Enzyme
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Enzyme Assays
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Author : Robert Eisenthal
language : en
Publisher: Practical Approach
Release Date : 2002
Enzyme Assays written by Robert Eisenthal and has been published by Practical Approach this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Analysis categories.
Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used tomeasure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gelelectrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening;statistical analysis of enzyme kinetic data; and the determination of active site concentration.This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.
Enzymes
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Author : Malcolm Dixon
language : en
Publisher: Elsevier
Release Date : 2014-06-05
Enzymes written by Malcolm Dixon and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-05 with Science categories.
Enzymes, Second Edition provides information pertinent to the developments in the field of enzymology. This book presents the properties of enzymes as chemical catalysts or simply as chemical substances. Organized into 13 chapters, this edition begins with an overview of the range of action or specificity of enzymes. This text then discusses the special techniques employed in the isolation of enzymes and explores the considerable progress in the study of the properties and functions of enzymes. Other chapters consider the mechanism of enzyme catalysis by more direct methods, including the use of isotopes. This book discusses as well the mechanism of the biosynthesis of enzymes and the means by which their chemical structure is determined by the genetic material of the chromosomes. The final chapter deals with the essential aspects of the enzymatic system linking energy-producing processes with energy-utilizing processes. This book is a valuable resource for biochemists, physical chemists, and research workers.
Fundamentals Of Enzyme Kinetics
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Author : Athel Cornish-Bowden
language : en
Publisher: Elsevier
Release Date : 2014-05-20
Fundamentals Of Enzyme Kinetics written by Athel Cornish-Bowden and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-20 with Science categories.
Fundamentals of Enzyme Kinetics details the rate of reactions catalyzed by different enzymes and the effects of varying the conditions on them. The book includes the basic principles of chemical kinetics, especially the order of a reaction and its rate constraints. The text also gives an introduction to enzyme kinetics - the idea of an enzyme-substrate complex; the Michaelis-Menten equation; the steady state treatment; and the validity of its assumption. Practical considerations, the derivation of steady-state rate equations, inhibitors and activators, and two-substrate reactions are also explained. Problems after the end of each chapter have also been added, as well as their solutions at the end of the book, to test the readers' learning. The text is highly recommended for undergraduate students in biochemistry who wish to study about enzymes or focus completely on enzymology, as most of the mathematics used in this book, which have been explained in detail to remove most barriers of understanding, is elementary.
Enzymes
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Author : U Satyanarayana
language : en
Publisher: Elsevier Health Sciences
Release Date : 2014-11-07
Enzymes written by U Satyanarayana and has been published by Elsevier Health Sciences this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-11-07 with Medical categories.
Enzymes Enzymes
Enzyme Chemistry
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Author : Colin J. Suckling
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Enzyme Chemistry written by Colin J. Suckling and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
In the molecular sciences, enzyme chemistry occupies a special niche as one of the major contact points between chemical and biological disciplines. The special properties of enzymes as selective and efficient catalysts are so central to current challenges to chemists that the development of enzyme chemistry in the past thirty years has been a major stimulus to chemical research in general. On the one hand studies of the intrinsic properties of enzymes and, on the other hand, their applications to synthesis, drug design, and biosynthesis have had an immense impact. This book brings together in one volume essays describing several such fields with emphasis on the applications. It would be unnecessarily repetitious to outline the approach and contents of the book in a Preface; the first short chapter is more eloquent than a formal Preface can be. I shall therefore encourage you to begin with the Introduction in Chapter 1 and here I wish to extend my warm thanks to those who have contributed to the production of this book: the authors for their acceptance of the overall concept of the book and for the thoughtfulness of their writing; Dr Charles Suckling, FRS and Professor Hamish Wood for their constructive criticism of the whole book; and Dr John Buckingham and his colleagues at Chapman and Hall for their efficiency and enthusiasm in transforming the typescripts into the book that you now hold. Colin J. Suckling University of Strathclyde Contributors Donald H.
Practical Enzymology
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Author : Hans Bisswanger
language : en
Publisher: John Wiley & Sons
Release Date : 2019-10-21
Practical Enzymology written by Hans Bisswanger and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-21 with Science categories.
A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.
Enzyme Handbook 14
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Author : Dietmar Schomburg
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Enzyme Handbook 14 written by Dietmar Schomburg and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Today, as the large international genome sequence projects are gaining a great amount of public attention and huge sequence data bases are created it be comes more and more obvious that we are very limited in our ability to access functional data for the gene products -the proteins, in particular for enzymes. Those data are inherently very difficult to collect, interpret and standardize as they are highly distributed among journals from different fields and are often sub ject to experimental conditions. Nevertheless a systematic collection is essential for our interpretation of the genome information and more so for possible appli cations of that knowledge in the fields of medicine, agriculture, etc .. Recent pro gress on enzyme immobilization, enzyme production, enzyme inhibition, coen zyme regeneration and enzyme engineering has opened up fascinating new fields for the potential application of enzymes in a large range of different areas. It is the functional profile of an enzyme that enables a biologist of physician to analyze a metabolic pathway and its disturbance; it is the substrate specificity of an enzyme which tells an analytical biochemist how to design an assay; it is the stability, specificity and efficiency of an enzyme which determines its usefulness in the biotechnical transformation of a molecule. And the sum of all these data will have to be considered when the designer of artificial biocatalysts has to choose the optimum prototype to start with.
The Chemistry Of Enzyme Action
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Author :
language : en
Publisher: Elsevier
Release Date : 1983-12-01
The Chemistry Of Enzyme Action written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983-12-01 with Science categories.
The Chemistry of Enzyme Action
A Study Of Enzymes
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Author : Stephen A. Kuby
language : en
Publisher: CRC Press
Release Date : 1990-11-16
A Study Of Enzymes written by Stephen A. Kuby and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-11-16 with Medical categories.
This comprehensive monograph consists of two parts: Volume I, entitled Enzyme Catalysis, Kinetics, and Substrate Binding; and Volume II, entitled Mechanism of Enzyme Action. Volume I focuses on several aspects of enzyme catalytic behavior, their steady-state and transient-state kinetics, and the thermodynamic properties of substrate binding. Packed with figures, tables, schemes, and photographs, this volume contains over 1,000 references, including references regarding enzymology's fascinating history. This comprehensive book is of particular interest to enzymology students, teachers, and researchers. Volume II presents selected "cutting edge" examples of techniques and approaches being pursued in biochemistry. This up-to-date resource includes 11 chapters, which illustrate important theoretical and practical aspects of enzyme mechanisms. It also features selected examples in which today's most important techniques, ideas, and theories are used to elaborate on the intricate nature of enzyme action mechanisms. This particular volume provides important information for both the novice and the seasoned investigator.
Enzyme Technologies For Pharmaceutical And Biotechnological Applications
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Author : Herbert A Kirst
language : en
Publisher: CRC Press
Release Date : 2001-06-05
Enzyme Technologies For Pharmaceutical And Biotechnological Applications written by Herbert A Kirst and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-06-05 with Science categories.
A review of enzyme function in human and animal health, this book covers basic principles and applications in antibiotic biosynthesis, biocatalysis, and screening and assay optimization, as well as new and emerging technologies in the biotechnological and pharmaceutical industries. It offers real-world examples of timely laboratory, pharmaceutical and manufacturing processes, including large-scale applications of enzyme manipulations.