Handbook Of Molecular And Cellular Methods In Biology And Medicine Second Edition
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Handbook Of Molecular And Cellular Methods In Biology And Medicine Second Edition
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Author : Leland J. Cseke
language : en
Publisher: CRC Press
Release Date : 2003-11-24
Handbook Of Molecular And Cellular Methods In Biology And Medicine Second Edition written by Leland J. Cseke and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-11-24 with Science categories.
Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.
Handbook Of Molecular And Cellular Methods In Biology And Medicine
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Author :
language : en
Publisher:
Release Date : 2004
Handbook Of Molecular And Cellular Methods In Biology And Medicine written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Cytology categories.
Handbook Of Molecular And Cellular Methods In Biology And Medicine
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Author : Leland J. Cseke
language : en
Publisher: CRC Press
Release Date : 2016-04-19
Handbook Of Molecular And Cellular Methods In Biology And Medicine written by Leland J. Cseke and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-19 with Medical categories.
Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology
Handbook Of Molecular And Cellular Methods In Biology And Medicine Third Edition
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Author : Leland J. Cseke
language : en
Publisher: CRC Press
Release Date : 2011-12-12
Handbook Of Molecular And Cellular Methods In Biology And Medicine Third Edition written by Leland J. Cseke and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-12-12 with Science categories.
Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology. Now in its third edition, this volume provides researchers with an updated tool kit that incorporates conventional as well as modern approaches to tackle biological and medicinal research in the post-genomics era. Significantly revised to address these recent changes, the editors have evaluated, revised, and sometimes replaced protocols with more efficient, more reliable, or simpler ones. The book has also been reorganized with section headings focusing on different biological levels connected to one another, taking into account the central dogma of biology (DNA → RNA → protein → metabolites). The book first explores traditional approaches and then moves to the modern "omics" approaches, including genomics, proteomics, and metabolomics. It also discusses the manipulation of biological systems (including RNAi) and macromolecular analyses, focusing on the use of microscopy. In each chapter, various notes and cautionary considerations are presented for potentially hazardous reagents. Filled with diagrams, tables, and figures to clarify methods, most chapters also contain Troubleshooting Guides indicating problems, possible causes, and solutions that may be incurred in carrying out the procedures. Researchers and scientists who master the techniques in this book are putting themselves at the cutting edge of biological and medicinal research.
Handbook Of Molecular And Cellular Methods In Biology And Medicine
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Author : Donghern Kim
language : en
Publisher: CRC Press
Release Date : 1995-05-15
Handbook Of Molecular And Cellular Methods In Biology And Medicine written by Donghern Kim and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-05-15 with Science categories.
Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!
Oxford Handbook Of Medical Sciences
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Author : Robert Wilkins
language : en
Publisher: OUP Oxford
Release Date : 2011-09-22
Oxford Handbook Of Medical Sciences written by Robert Wilkins and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-22 with Medical categories.
Written by biomedical scientists and clinicians, with the purpose of disseminating the fundamental scientific principles that underpin medicine, this new edition of the Oxford Handbook of Medical Sciences provides a clear, easily digestible account of basic cell physiology and biochemistry. It also includes an investigation of the traditional pillars of medicine (anatomy, physiology, biochemistry, pathology and pharmacology) integrated in the context of each of the major systems relevant to the human body. Cross-referenced to the Oxford Handbook of Clinical Medicine, and thoroughly illustrated, it is the ideal introduction to the medical sciences for medical students and biomedical scientists, as well as a valuable refresher for junior doctors.
Atlas Of Cell Organelles Fluorescence
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Author : Elli Kohen
language : en
Publisher: CRC Press
Release Date : 2003-12-29
Atlas Of Cell Organelles Fluorescence written by Elli Kohen and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-12-29 with Medical categories.
Containing over 150 original photomicrographs accompanied by protocol information, Atlas of Cell Organelles Fluorescence delineates organelles structures, interaction, and organization into complexes. It provides a collection that shows living cells under physiopathological conditions and in the context of treatment with carcinogens, xenobi
Chemical Biology
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Author : Jonathan E. Hempel
language : en
Publisher: Humana
Release Date : 2016-09-24
Chemical Biology written by Jonathan E. Hempel and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-09-24 with Science categories.
This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential components of the chemical biology tool discovery process, the book is organizes into four parts that focus on platforms for molecular discovery in in vitro cellular systems, in vivo chemical genetic screening protocols, and methods used to discover functional protein targets. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and informative, Chemical Biology: Methods and Protocols seeks to improve the success rate of the chemical biology field through the dissemination of detailed and experiential knowledge.
Morphology Methods
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Author : Ricardo V. Lloyd
language : en
Publisher: Springer Science & Business Media
Release Date : 2001-06-15
Morphology Methods written by Ricardo V. Lloyd and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-06-15 with Medical categories.
The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.
Handbook Of Molecular And Cellular Methods In Biology And Medicine
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Author : Donghern Kim
language : en
Publisher: CRC Press
Release Date : 2004-11-23
Handbook Of Molecular And Cellular Methods In Biology And Medicine written by Donghern Kim and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-11-23 with Science categories.
Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!