Engineered Protein Scaffolds As Tools For Studying Intracellular Proteins
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Engineered Protein Scaffolds As Tools For Studying Intracellular Proteins
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Author : Sabriyeh Alibai
language : en
Publisher:
Release Date : 2018
Engineered Protein Scaffolds As Tools For Studying Intracellular Proteins written by Sabriyeh Alibai and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with categories.
Molecules that bind to targets with high specificity and affinity are used as therapeutic and diagnostic agents in medicine and research. Protein and non-protein ligands are used for introduction of mutations within the binding region to increase the binding affinity. Phage-display technology is used to screen multiple scaffolds against target proteins to select for high affinity binders. Scaffolds are displayed on phage particles and can be tested for binding to targets with standard binding assays. Various target proteins are encoded by essential genes to disrupt cellular proliferation and were identified as fitness genes in CRISPR screens. Altogether, utilizing the two technologies facilitates making and screening of binders to intracellular targets and accelerates the production of highly efficient synthetic binders. To develop and optimize the technique, the Sidhu lab generated 27 protein scaffold libraries that were tested against 25 target proteins and selected several variants for further analysis in mammalian cells.
Protein Engineering
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Author : Huimin Zhao
language : en
Publisher: John Wiley & Sons
Release Date : 2021-08-23
Protein Engineering written by Huimin Zhao and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-08-23 with Science categories.
A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.
Intracellular Delivery
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Author : Aleš Prokop
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-05-26
Intracellular Delivery written by Aleš Prokop and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-05-26 with Science categories.
This book features a special subsection of Nanomedicine, an application of nanotechnology to achieve breakthroughs in healthcare. It exploits the improved and often novel physical, chemical and biological properties of materials only existent at the nanometer scale. As a consequence of small scale, nanosystems in most cases are efficiently uptaken by cells and appear to act at the intracellular level. Nanotechnology has the potential to improve diagnosis, treatment and follow-up of diseases, and includes targeted drug delivery and regenerative medicine; it creates new tools and methods that impact significantly upon existing conservative practices. This volume is a collection of authoritative reviews. In the introductory section we define the field (intracellular delivery). Then, the fundamental routes of nanodelivery devices, cellular uptake, types of delivery devices, particularly in terms of localized cellular delivery, both for small drug molecules, macromolecular drugs and genes; at the academic and applied levels, are covered. The following section is dedicated to enhancing delivery via special targeting motifs followed by the introduction of different types of intracellular nanodelivery devices (e.g. a brief description of their chemistry) and ways of producing these different devices. Finally, we put special emphasis on particular disease states and on other biomedical applications, whilst diagnostic and sensing issues are also included. Intracellular delivery / therapy is a highly topical which will stir great interest. Intracellular delivery enables much more efficient drug delivery since the impact (on different organelles and sites) is intracellular as the drug is not supplied externally within the blood stream. There is great potential for targeted delivery with improved localized delivery and efficacy.
Phage Display
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Author : Carlos F. Barbas
language : en
Publisher: CSHL Press
Release Date : 2001
Phage Display written by Carlos F. Barbas and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Affinity chromatography categories.
Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.
The Science And Applications Of Synthetic And Systems Biology
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Author : Institute of Medicine
language : en
Publisher: National Academies Press
Release Date : 2011-12-30
The Science And Applications Of Synthetic And Systems Biology written by Institute of Medicine and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-12-30 with Science categories.
Many potential applications of synthetic and systems biology are relevant to the challenges associated with the detection, surveillance, and responses to emerging and re-emerging infectious diseases. On March 14 and 15, 2011, the Institute of Medicine's (IOM's) Forum on Microbial Threats convened a public workshop in Washington, DC, to explore the current state of the science of synthetic biology, including its dependency on systems biology; discussed the different approaches that scientists are taking to engineer, or reengineer, biological systems; and discussed how the tools and approaches of synthetic and systems biology were being applied to mitigate the risks associated with emerging infectious diseases. The Science and Applications of Synthetic and Systems Biology is organized into sections as a topic-by-topic distillation of the presentations and discussions that took place at the workshop. Its purpose is to present information from relevant experience, to delineate a range of pivotal issues and their respective challenges, and to offer differing perspectives on the topic as discussed and described by the workshop participants. This report also includes a collection of individually authored papers and commentary.
