Esterases Lipases And Phospholipases
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Esterases Lipases And Phospholipases
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Author : M.I. Mackness
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-21
Esterases Lipases And Phospholipases written by M.I. Mackness and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-21 with Medical categories.
The NATO-Advanced Research Workshop "Esterases, Lipases and Phospholipases: From Structure to Clinical Significance" was held at the University of Bordeaux II, France from 22nd- 24th September 1993 under the Directorship of Professor Michel Clerc of the University of Bordeaux II. The meeting was organised by Hugues Chap (INSERM U 326, Toulouse, France), Georges Ferard (University of Strasbourg, France), Wolfgang Junge (University of Kiel, Germany) and Michael Mackness (University of Manchester, UK). In recent years it has become increasingly apparent that hydrolytic enzymes of the esterase, lipase and phospholipase type play central roles in the pathophysiology of many human diseases. The purpose of this NATO-ARW was to bring together experts (both clinical and scientific) in all three interrelated fields to review the current basic and clinical position and discuss future developments particularly with respect to future research aimed at determining the basic biochemical lesion involving hydrolytic enzymes involved in human disease and the use of these enzymes in diagnosis. As well as formal lectures from established researchers, the meeting also involved a number of lively round-table discussions on future developments and presentations from younger research workers, all of which are recorded in this Proceedings and which contribute to the success of the meeting.
Lipases And Phospholipases
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Author : Georgina Sandoval
language : en
Publisher: Humana
Release Date : 2019-12-10
Lipases And Phospholipases written by Georgina Sandoval and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-12-10 with Science categories.
This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Editionserves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes.
Lipase And Phospholipase Protocols
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Author : Mark Doolittle
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Lipase And Phospholipase Protocols written by Mark Doolittle and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
The lipases and phospholipases represent a diverse group of enzymes that are expressed in animals, plants, fungi, and bacteria. Their ubiquitous distribution among all species is a testament to the essential roles played by these enzymes in lipid storage, mobilization and metabolism, membrane homeostasis and remodeling, endocrine and immune functions, and signal tra- duction. In humans, lipases and phospholipases are also thought to contribute to complex diseases, such as atherosclerosis, obesity, arthritis, and cancer, as well as to single gene defects, such as Wolman's disease and Type I hyperlipoproteinemia. Enzymatically, the lipases are unique, since they hydrolyze substrates that are either insoluble, or only partly soluble, in aq- ous solvents; thus, enzyme catalysis takes place at a lipid-water interface. The interface comprises at least two, and often more, discrete bulk and s- face phases, in which the enzyme, substrate, and products oflipolysis disperse among these phases based on their physical properties. Furthermore, the d- tribution of these components changes continuously as lipolysis proceeds. Thus, the lipases and phospholipases are fundamentally different from any other enzyme because of the physical complexity of the environment in which catalysis occurs.
Lipases And Phospholipases In Drug Development
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Author : Günter Müller
language : en
Publisher: John Wiley & Sons
Release Date : 2006-03-06
Lipases And Phospholipases In Drug Development written by Günter Müller and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-03-06 with Science categories.
Lipases and Phospholipases are key control elements in mammalian metabolism. They share many common features that set them apart from other metabolic enzyme classes, most importantly their association with biological membranes. Their potential as drug targets for the treatment of metabolic diseases is widely recognized, and the first lipase inhibitor drugs have been successfully introduced. Providing drug developers with a firm foundation for lipase-centered drug design, the editors of this volume have assembled experts from different scientific disciplines to create a comprehensive handbook for all pharmaceutical chemists, biochemists and physiologists working with lipases. The authors examine fundamental aspects of lipase function in vitro and in vivo, explaining how this knowledge may be used to develop lipase assays. They also treat the physiological roles of lipases in normal and disordered metabolism, as well as strategies to target lipases for the treatment of diabetes, obesity and related disorders. Additional topics include the application of phospholipases for liposome-based drug delivery and their use as diagnostic tools.
Lipolytic Enzymes
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Author : Hans Brockerhoff
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Lipolytic Enzymes written by Hans Brockerhoff and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Lipolytic Enzymes focuses on the biochemistry of lipolytic enzymes, particularly, pancreatic lipase and phospholipase 2 as well as their structure and catalytic mechanism. It explores the kinetics of lipolysis, the digestive lipases of nonmammalian animals, the assay and purification of cholesterol esterases and phospholipases, the method phospholipases use in hydrolyzing phospholipids, and the adaptive mechanism of lipolytic enzymes at the lipid-water interface. Organized into eight chapters, this book begins with an overview of the importance of lipolytic enzymes, including their medical, therapeutic, food, and other industrial applications. It then proceeds with a discussion on the classification of lipolytic enzymes according to the type of bond they hydrolyze and the substrates on which they act. The next chapters look at the substrates and supersubstrates of lipolytic enzymes, along with their maximal velocity and the Michaelis constant. Moreover, the book talks about the detection and assay of lipases, the molecular properties of pancreatic cholesterol esterases, the stimulating effect of bile salts on cholesterol esterases, the hydrolytic cleavage of carboxyl esterases, and the occurrence and distribution of phosphohydrolases. A chapter discussing the two groups of lipolytic enzymes (the first containing enzymes of broad substrate specificity and not requiring cofactors; the second containing metalloenzymes with very narrow substrate requirements) concludes this book. This book is a valuable resource for chemists, biochemists, and those working in the field of nutritional sciences.
Cloning And Characterization Of Two Lipases And A Lysophospholipase From Aspergillus Niger
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Author : Shu-Sen Zhu
language : en
Publisher:
Release Date : 2006
Cloning And Characterization Of Two Lipases And A Lysophospholipase From Aspergillus Niger written by Shu-Sen Zhu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.
We identified from a collection of partially sequenced Aspergillus niger cDNA clones four cDNAs that were predicted to encode secreted lipases or phospholipases B/lysophospholipases. The deduced amino acid sequence of two of the four genes, lipA and lipB, exhibited approximately 50% identity with each other and with a lipase from the thermophilic fungus Thermomyces lanuginosus. The other two cDNAs, plbA and plbB, share amino acid sequence 63% identity with each other and about 65% identity with Aspergillus fumigatus PlbAp. We functionally expressed the four genes in Pichia pastoris and A. niger, and purified and characterized the recombinant proteins. LipA showed optimal activity at 40°C and pH 4.5-5.0. LipA was stable at a pH range of 2.2-10.6 and up to 70°C. The pH optimum for LipB was pH 3.5-4.0, and it was stable between pH 3.0 and pH 9.6. The temperature optimum for LipB was 15°C and it retained 70% of its peak activity at 0°C. LipB has the lowest temperature activity profile when compared with reported cold-adapted enzymes. Substrate specificity determination with triacylglycerols and p -nitrophenyl esters showed that both lipases preferred esters with middle- and long-chain fatty acids. The pH optimum for the lysophospholipase activity encoded by PlbA was 3.0 and the activity was stable during incubation from pH 2 to 8.6. The temperature optimum of PlbA was 50°C and it retained at least 80% activity when incubated at 50°C for 3 h. PlbA exhibited a rather broad specificity towards lysophospholipids with maximal activity on lysophosphatidy1choline and lysophosphatidylserine and preferred the shorter C12 substrate relative to substrates with 14 to 18 carbons. PlbA did not exhibit phospholipase B, A1 or A2, lipase or general esterase activity and appeared to be lysophospholipid specific. PlbA lysophospholipase activity displayed cooperative kinetics with a Hill coefficient of 2.036. Recombinant PlbB could only be expressed at an extremely low level in both Pichia pastoris and Aspergillus niger. We were unable to purify and characterize this enzyme.
Lipases
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Author : Paul Woolley
language : en
Publisher:
Release Date : 1994
Lipases written by Paul Woolley and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.
This 1994 book provides a fascinating account of the fast-moving field of lipase research. The contributions, written by active research workers, summarise developments in the field and give access to recent literature. It covers both the lipases proper (triglyceride lipases and the phospholipases. It gives a comprehensive picture of the state of knowledge of these enzymes, with a strong bias towards the fields that are attracting the greatest attention: their detailed molecular structure, their mechanism of action, their position in the evolution of enzymes, and their application both in the laboratory and industry. The book will continue to be of interest to those working in universities, in research institutes and in companies specialising in biotechnology. The book will also be a useful reference book for postgraduate students entering this field of research.
Intestinal Lipid Metabolism
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Author : Charles M. Mansbach
language : en
Publisher: Springer Science & Business Media
Release Date : 2000-11-30
Intestinal Lipid Metabolism written by Charles M. Mansbach and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-11-30 with Medical categories.
This book was stimulated by the enthusiasm shown by attendees at the meetings in Saxon River, VT, sponsored by the Federation ofAmerican Societies for Experimental Biology (FASEB), on the subject of the intestinal processing of lipids. When these meetings were first started in 1990, the original organizers, two of whom are editors ofthis volume (CMM and PT), had two major goals. The first was to bring together a diverse group ofinvestiga tors who had the common goal of gaining a better understanding of how the intestine ab sorbs lipids. The second was to stimulate the interest of younger individuals whom we wished to recruit into what we believed was an exciting and fruitful area ofresearch. Since that time, the field has opened up considerably with new questions being asked and new an swers obtained, suggesting that our original goals for the meetings were being met. In the same spirit, it occurred to us that there has not been a recentbook that draws to gethermuch ofthe informationavailableconcerninghow the intestineprocesses lipids. This book is intended to reach investigators with an interest in this area and their pre- and post doctoral students. The chapters are written by individuals who have a long-term interest in the areas about which they write, and many have been speakers at the subsequent FASEB conferences that have followed on the first.
Hydrolases In Organic Synthesis
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Author : Uwe Theo Bornscheuer
language : en
Publisher: John Wiley & Sons
Release Date : 2006-05-12
Hydrolases In Organic Synthesis written by Uwe Theo Bornscheuer and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-05-12 with Science categories.
From reviews to the first edtion: "Bornscheuer and Kazlauskas have set out, and succeeded, in producing a definitive manual on hydrolytic enzymes (especially lipases, esterases, and proteases) for organic chemists. This is quite simply the best book of its type and can be unreservedly recommended to organic chemists who have an interest in using hydrolytic enzymes in synthesis." (Nicholas J. Turner, University of Edinburgh) "The book is an indispensable source of information on the use of hydrolases in organic synthesis. The subject matter is very well set out, and the chapters are clearly written and presented from a critical viewpoint. Bornscheuer and Kazlauskas have succeeded admirably in describing the capabilities and limitations of the use of hydrolytic enzymes and in critically evaluating them. No library should be without the book." (Fritz Theil, WITEGA Angewandte Werkstoff-Forschung GmbH, Berlin) The second edition of this extremely successful and well-proven book presents recent developments in the use of hydrolases for organic synthesis, reflecting in particular the enormous progress made in enzyme discovery and optimization with a new chapter on "Protein Sources and Optimization of Biocatalyst Performance". The renowned authors survey the stereoselective reactions of hydrolases, especially lipases, esterases and proteases, giving researchers an overview of what has worked in the past so that they can judge how to solve their own synthetic problems. In total, the book contains over one thousand chemical structures, rounded off by some 1,800 invaluable references.
Industrial Enzymes
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Author : Julio Polaina
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-05-16
Industrial Enzymes written by Julio Polaina and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-05-16 with Science categories.
Recent developments in genetic engineering and protein chemistry are bringing ever more powerful means of analysis to bear on the study of enzyme structure. This volume reviews the most important types of industrial enzymes. In a balanced manner it covers three interrelated aspects of paramount importance for enzyme performance: three-dimensional protein structure, physicochemical and catalytic properties, and the range of both classical and novel applications.