Effects Of Three Dimensional Structure Of Tissue Scaffolds On Animal Cell Culture
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Effects Of Three Dimensional Structure Of Tissue Scaffolds On Animal Cell Culture
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Author : Shubhayu Basu
language : en
Publisher:
Release Date : 2004
Effects Of Three Dimensional Structure Of Tissue Scaffolds On Animal Cell Culture written by Shubhayu Basu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Cell culture categories.
Abstract: Surface chemistry of the scaffold material, the fiber diameter, pore size, structure and configuration and porosity can regulate tissue development. The overall objective was to study the effect of the three dimensional structure of tissue scaffolds on animal cell culture. To achieve this, the growth of human astrocyte cells, secreting Glial-cell-line Derived Neurotrophic Factor (GDNF), in non-woven polyethylene terephthalate (PET) matrices was specifically targeted. The ability of GDNF to selectively nourish and regenerate dopaminergic neurons makes it an ideal candidate for the treatment of Parkinson's disease. On the basis of cell morphology, density and GDNF secretion, compressed PET matrices (porosity 88.8%, mean pore diameter 64 microns), treated with boiling sodium hydroxide, were found to be very effective in supporting high density growth of astrocytes. Most scaffolds used thus far, are anisotropic in their architecture and have a wide pore size distribution, making the task of identifying optimum values of the parameters important for tissue development even more difficult. However, microfabricated poly(lactic co-glycolic acid) (PLGA) scaffolds with a uniform structure supported a higher cell density, longer cell proliferation times than in non-uniform fibrous matrices, and induced less apoptosis, implying that uniformity in scaffold microstructure was a more important parameter than the mean pore size of the scaffolds. Cytocompatibility studies on PLGA scaffolds also revealed that cells could span in three-dimensional space over distances greater than four times their average length. To develop a fast convenient cell based model to study cell culture in three dimensional (3D) scaffolds, cells were transfected with an Enhanced Green Fluorescent Protein (EGFP) based reporter system. This allowed fast, sensitive and noninvasive monitoring of cell growth in suspension and could be used to detect the spatial distribution of these cells in 3D scaffolds. The fluorescence of these cells was linked to the S-phase of the cell cycle and decreased in a dosage dependent manner when the cells were treated with chemotherapeutic drugs 5-fluorouracil and doxorubicin, making these cells an ideal and reliable model for reporting cellular proliferation and drug effects on cell growth.
3d Stem Cell Culture
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Author : Joni H. Ylostalo
language : en
Publisher: MDPI
Release Date : 2021-01-07
3d Stem Cell Culture written by Joni H. Ylostalo and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-01-07 with Science categories.
Recently, stem cells have been drawing increasing interest in basic and translational research that aims to understand stem cell biology and generate new therapies for various disorders. Many stem cells can be cultured in 2D relatively easily using tissue culture plastic. However, many of these cultures do not represent the natural conditions of stem cells in the body. In the body, microenvironments include numerous supporting cells and molecules. Therefore, researchers and clinicians have sought ideal stem cell preparations for basic research and clinical applications, which may be attainable through 3D culture of stem cells. The 3D cultures mimic the conditions of the natural environment of stem cells better, as cells in 3D cultures exhibit many unique and desirable characteristics that could be beneficial for therapeutic interventions. 3D stem cell cultures may employ supporting structures, such as various matrices or scaffolds, in addition to stem cells, to support complex structures. This book brings together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cell culture techniques and also to highlight stem cell preparations for possible novel therapeutic applications.
3d Cell Culture
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Author : John Haycock
language : en
Publisher: Humana Press
Release Date : 2010-11-02
3d Cell Culture written by John Haycock and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-02 with Science categories.
Developed for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Protocols, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose. Beginning with an overview of the biological and materials scaffold requirements for successfully creating 3D models, the book delves into topics such as general scaffold design and fabrication techniques, models for bone, skin, cartilage, nerve, bladder, and hair follicles, and chapters on bioreactor design, imaging, and stem cells. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text but also require techniques described using a standard, convenient format.