Bone Tissue Engineering
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Author : Jeffrey O. Hollinger
language : en
Publisher: CRC Press
Release Date : 2004-10-14
Bone Tissue Engineering written by Jeffrey O. Hollinger and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-10-14 with Medical categories.
Focusing on bone biology, Bone Tissue Engineering integrates basic sciences with tissue engineering. It includes contributions from world-renowned researchers and clinicians who discuss key topics such as different models and approaches to bone tissue engineering, as well as exciting clinical applications for patients. Divided into four sections, t
Structural Aspects Of Protein Synthesis 2nd Edition
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Author : Anders Liljas
language : en
Publisher: World Scientific
Release Date : 2013-06-06
Structural Aspects Of Protein Synthesis 2nd Edition written by Anders Liljas and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-06 with Science categories.
This highly illustrated book provides an up-to-date description of the structure and function of the translation system including ribosomes, tRNAs, translation factors, antibiotics and aminoacyl-tRNA synthetases.Research on translation is undergoing rapid changes and is receiving significant attention as evidenced by the Nobel Prize in Chemistry 2009. The structural research by crystallography and cryo-EM forms part of an interactive framework that involves biochemistry and molecular computation. The book provides a comprehensive overview of translation in light of the structural results. It is a valuable resource for scientists in this and related fields, as well as for students taking courses with a focus on translation.There is no other book in this field currently except the previous edition of this book. The authors have for a long time worked in the field of structure and function of the translation system.
Plant Cyclotides
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Author :
language : en
Publisher: Academic Press
Release Date : 2015-11-24
Plant Cyclotides written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-11-24 with Science categories.
Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 76th volume, the series features several reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. Publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences Contains commentary by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology, and ecology This volume features reviews of the fast moving field of plant cyclotides
Mimicking The Extracellular Matrix
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Author : Gregory A Hudalla
language : en
Publisher: Royal Society of Chemistry
Release Date : 2019-03-15
Mimicking The Extracellular Matrix written by Gregory A Hudalla and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-03-15 with Technology & Engineering categories.
The extracellular matrix (ECM) is the focus of much interest in biology and bioengineering. Increasing understanding of the influence of the ECM on cell behaviour has led to the exciting possibilities of tissue engineering. Aside from new therapeutic tools, understanding the ECM is of course fundamental to basic cell biology research. Mimicking the Extracellular Matrix approaches this topic from both basic science and practical engineering perspectives. Seven topics are approached each in a pair of chapters, one with a biological approach and its partner with a bioengineering approach. Topics include the mechanical properties of the ECM, which outlines current knowledge of the ECM physical structure and reviewing state-of-the-art strategies to mimic its native microenvironments. The organisational characteristics of the ECM form the focus of another pair of chapters, where the collagen triple helix is discussed, followed by a review of advances in artificial reproduction of well-ordered systems using self-assembling peptides, or peptide amphiphiles. The balanced approach of this text gives it a broad appeal to those interested in the ECM from a range of backgrounds and disciplines. Suitable for undergraduates, postgraduates, and academics, this text aims to unify the current knowledge of ECM biology and matrix-mimicking biomaterials.
Therapeutic Proteins
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Author : Roland Kontermann
language : en
Publisher: John Wiley & Sons
Release Date : 2012-01-31
Therapeutic Proteins written by Roland Kontermann and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-01-31 with Science categories.
For this ready reference, the internationally renowned authority in the field, Roland Kontermann, has assembled a team of outstanding contributors from industry and academia to convey the worldwide knowledge on modifying therapeutic proteins in order to optimize their pharmacological potential. The result is a comprehensive work covering all approaches and aspects of the topic in one handy volume, making this indispensable reading for companies and research institutions working on the development of biopharmaceuticals.