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Electron Probe Microanalysis In Biology


Electron Probe Microanalysis In Biology
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Electron Probe Microanalysis


Electron Probe Microanalysis
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Author : Karl Zierold
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-08

Electron Probe Microanalysis written by Karl Zierold and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-08 with Science categories.


The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti cular diffusible ions (elements). 2. Electron probe microanaly sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.



Electron Probe Microanalysis In Biology


Electron Probe Microanalysis In Biology
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Author : David A. Erasmus
language : en
Publisher:
Release Date : 1978

Electron Probe Microanalysis In Biology written by David A. Erasmus and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1978 with Science categories.




Microbeam Analysis In Biology


Microbeam Analysis In Biology
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Author : Claude Lechene
language : en
Publisher: Elsevier
Release Date : 2012-12-02

Microbeam Analysis In Biology written by Claude Lechene and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Nature categories.


Microbeam Analysis in Biology contains the proceedings of a workshop on Biological X-Ray Microanalysis by Electron Beam Excitation, held in Boston, Massachusetts on August 25-26, 1977. This book focuses on the principles, techniques, and biological use of electron probe microanalysis, energy-loss spectroscopy, and ion probe microanalysis. This text reflects the emphasis of the workshop on presenting the principles of analysis, the optimization of operating conditions, the description of successful techniques for sample preparation and quantitation, the illustration of problems and pitfalls, and the direction of microbeam analysis in biology.



X Ray Microanalysis In Biology


X Ray Microanalysis In Biology
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Author : M. A. Hayat
language : en
Publisher:
Release Date : 1980

X Ray Microanalysis In Biology written by M. A. Hayat and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1980 with Science categories.




Microprobe Analysis In Biology And Medicine


Microprobe Analysis In Biology And Medicine
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Author : Patrick Echlin
language : en
Publisher:
Release Date : 1978

Microprobe Analysis In Biology And Medicine written by Patrick Echlin and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1978 with Biology categories.




X Ray Microanalysis In Biology


X Ray Microanalysis In Biology
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Author : David C. Sigee
language : en
Publisher: Cambridge University Press
Release Date : 1993-04-22

X Ray Microanalysis In Biology written by David C. Sigee and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-04-22 with Medical categories.


This book describes an integrated approach to the use of X-ray microanalysis in biology.



X Ray Microanalysis In Electron Microscopy For Biologists


X Ray Microanalysis In Electron Microscopy For Biologists
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Author : A. John Morgan
language : en
Publisher: Oxford University Press, USA
Release Date : 1985

X Ray Microanalysis In Electron Microscopy For Biologists written by A. John Morgan and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985 with Biology categories.


This compact guide provides a straightforward introduction to electron microprobe x-ray analysis, a nondestructive technique that greatly facilitates the study of the chemistry of cells. Assuming no prior knowledge of electron optics, Morgan explains the principle of x-ray production and detection, describes the various methods for converting measured x-ray intensities to element concentrations in thin specimens, and directs the reader to primary sources for more definitive practical guidelines. A painless introduction to a powerful laboratory technique, this book will be a useful aid for cell biologists, biological electron microscopists, and electrolyte physiologists.



Electron Probe Quantitation


Electron Probe Quantitation
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Author : K.F.J. Heinrich
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29

Electron Probe Quantitation written by K.F.J. Heinrich and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.


In 1968, the National Bureau of Standards (NBS) published Special Publication 298 "Quantitative Electron Probe Microanalysis," which contained proceedings of a seminar held on the subject at NBS in the summer of 1967. This publication received wide interest that continued through the years far beyond expectations. The present volume, also the result of a gathering of international experts, in 1988, at NBS (now the National Institute of Standards and Technology, NIST), is intended to fulfill the same purpose. After years of substantial agreement on the procedures of analysis and data evaluation, several sharply differentiated approaches have developed. These are described in this publi cation with all the details required for practical application. Neither the editors nor NIST wish to endorse any single approach. Rather, we hope that their exposition will stimulate the dialogue which is a prerequisite for technical progress. Additionally, it is expected that those active in research in electron probe microanalysis will appreciate more clearly the areas in which further investigations are warranted.



Micron High Resolution Microscopy And X Ray Microanalysis In Biology


Micron High Resolution Microscopy And X Ray Microanalysis In Biology
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Author :
language : en
Publisher:
Release Date : 1972

Micron High Resolution Microscopy And X Ray Microanalysis In Biology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1972 with categories.




Basic Methods In Biological X Ray Microanalysis


Basic Methods In Biological X Ray Microanalysis
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Author : Godfried M. Roomans
language : en
Publisher:
Release Date : 1983

Basic Methods In Biological X Ray Microanalysis written by Godfried M. Roomans and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983 with Science categories.