Technology Platforms For 3d Cell Culture
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Author : Stefan Przyborski
language : en
Publisher: John Wiley & Sons
Release Date : 2017-03-03
Technology Platforms For 3d Cell Culture written by Stefan Przyborski and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-03-03 with Science categories.
Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs. Offers a comprehensive, focused guide to the current state-of-the-art technologies available for 3D cell culture Features contributions from leading developers and researchers active in 3D cell technology Gives clear instruction and guidance on performing specific 3D culture methods, along with colour illustrations and examples of where such technologies have been successfully applied Includes information on resources and technical support to help initiate the use of 3D culture methods
Effects Of Flow Patterns On Tissue Development In 3d Culture
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Author : Russell Ray Nakasone
language : en
Publisher:
Release Date : 2023
Effects Of Flow Patterns On Tissue Development In 3d Culture written by Russell Ray Nakasone and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023 with categories.
Objective: Three dimensional cell and tissue culture outcomes depend on mechanical forcesin their environment and sufficient delivery of nutrients and removal of waste. Flow and shear influence parenchymal cell distribution and vascular morphogenesis. Herein a bioreactor which can control fluid flow and shear patterns over large areas was developed as a tool for tissue engineering. Methods: A 12-inlet perfusion bioreactor was constructed to be compatible with multiple types of tissue engineering scaffolds including biopolymer hydrogels and macroporous sponges and can support the growth and viability of thick tissue scaffolds. Scaffolds containing mammalian cells cultured under flow were compared to static conditions and other flow patterns. Results: Flow patterns in the scaffolds were obtained using MRI flow maps. Hydrogels and commercial sponges were assessed for 3D culture compatibility and Gel 2 (a specific ECM hydrogel mixture), Collagen, and Gelatin sponges were found to be both optimal for cell growth and compatible with perfusion flow. Culture experiments determined the feasibility of our bioreactor in supporting thick tissue growth and viability and various flow patterns were shown to alter cell distribution, growth, and viability. Conclusion: We demonstrated the ability of the bioreactor to implement macroscopic, tunable flow patterns in a biocompatible environment suitable for cell and tissue culture and found distinct differences in tissue development from different flow patterns. Significance: The bioreactor will enable future studies on different flow patterns and their effects on cell growth and viability for medically relevant tissues and could inform on culture conditions that better mimic what occurs in vivo during tissue development.
Human Embryonic Stem Cells
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Author : Arlene Chiu
language : en
Publisher: Springer Science & Business Media
Release Date : 2003-08
Human Embryonic Stem Cells written by Arlene Chiu and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-08 with Science categories.
A discussion of all the key issues in the use of human pluripotent stem cells for treating degenerative diseases or for replacing tissues lost from trauma. On the practical side, the topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials. Regulatory issues are addressed in discussions of the ethical debate surrounding the derivation of human embryonic stem cells and the current policies governing their use in the United States and abroad, including the rules and conditions regulating federal funding and questions of intellectual property.
Biomaterials For Artificial Organs
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Author : Michael Lysaght
language : en
Publisher: Elsevier
Release Date : 2010-12-20
Biomaterials For Artificial Organs written by Michael Lysaght and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-20 with Technology & Engineering categories.
