Cryopreservation Of Plant Cells And Organs
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Cryopreservation Of Plant Cells And Organs
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Author : Kartha
language : en
Publisher: CRC Press
Release Date : 1985-04-03
Cryopreservation Of Plant Cells And Organs written by Kartha and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985-04-03 with Science categories.
Plant cell culture. Basic principles pf freezing injury to plant cells: natural tolerance and approaches to cryopreservation. Cryobiology of isolated protoplasts: application to plant cell cryopreservation. Biophysical and ultrastructural studies of membrane alterations in plant cells during extracellular freezing: molecular mechanisms of membrane injury. Cryoprotective compounds in the viable freezing of plant tissues. Meristem culture and germplasm preservation. Cryopreservation of shoot-tips of fruit trees and herbaceous plants. Cryopreservation of potato meristems...
Cryopreservation Of Plant Cells And Organs
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Author : K. K. Kartha
language : en
Publisher:
Release Date : 1981
Cryopreservation Of Plant Cells And Organs written by K. K. Kartha and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1981 with categories.
Genetic Preservation Of Plant Cells In Vitro
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Author : Brian Grout
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Genetic Preservation Of Plant Cells In Vitro written by Brian Grout and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The long-term storage and maintenance of viable plant cells and organs is an area of active concern across the range of pure and applied plant sciences. In academic, government and commercial laboratories, the extended storage of propagules of one sort or another, with maximum protection of the genome from mutation and altered expression, is often a very necessary activity that can draw heavily on resources and effort. However, preservation per se is typically not an activity in its own right, but a facilitating technology that is part of a larger programme of work. Consequently, there are many laboratories that do not have the benefit of a specialist in storage technology, and have to delegate the responsibility to individuals, or teams, who are faced with a daunting learning curve. To maximise the chances of success, in the shortest possible time and with minimum losses, these researchers need sources of reference that are au thoritative and soundly based in practical experience.
Cryopreservation Of Plant Germplasm I
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Author : Y. P. S. Bajaj
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-09
Cryopreservation Of Plant Germplasm I written by Y. P. S. Bajaj and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-09 with Technology & Engineering categories.
The germ plasm of numerous plant species, especially those of forest trees, some agricultural crops, and medicinal plants, is endangered and threatened with extinction. This depletion of germplasm pools and the shrinkage of naturally occurring genetic resources have caused international concern. Conventionally, the germplasm of plants is conserved through seeds, tubers, roots, corms, rhizomes, bulbs, cuttings, etc. However, the germ plasm of a number of trees and plantation crops (such as coconut, cocao, coffee, oil palm, rubber, mango, horse chestnut, etc. ) cannot be preserved since their seed are short-lived (recalcitrant). Likewise, germplasm of vegetatively propagated crops (such as potato and cassava) cannot be stored on a long term basis and has to be grown and multiplied periodically in nurseries and fields. The plants are thus exposed to unpredictable weather conditions and diseases, with the result that instances are known where entire genetic stocks are lost. Therefore, unconventional methods are being developed for the storage and international exchange of germplasm. For this purpose in vitro cultures have been employed, but they can only enable short-to medium term preservation; moreover, cell cultures upon repeated subculture undergo genetic erosion. In view of the recent developments in the in vitro induction of genetic variability through somaclonal variation, somatic hybridization, recombinant DNA technology, etc. , new methods need to be employed for the storage of desirable cultures. In this regard freeze preservation of cells in liquid nitrogen (-196 0q, like that of semen, enables long-term storage, theoretically, for an indefinite period of time.
Plant Cryopreservation
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Author : Carla Benelli
language : en
Publisher: Mdpi AG
Release Date : 2022-01-05
Plant Cryopreservation written by Carla Benelli and has been published by Mdpi AG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-05 with Science categories.
Cryopreservation is the storage of cells, tissues and organs at ultra-low temperatures (-196 °C) and can be an important tool for long-term conservation of plant genetic resources. A wide range of cryogenic procedures have been developed for in vitro explants, dormant buds and non-orthodox seeds in many plant species; however, research that provides novelty, insight and advances for developing and improving new protocols in plant species is still needed. This book includes the development of new protocols, physical and chemical studies related to preservation in liquid nitrogen, and some reviews on several aspects of cryopreservation. This publication will be a useful document for the community of cryopreservation scientists.
Plant Cryopreservation A Practical Guide
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Author : Barbara B.M. Reed
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-31
Plant Cryopreservation A Practical Guide written by Barbara B.M. Reed and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-31 with Technology & Engineering categories.
