Cytokine Bioassays
DOWNLOAD
Download Cytokine Bioassays PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Cytokine Bioassays book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Cytokine Bioassays
DOWNLOAD
Author : Ivana Vancurova
language : en
Publisher: Humana
Release Date : 2016-08-23
Cytokine Bioassays written by Ivana Vancurova and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Medical categories.
Cytokines are pleiotropic regulatory proteins involved in essentially all biological processes and associated with a wide variety of diseases, including inflammatory disorders as well as many types of cancer and leukemia. Knowledge about the quantitative and qualitative nature of cytokine production is critical in the understanding of normal and pathological processes. The cytokine detection in biological and clinical samples faces many challenges including their low abundance, the need to distinguish between active and latent cytokine forms, and the need to measure multiple cytokines in a single assay. This volume will provide a comprehensive collection of classic and cutting-edge methodologies that are currently used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. The chapters are divided into four main categories. The first group focuses on the immunodetection of released cytokines in tissue culture supernatants, plasma, serum and whole blood samples by immunoassays. These immunoassays measure the total concentrations of released cytokines regardless of their biological activity and include ELISA, flow cytometry, ELISPOT and the antibody-based proximity ligation. The second group will focus on the analysis of biologically active cytokines by bioassays using neutralizing antibodies, chemotaxis assay, cytokine-induced cell degranulation assay, cell proliferation and differentiation, cytokine-induced cytokine production and the radioreceptor cytokine assay. The third group focuses on the analysis of intracellular cytokines by flow cytometry, western blotting and fluorescence and confocal microscopy. In addition, this category includes protocols for quantitative analysis of cytokine gene expression by real time RT-PCR and analysis of the cytokine promoter occupancy by chromatin immunoprecipitation. The fourth group focuses on the recently developed multiplex arrays that can measure multiple cytokines in the same sample at the same time. This group includes quantification of multiple cytokines using cytometric bead arrays, ELISPOT assays, proteomics cytokine evaluation, multiplexed proximity ligation assays for high-throughput cytokine analysis and finally, cytokine gene expression analysis by gene arrays. The protocols will be written by experienced basic and clinical researchers with hands-on knowledge of the described protocols. By covering a broad variety of methods used in cytokine detection and analysis, this book will be of interest not only to biochemists, molecular biologists and immunologists but also to physician-scientists working in the field of cytokine research.
Cellular Cytokine Bioassays In Interleukin 1 Quantitation
DOWNLOAD
Author : Lars Remvig
language : en
Publisher:
Release Date : 1992
Cellular Cytokine Bioassays In Interleukin 1 Quantitation written by Lars Remvig and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with categories.
Cellular Cytokine Bioassays In Interleukine 1 Quantitation
DOWNLOAD
Author : Lars Remvig
language : en
Publisher:
Release Date : 1992
Cellular Cytokine Bioassays In Interleukine 1 Quantitation written by Lars Remvig and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with categories.
Guidebook To Cytokines And Their Receptors
DOWNLOAD
Author : Nicos Nicola
language : en
Publisher: Sambrook and Tooze Publications
Release Date : 1994
Guidebook To Cytokines And Their Receptors written by Nicos Nicola and has been published by Sambrook and Tooze Publications this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Cellular immunity categories.
The number of cytokines and related growth factors has increased dramatically in recent years to several dozen. This text provides a succinct reference manual for all known cytokines, growth factors, interleukins and lymphokines as well as their receptors.
Cytokine Cell Biology
DOWNLOAD
Author : Fran Balkwill
language : en
Publisher: OUP Oxford
Release Date : 2000-11-09
Cytokine Cell Biology written by Fran Balkwill and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-11-09 with Science categories.
Cytokine Cellular Biology focuses on cell biology techniques for studying cytokines, cytokine receptors, and cytokine driven processes. Assays for human B cell responses, leucocyte migration, haematopoietic growth factors, macrophage activation by cytokines, RIA, IRMA, and ELISA assays, and quantitative biological assays for cytokines are all covered in detail. There are also updated chapters on studying cytokine regulation of endothelial cells; the measurement of proliferative, cytostatic, and cytolytic activity of cytokines; and the development of antibodies to cytokines. In addition there is a new chapter on the use of flow ctyometry and intracellular fluorescent staining. Written by experts in the field, Cytokine Molecular Biology and Cytokine Cellular Biology form a comprehensive and essential guide to cytokine research.
Biological Characterization And Assay Of Cytokines And Growth Factors
DOWNLOAD
Author : Fred Brown
language : en
Publisher: S. Karger AG (Switzerland)
Release Date : 1999
Biological Characterization And Assay Of Cytokines And Growth Factors written by Fred Brown and has been published by S. Karger AG (Switzerland) this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Computers categories.
