Elisa S Iquer
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Elisa
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Author : Robert S. Matson
language : en
Publisher: Springer Nature
Release Date : 2023-02-16
Elisa written by Robert S. Matson and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-02-16 with Medical categories.
This volume provides an understanding of how an immunoassay works, detailing the strengths, weaknesses, pitfalls. Chapters guide readers on how and when to appropriately utilize this powerful tool, examples of where the ELISA or similar immunoassay formats are currently being used, and newer techniques that may have a significant impact on future applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, ELISA: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.
Enzyme Linked Immunosorbent Assay Elisa
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Author : Samira Hosseini
language : en
Publisher: Springer
Release Date : 2017-12-30
Enzyme Linked Immunosorbent Assay Elisa written by Samira Hosseini and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-30 with Technology & Engineering categories.
This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.
The Elisa Guidebook
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Author : John R. Crowther
language : en
Publisher: Humana Press
Release Date : 2008-11-01
The Elisa Guidebook written by John R. Crowther and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-01 with Medical categories.
There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.
The Elisa Guidebook
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Author : John R. Crowther
language : en
Publisher: Humana Press
Release Date : 2000-08-17
The Elisa Guidebook written by John R. Crowther and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-08-17 with Science categories.
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
Elisa
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Author : John R. Crowther
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Elisa written by John R. Crowther and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
Elisa And Other Solid Phase Immunoassays
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Author : D. M. Kemeny
language : en
Publisher: John Wiley & Sons
Release Date : 1991-01-08
Elisa And Other Solid Phase Immunoassays written by D. M. Kemeny and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991-01-08 with Science categories.
This is a quick-reference manual on practical aspects of immunoassay. Providing a theoretical and practical basis for modern applications of solid-phase immunoassays, this text brings together experts who have used ELISA and other assays in a variety of fields. Contributors offer step-by-step guidance on how to use the various techniques involved in immunoassay. These techniques are extemely useful to laboratory-based researchers and technicians working on the detection of allergy, the AIDS virus, autoimmunity, etc. Chapters analyze the solid-phase supports used, the amplification systems, and the quantitation and affinity of antibodies and discuss the applications of assays to biology, immunology, and microbiology.
Elisa Mation
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Author : Jarrett N. Schmit
language : en
Publisher: CRC Press
Release Date : 1994-06-14
Elisa Mation written by Jarrett N. Schmit and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-06-14 with Science categories.
ELISA-Mation is a unique, color-animated, easy-to-operate introductory tutorial for the ELISA test widely used in medical and research laboratories. The manual uses both text and animated sequences to clearly describe the steps and reactions occurring in an ELISA test. ELISA-Mation also provides many educational uses including a review for professionals, an autotutorial for students, an adjunct to formal lectures, an in-class demonstration, and a training tool for laboratory personnel.
Elisa Theory And Practice
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Author : Crowther
language : en
Publisher:
Release Date : 2007-12-01
Elisa Theory And Practice written by Crowther and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-01 with categories.
The Immunoassay Handbook
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Author : David Wild
language : en
Publisher: Newnes
Release Date : 2013-01-21
The Immunoassay Handbook written by David Wild and has been published by Newnes this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-01-21 with Science categories.
The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. Provides a unique mix of theory, practical advice and applications, with numerous examples Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee Provides valuable chapter updates, now available on www.immunoassayhandbook.com
The Elisa Guidebook
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Author : John R. Crowther
language : en
Publisher:
Release Date : 2007-12-01
The Elisa Guidebook written by John R. Crowther and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-01 with Guidebook categories.
This text presents the fundamental aspects of the heterogeneous enzyme linked immunosorbent assay (ELISA). Designed for the newcomer as well as the experienced practitioner, it gives numerous examples of the use of ELISA.