The worldwide demand for organ transplants far exceeds available donor organs. Consequently some patients die whilst waiting for a transplant. Synthetic alternatives are therefore imperative to improve the quality of, and in some cases, save people’s lives. Advances in biomaterials have generated a range of materials and devices for use either outside the body or through implantation to replace or assist functions which may have been lost through disease or injury. Biomaterials for artificial organs reviews the latest developments in biomaterials and investigates how they can be used to improve the quality and efficiency of artificial organs.Part one discusses commodity biomaterials including membranes for oxygenators and plasmafilters, titanium and cobalt chromium alloys for hips and knees, polymeric joint-bearing surfaces for total joint replacements, biomaterials for pacemakers, defibrillators and neurostimulators and mechanical and bioprosthetic heart valves. Part two goes on to investigate advanced and next generation biomaterials including small intestinal submucosa and other decullarized matrix biomaterials for tissue repair, new ceramics and composites for joint replacement surgery, biomaterials for improving the blood and tissue compatibility of total artificial hearts (TAH) and ventricular assist devices (VAD), nanostructured biomaterials for artificial tissues and organs and matrices for tissue engineering and regenerative medicine.With its distinguished editors and international team of contributors Biomaterials for artificial organs is an invaluable resource to researchers, scientists and academics concerned with the advancement of artificial organs. Reviews the latest developments in biomaterials and investigates how they can be used to improve the quality and efficiency of artificial organs Discusses commodity biomaterials including membranes for oxygenators and cobalt chromium alloys for hips and knees and polymeric joint-bearing surfaces for total joint replacements Further biomaterials utilised in pacemakers, defibrillators, neurostimulators and mechanical and bioprosthetic heart valve are also explored
International Farm Animal Wildlife And Food Safety Law
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Author : Gabriela Steier
language : en
Publisher: Springer
Release Date : 2017-01-09
International Farm Animal Wildlife And Food Safety Law written by Gabriela Steier and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-01-09 with Law categories.
This volume is an inspiring and breakthrough piece of academic scholarship and the first of its kind featuring a comprehensive reader-friendly approach to teach the intricacies of the various aspects of international farm animal, wildlife conservation, food safety and environmental protection law. The selected focus areas are grouped in sections, such as agrobiodiversity, fishing and aquaculture, pollinators and pesticides, soil management, industrial animal production and transportation, and international food trade. Farm animal welfare, environmental protection, biodiversity conservation, and food safety are the core of the selected chapters. Every chapter provides real-world examples to make the complex field easy to understand. With its systematic approach, this book is devoted to anyone interested in the subject, becomes a valuable resource for professionals working in food regulation, and provides a solid foundation for courses and master’s programs in animal law, environmental policy, food and agriculture law, and regulation of these subjects around the world. Through its emphasis on sustainable food production, this work offers a cutting-edge selection of evolving topics at the heart of the pertinent discourse. As one of its highlights, this books also provides “Tools for Change,” a unique compilation and analysis of laws from the major farm animal product trading nations. With these tools, practitioners, advocates, policy makers and other state-holders are equipped with information to start work toward improving farm animal welfare, wildlife conservation, and food safety through the use of law and policy.
Frontiers In Tissue Engineering
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Author : C.W. Patrick
language : en
Publisher: Elsevier
Release Date : 1998-02-20
Frontiers In Tissue Engineering written by C.W. Patrick and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-02-20 with Technology & Engineering categories.
Frontiers in Tissue Engineering is a carefully edited compilation of state-of-the-art contributions from an international authorship of experts in the diverse subjects that make up tissue engineering. A broad representation of the medical, scientific, industrial and regulatory community is detailed in the book. The work is an authoritative and comprehensive reference source for scientists and clinicians working in this emerging field. The book is divided into three parts: fundamentals and methods of tissue engineering, tissue engineering applied to specialised tissues, and tissue engineering applied to organs. The text offers many novel approaches, including a detailed coverage of cell-tissue interactions at cellular and molecular levels; cell-tissue surface, biochemical, and mechanical environments; biomaterials; engineering design; tissue-organ function; new approaches to tissue-organ regeneration and replacement of function; ethical considerations of tissue engineering; and government regulation of tissue-engineered products.
Scaffolding In Tissue Engineering
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Author : Peter X. Ma
language : en
Publisher: CRC Press
Release Date : 2005-08-19
Scaffolding In Tissue Engineering written by Peter X. Ma and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-08-19 with Medical categories.
The growing interest in scaffolding design and increasing research programs dedicated to regenerative medicine corroborate the need for Scaffolding in Tissue Engineering. While certain books and journal articles address various aspects in the field, this is the first current, comprehensive text focusing on scaffolding for tissue engineering.