Diffusion Chamber Culture
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Diffusion Chamber Culture
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Author : E. P. Cronkite
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Diffusion Chamber Culture written by E. P. Cronkite and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
Even though diffusion chamber culture was commenced long before ortho dox tissue culture by Metchnikoff (1887) there have been only sporadic attempts to use this methodology to study cell proliferation (review by Carsten, Chap. 1). Not so long ago diffusion chamber culture was nicknamed "confusion chamber" culture. I believe this conference has removed the confusion and will truly point out the intrinsic value of the system. It is not a substitute for established in vitro culture nor for in vivo studies. It complements both. Dr. Arne B~yum introduced diffusion chamber culture at Brookhaven National Laboratory. After some modest success in showing that one could culture human bone marrow and with appropriate stimuli induce erythro poiesis in diffusion chambers, several of the participants at this conference visited Brookhaven to learn firsthand this simple technology and to apply it in their own laboratories. However, the technique did not spread widely and controversy arose in which the same question was repeatedly asked: Can the diffusion chamber technique provide information that is not ob tainable more rapidly and easily, and at less expense by the in vitro tech niques? As a result of our deep interest in and involvement with diffusion chamber culture Dr. A. L. Carsten and I organized this conference. A major objective of this conference was to seek answers to the above question.
History And Overview Of The In Vivo Diffusion Chamber D C Culture System
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Author :
language : en
Publisher:
Release Date : 1979
History And Overview Of The In Vivo Diffusion Chamber D C Culture System written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1979 with categories.
The development of the modern diffusion chamber system has been traced from the nearly 100 year old concept for in vivo growth of isolated cells in a semi-permeable container to the modern systems consisting of single and multiple chambers with differing wall materials. Various applications of the system and methods of analysis are discussed. The many advantages and disadvantages of this system are considered as compared with other available culture systems.
Introduction For Diffusion Chamber Culture Symposium
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Author :
language : en
Publisher:
Release Date : 1979
Introduction For Diffusion Chamber Culture Symposium written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1979 with categories.
The diffusion-chamber system has been applied to studies of cell kinetics, progenitor cell quantitation, humoral effects, immunological effects, cytogenetics, organogenesis, and the cellular effects of drugs and physical factors such as radiation, hypoxia, etc. Chamber contents have been analyzed by clot dissolution with measuring of cell content, limiting dilution evaluation, radionuclide utilization (tritiated thymidine labeling), growth of colony number, size and type, CFU-S or CFU-C content, or proliferation by secondary culture in mice or in vitro systems, and chromosome changes. Cell types ranging from embryonal tissues to adult normal and neoplastic tissues have been grown in hosts across species barriers. Advantages and disadvantages of this system are discussed.
Diffusion Chamber Culture Hemopoiesis Cloning Of Tumors Cytogenetic And Carbinogenic Assays
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Author : E.P. Cronkite
language : en
Publisher:
Release Date : 1980
Diffusion Chamber Culture Hemopoiesis Cloning Of Tumors Cytogenetic And Carbinogenic Assays written by E.P. Cronkite and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1980 with categories.
Use Of Diffusion Chamber Cultures In The Study Of Normal And Leukemic Cell Proliferation In Man
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Author :
language : en
Publisher:
Release Date : 1973
Use Of Diffusion Chamber Cultures In The Study Of Normal And Leukemic Cell Proliferation In Man written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1973 with categories.
Diffusion Chamber Culture Of Hamster Melanoma Cells In Vivo
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Author : Georg Gerhard Schieferstein
language : en
Publisher:
Release Date : 1974
Diffusion Chamber Culture Of Hamster Melanoma Cells In Vivo written by Georg Gerhard Schieferstein and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1974 with categories.
Culture Of Normal Human Blood Cells In A Diffusion Chamber System Ii Lymphocyte And Plasma Cell Kinetics
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Author :
language : en
Publisher:
Release Date : 1979
Culture Of Normal Human Blood Cells In A Diffusion Chamber System Ii Lymphocyte And Plasma Cell Kinetics written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1979 with categories.
Normal human blood leukocytes were cultured in Millipore diffusion chambers implanted into the peritoneal cavities of irradiated mice. The evaluation of survival and proliferation kinetics of cells in lymphyocytic series suggested that the lymphoid cells are formed from transition of small and/or large lymphocytes, and the lymphoblasts from the lymphoid cells. There was also evidence indicating that some of the cells in these two compartments are formed by proliferation. The evaluation of plasmacytic series suggested that the plasma cells are formed from plasmacytoid-lymphocytes by transition, and the latter from the transition of lymphocytes. In addition, relatively a small fraction of cells in these two compartments are formed by proliferation. mature plasma cells do not and immature plasma cells do proliferate. Estimation of magnitude of plasma cells formed in the cultures at day 18 indicated that at least one plasma cell is formed for every 6 normal human blood lymphocytes introduced into the culture.
Inhibition Of Granulopoiesis In Diffusion Chambers By A Granulocyte Chalone
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Author : T. J. MacVittie
language : en
Publisher:
Release Date : 1974
Inhibition Of Granulopoiesis In Diffusion Chambers By A Granulocyte Chalone written by T. J. MacVittie and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1974 with categories.
Granulocyte chalone obtained from media conditioned by rat granulocytes was tested on normal and vinblastine treated regenerating mouse marrow placed in an in vivo diffusion chamber culture system. Raw granulocyte chalone when injected several times during the early phase of culture significantly altered the cellular growth pattern as well as the parent-progeny relationships of both normal and regenerating marrow established within the chamber milieu. The total number of chamber cells (granulocytes plus macrophages) produced per inoculated stem cell was reduced by approximately 30 percent due to the action of granulocyte chalone. The reduction of granulocyte progeny may be the result of chalone acting during the early phase of culture to reduce the active growth fraction of granulocyte progenitor cells and thereby diminishing the amplification potential inherent in the initial cell inoculum.
Studies Of Ehrlich Ascites Tumor Cells
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Author : Elizabeth J. Barkins
language : en
Publisher:
Release Date : 1963
Studies Of Ehrlich Ascites Tumor Cells written by Elizabeth J. Barkins and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1963 with categories.
The Effect Of Whole Body Ultraviolet Exposure Of The Murine Host On Hela Cells In Diffusion Chamber Culture
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Author : William J. Fitzgerald
language : en
Publisher:
Release Date : 1983
The Effect Of Whole Body Ultraviolet Exposure Of The Murine Host On Hela Cells In Diffusion Chamber Culture written by William J. Fitzgerald and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983 with HeLa cells categories.