Cryopreservation has proven to be an important tool for the storage and conservation of plant genetic resources. This book is a unique resource for plant scientists, providing more than 100 ready-to-use cryopreservation protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems and for cell types such as suspension and callus cultures.
Cryopreservation Of Plant Germplasm I
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Author : Y. P. S. Bajaj
language : en
Publisher: Springer Verlag
Release Date : 1995
Cryopreservation Of Plant Germplasm I written by Y. P. S. Bajaj and has been published by Springer Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with Science categories.
Whole plants have been regenerated from isolated protoplasts, cells, tissues, and organs freeze-preserved in liquid nitrogen. Thus cryopreservation has proved to be an important tool for the storage and conservation of plant genetic resources. Numerous experts have contributed to this volume, which is divided into the following sections: General introduction into the cryopreservation of plant cells, tissues, organs and seeds; trees; cereals and grasses; legumes and oilseed crops; fruits, vegetables, tuber and root crops; medicinal, aromatic and ornamental plants.
Cryopreservation Of Plant Germplasm Ii
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Author : L.E. Towill
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-14
Cryopreservation Of Plant Germplasm Ii written by L.E. Towill and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-14 with Science categories.
Ex situ preservation of germplasm for higher plant species has been accom plished using either seeds or clones, but storage of these under typical condi tions does not provide the extreme longevities that are needed to minimize risk of loss. Costs of maintenance and regeneration of stocks are also high. Systems that provide virtually indefinite storage should supplement existing methods and it is within this context that cryopreservation is presented. The use of low temperature preservation was initially more a concern of medicine and animal breeding, and was expanded to plants in the 1970s. Sur vival after cryogenic exposure has now been demonstrated for diverse plant groups including algae, bryophytes, fungi and higher plants. If survival is com monplace, then the eventual application is a cryopreservation system, whereby cells, tissues and organs are held indefinitely for use, often in the unforeseen future. The increasing interest and capabilities for application could not have occurred at a more opportune time since expanding human populations have placed unprecedented pressures on plant diversity. This book emphasizes cry opreservation of higher plants and was initially driven by the concern for loss of diversity in crops and the recognized need that this diversity would be essential for continued improvement of the many plants used by society for food, health and shelter. The interest in cryopreservation has been expanded by conservationists and their concerns for retaining, as much as possible, the diversity of natural populations. The need for cryopreservation, thus, is well established.
Plant Cell Culture
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Author : Robert A. Gonzales
language : en
Publisher:
Release Date : 1994
Plant Cell Culture written by Robert A. Gonzales and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with categories.
Initiation and maintenance of callus and cell suspension cultures; Isolation, culture, and regeneration of protoplasts; Applications of protoplast technology; Selection of plant cells for desirable characteristics; Plant regeneration via embryogenic suspension cultures; Applied aspects of plant regeneration; Cryopreservation; Secondary products from cultured cells and organs: molecular and cellular approaches; Large scale culture.
Cryopreservation And Freeze Drying Protocols
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Author : John G. Day
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Cryopreservation And Freeze Drying Protocols written by John G. Day and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
The storage of biological material for regular or future use is a fundamental requirement in many biological and medical sciences. Cryopreservation and freeze-drying are the preferred techniques for achieving long-term storage, and have been applied to a diverse range of biological materials. Though the basis for many methodologies is common, laboratories frequently lack expertise with the correct storage procedures, so that many apply outdated or inappropriate protocols for storing their samples or cultures. Cryopreservation and Freeze-Drying Protocols is a compilation of the many and varied methodologies that have been developed in expert laboratories. The protocols are reproducible, robust, and in most instances have been transferred quite successfully to other laboratories. Our intended readers are those proposing to establish or improve biostorage systems in their own laboratories or units, whether concerned with culture collections, animal husbandry, aquaculture, or human fertilization programs. Because the emphasis of Cryopreservation and Freeze-Drying Protocols is on methodology, it is our intention to provide readers with the tools to make practical progress without reference to other sources. Each chapter deals with an organelle, cell, or tissue type: a short int- duction on the status of its biostorage development is followed by a detailed description of the materials required and a methodological p- tocol to be followed, with explanatory notes. This is very much a first edition; we hope and trust that future editions will contain cryopreservation and freeze-drying protocols for ceils, tissues, and organs that are at present still recalcitrant to succe- ful preservation.