Cytokines and growth factors have vast therapeutic potentials for a wide range of diseases. Many have been cloned and produced, using recombinant DNA technology, by the biopharmaceutical industry. Of these, several have proved to be extremely successful biological medicines with increasing numbers undergoing clinical trials. One aspect of the successful development of these proteins as therapeutics is assuring quality control. This can mainly be achieved through thorough characterization of the product, both biologically and physico-chemically. In this book, leading scientists from industry, research and regulatory agencies present papers on the various methods currently available for analyzing cytokines and growth factors, including novel, developing technology. A particular focus on bioassays provides a unique and comprehensive coverage of their relevance, design, execution and analysis during product development and manufacture. This volume should be read by all those working on protein therapeutics, as many of the principles described generally apply to any biological product. Scientists working in industry and regulatory authorities will find it a useful reference for drug development and licensing.
The Cytokine Factsbook And Webfacts
DOWNLOAD
Author : Katherine A. Fitzgerald
language : en
Publisher: Academic Press
Release Date : 2001-10-09
The Cytokine Factsbook And Webfacts written by Katherine A. Fitzgerald and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-10-09 with Medical categories.
Completely revised and expanded, this second edition of The Cytokine FactsBook is the most up-to-date reference manual available for all current well-characterized interleukins, cytokines, and their receptors. An additional 52 cytokines are included, doubling the number of entries from the previous edition. The key properties of each cytokine are described and presented in a very accessible format with diagrams for each of the receptors. The Cytokine FactsBook includes free online access to the regularly updated Cytokine Webfacts. Cytokine Webfacts is a web-based comprehensive compendium of facts about cytokines and their receptors that includes a variety of data representations, such as text, signal pathway diagrams and 3D images. This exciting resource is integrated into other databases via hypertext links to provide a unique network, and contains a web-enabled version of RasMol for viewing structures.
Cytokines
DOWNLOAD
Author : Frances R. Balkwill
language : en
Publisher: Oxford University Press, USA
Release Date : 1991
Cytokines written by Frances R. Balkwill and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with Language Arts & Disciplines categories.
Provides a catalogue of techniques that are applicable to cytokine research. The book selects a number of biological models and outlines the ways of assaying cykotine activity in these systems. Also covered is cykotine production at the level of messenger RNA and protein.
Cytokine Protocols
DOWNLOAD
Author : Marc De Ley
language : en
Publisher: Humana Press
Release Date : 2011-11-18
Cytokine Protocols written by Marc De Ley and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-11-18 with Science categories.
“Recent studies have discovered new known and characterized cytokines, allowing for advancement in miniaturization of micro-analytical methods as well as the extensive development of bio-informatics and nanotechnology. These advancements have allowed researchers to reduces sample sizes making for more accurate determinations then previously possible. In Cytokine Protocols: Second Edition, expert researchers in the field detail many of the methods which are now commonly used to study cytokines. These methods and techniques for studying cytokines include historical importance and the importance of researchers using bioassay, quantification, and characterization of cytokine related RNAs, posttranscriptional modifications of RNA, either naturally or artificially, and observations at the protein level. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Authoritative and practical, Cytokine Protocols: Second Edition seeks to aid scientists in furthering the crucially important advancement of cytokine research.”
The Cytokine Factsbook
DOWNLOAD
Author : Robin E. Callard
language : en
Publisher:
Release Date : 1994
The Cytokine Factsbook written by Robin E. Callard and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.
How do you keep track of basic information on the proteins you work with? Where do you find details of their physicochemical properties, amino acid sequences, gene organization? Are you tired of scanning review articles, primary papers and databases to locate that elusive fact? The Academic Press FactsBook series will satisfy scientists and clinical researchers suffering from information overload. Each volume provides a catalogue of the essential properties of families of molecules. Gene organization, amino acid sequences, physicochemical properties, and biological activity are presented using a common, easy-to-follow format. Taken together they compile everything you wanted to know about proteins but were too busy to look for. More than 45 entries on human and murine cytokines and their receptors, including: * Cytokine receptor superfamilies * Interleukins 1-15 * Other cytokines * Additional information is provided on: * Alternative nomenclature * Cross reactivity * Sources * Bio assays * Physicochemical properties of the cytokines and their receptors * 3-D structure * Gene structure and chromosomal localisation * Amino acid sequences of the cytokines and their receptors * Receptors * Receptor distribution * Signal transduction * Chromosomal location of the receptors * References