megan C. Chastain
language : en
Publisher:
Release Date : 2014
Steps Towards Successful Cryopreservation Of Fish Gametes written by Megan C. Chastain and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with Cryopreservation of organs, tissues, etc categories.
"Cryopreservation is a powerful tool for the long term storage of genetic material from endangered species. While mammalian gametes have long been freezable, eggs and embryos from fish have yet to be successfully frozen. Furthermore, protocols for freezing fish sperm have low success rates. In an effort to overcome the limitations in gamete manipulation currently impeding successful fish gamete cryopreservation, I outline two experiment using zebrafish (Danio rerio) as a model for freshwater fish species. First, I attempted to extend working time in both eggs and sperm by incubating the gametes in rainbow trout oviductal fluid (RTOF), RTOF + HEPES, or RTOF under a blood-gas atmosphere. Incubation in RTOF under a blood gas atmosphere significantly extended viability in both gametes to 24 hours. Second, I attempted to minimize the number of sperm necessary to fertilize a single egg by reducing the volume in which the eggs were fertilized and by comparing the traditional fertilization system to a novel inverted drop technique. There were no differences between fertilization rates with sperm concentrations ranging from 1575 to 4 sperm/[micro]L . Furthermore, the inverted drop technique improved overall fertilization. Both increased gamete manipulation time and reduced sperm waste have the potential to improve cryopreservation success in fish gametes"--P. v.
Cryopreservation Of Salmonid Sperm
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Author : Joseph G. Cloud
language : en
Publisher:
Release Date : 1997
Cryopreservation Of Salmonid Sperm written by Joseph G. Cloud and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Salmonidae categories.
Methods In Reproductive Aquaculture
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Author : Elsa Cabrita
language : en
Publisher: CRC Press
Release Date : 2008-08-22
Methods In Reproductive Aquaculture written by Elsa Cabrita and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-08-22 with Nature categories.
The large amount of information on fish reproduction available is not always readily accessible to all interested parties. Written to appeal to aquaculturalists, conservation managers, and scientific researchers, Methods in Reproductive Aquaculture provides an overview of available techniques and addresses ways to improve depleted stocks of endange
Cryopreservation Of Fish Gametes
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Author :
language : en
Publisher:
Release Date : 2020
Cryopreservation Of Fish Gametes written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with Electronic books categories.
Understanding the reproductive physiology and endocrinology of fishes is essential for captive maturation and seed production in the field of aquaculture. Studying the spermatology of fishes is a comparatively new focus in aquaculture, which has emerged as an important area of fish research over the past two decades. In this regard, the cryopreservation of fish gametes is a crucial aspect. Moreover, energetics studies of gametes have become essential, considering the loss of vigour in the spermatozoa after cryopreservation. The latest development in this context is the cryopreservation of spermatogonial stem cell, which is also covered in the book, along with detailed information on embryo cryopreservation in fishes and crustaceans. The role of cryopreservation in conservation programmes is another important aspect, one that will especially interest biologists. This book addresses central issues in fish gamete cryopreservation and breeding, while also reviewing the history of cryopreservation. Its most unique feature is the breadth of its coverage, from basic information on reproduction in fishes, to such advanced topics as embryo cryopreservation. Chiefly intended as a handy troubleshooting guide, the book represents a valuable resource for research students in related fields.
Cryopreservation Of Rohu Fish Sperm
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Author : Md. Sainur Samad
language : en
Publisher:
Release Date : 2012
Cryopreservation Of Rohu Fish Sperm written by Md. Sainur Samad and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.
Cryo Preservation Of Viable Fish Sperm
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Author : James Roger Graybill
language : en
Publisher:
Release Date : 1968
Cryo Preservation Of Viable Fish Sperm written by James Roger Graybill and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1968 with Fish culture categories.
Fishery researchers have attempted to preserve viable fish sperm for extended periods of time. Only limited success has been achieved with storage of spermatozoa from salmonids. The principal accomplishments of this investigation during the fiscal year 1966-1967 were the development of standard methods for the collection and evaluation of fish sperm, the development of an extender and life protector of spermatozoa, and the development of a freezing and thawing procedure for the live preservation of fish sperm at temperatures of liquid nitrogen ( -196 C). Collection of fish sperm was accomplished by means of a suction device with which semen was withdrawn from the genital pore of a fish into a test tube. A modification of the conventional hemocytometric technique was used to determine the concentration of sperm in fish semen. The use of vital stains to differentiate between live and dead sperm cells was investigated and abandoned due to inconsistent results. A subjective method of estimating the viability and relative motility of spermatozoa was established. Experiments were conducted to prolong the viability of sperm cells in the unfrozen state. The deleterious effect of water on prolonging the viability of fish sperm was confirmed. I found that a high percentage of sperm cells remained viable for at least three days if they were refrigerated under aerobic conditions and kept from contact with water. Increased dilution of fish semen with extenders reduced the viability of sperm cells. The freezing point depression of solutions isotonic to fish spermatozoa was found to be -0.51 C. My evidence suggests that the key to maintaining viable fish sperm at 5 C is to provide substances in the extender which can be aerobically metabolized and stored as latent energy by the spermatozoa. Experiments were conducted to prolong the viability of fish spermatozoa in the frozen state. Dimethyl sulfoxide (DMS0) gave the best results of the life protectors studied. Equilibration of the sperm cells in increasing concentrations of a life protector may have increased the viability of the spermatozoa. During several experiments, the optimum concentration of protector increased as the rate of freezing increased. The best survival of viable sperm was obtained in samples that were frozen in Solution 48 in combination with DMSO as a protector. Thawing the ampoules of semen in a 4 C water bath yielded the highest recovery of viable spermatozoa. Alevins were produced from eggs fertilized with cryogenically preserved fish sperm. These alevins appeared to be as normal as young fish produced from unfrozen spermatozoa. The fertility rate was low (0-18%), but to my knowledge this is the first successful attempt to fertilize salmonid eggs with cryo-preserved spermatozoa. The findings in this study warrant further investigation.
Cryopreservation In Aquatic Species
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Author : Terrence R. Tiersch
language : en
Publisher:
Release Date : 2000
Cryopreservation In Aquatic Species written by Terrence R. Tiersch and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Aquatic animals categories.
