Practical Enzymology
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Practical Enzymology
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Author : Hans Bisswanger
language : en
Publisher: John Wiley & Sons
Release Date : 2013-06-21
Practical Enzymology written by Hans Bisswanger and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-21 with Science categories.
The second edition of the perfect companion for practical course work in enzymology. Now with an improved selection of enzymatic assays based on key metabolic reactions, this book provides the detailed background to the enzymatic reaction and the enzyme per se. Particular emphasis is placed on troubleshooting with the described methods, and, in addition, the new four-color layout throughout features detailed protocols highlighted for easy recognition. www.wiley-vch.de/home/enzymology: A companion website provides animations for all figures together with supplementary material, for deeper understanding of the partially abstract matter.
Practical Enzymology
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Author : Hans Bisswanger
language : en
Publisher: John Wiley & Sons
Release Date : 2019-10-21
Practical Enzymology written by Hans Bisswanger and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-21 with Science categories.
A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.
Enzymes
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Author : Robert A. Copeland
language : en
Publisher: John Wiley & Sons
Release Date : 2000-04-10
Enzymes written by Robert A. Copeland and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-04-10 with Science categories.
Fully updated and expanded-a solid foundation for understanding experimental enzymology. This practical, up-to-date survey is designed for a broad spectrum of biological and chemical scientists who are beginning to delve into modern enzymology. Enzymes, Second Edition explains the structural complexities of proteins and enzymes and the mechanisms by which enzymes perform their catalytic functions. The book provides illustrative examples from the contemporary literature to guide the reader through concepts and data analysis procedures. Clear, well-written descriptions simplify the complex mathematical treatment of enzyme kinetic data, and numerous citations at the end of each chapter enable the reader to access the primary literature and more in-depth treatments of specific topics. This Second Edition of Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis features refined and expanded coverage of many concepts, while retaining the introductory nature of the book. Important new features include: * A new chapter on protein-ligand binding equilibria * Expanded coverage of chemical mechanisms in enzyme catalysis and experimental measurements of enzyme activity * Updated and refined discussions of enzyme inhibitors and multiple substrate reactions * Coverage of current practical applications to the study of enzymology Supplemented with appendices providing contact information for suppliers of reagents and equipment for enzyme studies, as well as a survey of useful Internet sites and computer software for enzymatic data analysis, Enzymes, Second Edition is the ultimate practical guide for scientists and students in biochemical, pharmaceutical, biotechnical, medicinal, and agricultural/food-related research.
Enzymes Catalysis Kinetics And Mechanisms
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Author : N.S. Punekar
language : en
Publisher: Springer
Release Date : 2018-11-11
Enzymes Catalysis Kinetics And Mechanisms written by N.S. Punekar and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-11-11 with Science categories.
This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to plan enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.
Electrophoresis Of Enzymes
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Author : Gunter M. Rothe
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Electrophoresis Of Enzymes written by Gunter M. Rothe and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The electrophoresis of enzymes and isoenzymes is a well established technique in biochemical, clinical, environmental, microbiological, botanical and forensic laboratories and classical electrophoresis is presently undergoing a remarkable revival. This book compiles facts and methods on enzyme electrophoresis widely dispersed in hundreds of publications. The author summarizes them in clearly readable tables, in many carefully worked out electrophoresis and more than 140 staining protocols. The exhaustive practical experience of the author and the wealth of material summarized and reviewed makes this book a "must" for every enzyme laboratory. It will supply the practitioner with profound information on state-of-the-art enzyme electrophoresis.
Enzymes Catalysis Kinetics And Mechanisms
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Author : Narayan S. Punekar
language : en
Publisher: Springer Nature
Release Date : 2025-02-01
Enzymes Catalysis Kinetics And Mechanisms written by Narayan S. Punekar and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-02-01 with Science categories.
The second edition of the textbook Enzymes- Catalysis, Kinetics, and Mechanisms focuses on the two broad mechanistic facets of enzymology namely, the chemical and the kinetic. It endeavors to bring out the synergy between enzyme structures and mechanisms. Written with a self-study approach in mind, the emphasis is on how to begin experiments with an enzyme and subsequently analyze the data. The book is divided into six major sections– 1) Enzyme Catalysis – A Perspective, 2) Enzyme Kinetic Practice and Measurements, 3) Elucidation of Kinetic Mechanisms, 4) Chemical Mechanisms and Catalysis, 5) Exploiting Enzymes, and 6) An end piece on Frontiers in Enzymology. The individual concepts are treated as stand-alone short sections. In case the reader needs to use any one concept, it should be possible with minimal cross-referencing to the rest of the book. Further, the book presents specialized techniques and complex approaches that require involved experimentation in theory with suitable references to guide the reader. The book is proposed more as a textbook in a self-learning mode to students of modern biology, particularly those with limited exposure to quantitative aspects and organic chemistry.
Enzyme Technologies For Pharmaceutical And Biotechnological Applications
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Author : Herbert A Kirst
language : en
Publisher: CRC Press
Release Date : 2001-06-05
Enzyme Technologies For Pharmaceutical And Biotechnological Applications written by Herbert A Kirst and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-06-05 with Science categories.
A review of enzyme function in human and animal health, this book covers basic principles and applications in antibiotic biosynthesis, biocatalysis, and screening and assay optimization, as well as new and emerging technologies in the biotechnological and pharmaceutical industries. It offers real-world examples of timely laboratory, pharmaceutical and manufacturing processes, including large-scale applications of enzyme manipulations.
Current Catalog
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Author : National Library of Medicine (U.S.)
language : en
Publisher:
Release Date : 1979
Current Catalog written by National Library of Medicine (U.S.) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1979 with Medicine categories.
Includes subject section, name section, and 1968-1970, technical reports.
Understanding Enzymes
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Author : Allan Svendsen
language : en
Publisher: CRC Press
Release Date : 2016-04-27
Understanding Enzymes written by Allan Svendsen and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-27 with Mathematics categories.
Understanding Enzymes: Function, Design, Engineering, and Analysis focuses on the understanding of enzyme function and optimization gained in the past decade, past enzyme function analysis, enzyme engineering, and growing insights from the simulation work and nanotechnology measurement of enzymes in action in vitro or in silico. The book also prese
Enzymes
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Author : T Palmer
language : en
Publisher: Elsevier
Release Date : 2007-04-04
Enzymes written by T Palmer and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-04-04 with Technology & Engineering categories.
In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course. - Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subject - Discusses techniques such as membrane chromatography, aqueous phase partitioning and engineering recombinant proteins for purification - Includes a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy