A Handbook On Techniques Of Molecular Biology
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Molecular Biology Techniques
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Author : Heather B. Miller
language : en
Publisher: Academic Press
Release Date : 2011-10-18
Molecular Biology Techniques written by Heather B. Miller and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-18 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Basic Techniques In Molecular Biology
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Author : Stefan Surzycki
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Basic Techniques In Molecular Biology written by Stefan Surzycki and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Techniques In Molecular Biology
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Author : J.M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Techniques In Molecular Biology written by J.M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.
Handbook Of Molecular And Cellular Methods In Biology And Medicine Second Edition
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Author : Leland J. Cseke
language : en
Publisher: CRC Press
Release Date : 2003-11-24
Handbook Of Molecular And Cellular Methods In Biology And Medicine Second Edition written by Leland J. Cseke and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-11-24 with Science categories.
Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.
Advances In Molecular Techniques
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Author : Rakesh S. Sengar
language : en
Publisher: CRC Press
Release Date : 2018-05-11
Advances In Molecular Techniques written by Rakesh S. Sengar and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-11 with Medical categories.
Molecular genetics aims to comprehend biological activity at the gene sub-level. Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists.This book serves as a guide that introduces classic molecular biology techniques and advances in molecular and genetic engineering.
Molecular Biology Techniques
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Author : Walt Ream
language : en
Publisher: Academic Press
Release Date : 1998-11-17
Molecular Biology Techniques written by Walt Ream and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-11-17 with Science categories.
This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. - Provides well-tested experimental protocols for each technique - Lists the reagents and preparation of each experiment separately - Contains a complete schedule of experiments and the preparation required - Includes study questions at the end of each chapter
Advanced Methods In Molecular Biology And Biotechnology
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Author : Khalid Z. Masoodi
language : en
Publisher: Academic Press
Release Date : 2020-10-28
Advanced Methods In Molecular Biology And Biotechnology written by Khalid Z. Masoodi and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-28 with Science categories.
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Principles And Techniques Of Biochemistry And Molecular Biology
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Author : Keith Wilson
language : en
Publisher: Cambridge University Press
Release Date : 2010-03-04
Principles And Techniques Of Biochemistry And Molecular Biology written by Keith Wilson and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-03-04 with Medical categories.
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Wilson And Walker S Principles And Techniques Of Biochemistry And Molecular Biology
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Author : Keith Wilson
language : en
Publisher: Cambridge University Press
Release Date : 2018-04-19
Wilson And Walker S Principles And Techniques Of Biochemistry And Molecular Biology written by Keith Wilson and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-19 with Medical categories.
A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.
Techniques In Molecular Medicine
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Author : Friedhelm Hildebrandt
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Techniques In Molecular Medicine written by Friedhelm Hildebrandt and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Medical categories.
Standard molecular biology techniques are used widely in biomedical re search and have had a major impact on the understanding of physiologic processes and disease mechanisms. They also play an increasing role in mo lecular genetic diagnosis and new therapeutic approaches. This laboratory manual is designed for researchers who need reliable pro tocols for their laboratorywork. It provides a step by step approach to stan dard methods of molecular biology. A wide range of topics is covered by protocols for immediate application in the laboratory. These include pur ification, isolation, labeling, hybridization, sequencing and enzymatic mod ification ofDNA or RNA, polymerase chain reaction, cloning, libraries, and protein techniques. The manual is based on experience with a practical course in molecular biologytechniques, which was taught bythe authors. Since most methods in molecular biology recapitulate in a test tube mechanisms that occur natu rally in the cell, the first three chapters describe and illustrate basic mechan isms of molecular biology. U nderstanding more about these basic mechan isms will enable the researcher to apply techniques more specifically, based on the knowledge of their potential and limits. W e hope that users find this manual a helpful guide on their road to exciting insights.