The Elisa Guidebook
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The Elisa Guidebook
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Author : J. R. Crowther
language : en
Publisher:
Release Date : 2001
The Elisa Guidebook written by J. R. Crowther and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Medical categories.
This text presents the fundamental aspects of the heterogeneous enzyme linked immunosorbent assay (ELISA). Designed for the newcomer as well as the experienced practitioner, it gives numerous examples of the use of ELISA.
The Elisa Guidebook
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Author : John R. Crowther
language : en
Publisher: Humana
Release Date : 2010-11-19
The Elisa Guidebook written by John R. Crowther and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-19 with Medical categories.
There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.
The Elisa Guidebook
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Author : John R. Crowther
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
The Elisa Guidebook written by John R. Crowther and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
The Elisa Guidebook
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Author : John R. Crowther
language : en
Publisher:
Release Date : 2007-12-01
The Elisa Guidebook written by John R. Crowther and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-01 with Guidebook categories.
This text presents the fundamental aspects of the heterogeneous enzyme linked immunosorbent assay (ELISA). Designed for the newcomer as well as the experienced practitioner, it gives numerous examples of the use of ELISA.
Molecular Biomethods Handbook
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Author : John M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-11-04
Molecular Biomethods Handbook written by John M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-04 with Medical categories.
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR. In Part B, protein and cell-based methods are outlined, in subjects ranging from protein engineering to high throughput screening. Written by a well-established panel of research scientists, Molecular Biomethods Handbook, 2nd Edition provides an up to date collection of methods used regularly in the authors’ own research programs. This book will prove to be an invaluable reference for those engaged in or entering the field of molecular biology, and will provide the necessary background for those interested in setting up and using the latest molecular techniques.
Methods In Molecular Biology The Elisa Guidebook
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Author : John M. Walker
language : en
Publisher:
Release Date : 1984
Methods In Molecular Biology The Elisa Guidebook written by John M. Walker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols categories.
A Handbook On Techniques Of Molecular Biology
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Author : Dr. Manti Debnath
language : en
Publisher: OrangeBooks Publication
Release Date : 2024-11-29
A Handbook On Techniques Of Molecular Biology written by Dr. Manti Debnath and has been published by OrangeBooks Publication this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-11-29 with Science categories.
This handbook is a comprehensive guide for molecular biologists and researchers, offering detailed protocols and methodologies in molecular genetics and genomics. It covers key techniques such as DNA and RNA extraction, PCR, cDNA synthesis, and expression cloning. Advanced analytical tools like electrophoresis, blotting, and ELISA are discussed, along with modern technologies such as microarray, next-generation sequencing (NGS), and transcriptomics. The book also delves into genetic markers and polymorphic analyses using SSRs, RAPD, RFLP, AFLP, and innovative approaches like SAMPL. Designed as a practical resource, it is invaluable for gene expression studies, protein detection, and genetic analysis.
Elisa Guidebook The Methods In Molecular Biology
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Author :
language : en
Publisher:
Release Date : 2000
Elisa Guidebook The Methods In Molecular Biology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with categories.
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
Clinical Virology Manual
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Author : Steven C. Specter
language : en
Publisher: American Society for Microbiology Press
Release Date : 2009-05-09
Clinical Virology Manual written by Steven C. Specter and has been published by American Society for Microbiology Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-05-09 with Medical categories.
A comprehensive and updated volume for the clinical virologist. • Details laboratory procedures for detecting and handling viruses, from specimen requirements and quality assurance to virus detection and identification, from the fundamentals through the latest molecular methods. • Presents the most current knowledge on the wide range of specific viral pathogens. • Includes information on services provided by federal and state public health virology laboratories. • Provides essential information for clinicians and laboratory virologists.
Biochemistry And Molecular Biology Compendium
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Author : Roger L. Lundblad
language : en
Publisher: CRC Press
Release Date : 2019-11-11
Biochemistry And Molecular Biology Compendium written by Roger L. Lundblad and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-11-11 with Medical categories.
This book is an accessible resource offering practical information not found in more database-oriented resources. The first chapter lists acronyms with definitions, and a glossary of terms and subjects used in biochemistry, molecular biology, biotechnology, proteomics, genomics, and systems biology. There follows chapters on chemicals employed in biochemistry and molecular biology, complete with properties and structure drawings. Researchers will find this book to be a valuable tool that will save them time, as well as provide essential links to the roots of their science. Key selling features: Contains an extensive list of commonly used acronyms with definitions Offers a highly readable glossary for systems and techniques Provides comprehensive information for the validation of biotechnology assays and manufacturing processes Includes a list of Log P values, water solubility, and molecular weight for selected chemicals Gives a detailed listing of protease inhibitors and cocktails, as well as a list of buffers