Expression Of Cloned Genes In Prokaryotic And Eukaryotic Cells
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Expression Of Cloned Genes In Prokaryotic And Eukaryotic Cells
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Author : Takis S. Papas
language : en
Publisher: Elsevier Publishing Company
Release Date : 1983
Expression Of Cloned Genes In Prokaryotic And Eukaryotic Cells written by Takis S. Papas and has been published by Elsevier Publishing Company this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983 with Science categories.
Gene Amplification And Analysis
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Author : Takis S. Papas
language : en
Publisher:
Release Date : 1983
Gene Amplification And Analysis written by Takis S. Papas and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983 with categories.
Gene Amplification And Analysis Volume 3 Expression Of Cloned Genes In Prokaryotic And Eukaryotic Cells
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Author : TS. Papas
language : en
Publisher:
Release Date : 1983
Gene Amplification And Analysis Volume 3 Expression Of Cloned Genes In Prokaryotic And Eukaryotic Cells written by TS. Papas and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983 with categories.
Gene Cloning
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Author : David M. Glover
language : en
Publisher: Springer
Release Date : 1984
Gene Cloning written by David M. Glover and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Medical categories.
The principles of cloning DNA; Recombination and mutagenesis of DNA in vitro; Bacteriophage vectors; Expression of cloned DNAs in E. coli plasmids; The physical characterization of cloned DNA segments and their counterparts within chromosomes; Gene cloning in fungi and plants; Expressao of cloned genes in animal cless.
Genetic Transformation In Prokaryotic And Eukaryotic Cells
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Author : Endang Semiarti
language : en
Publisher:
Release Date : 2020
Genetic Transformation In Prokaryotic And Eukaryotic Cells written by Endang Semiarti and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with Science categories.
Improving the quality and quantity of an organism and its products can be approached by molecular characters enhancement through the insertion of a gene of interest into cells of the desired organism. Genetic transformation of an organism involves isolation, identification, cloning a gene of interest into a vector, and transferring the gene to the target organism. This chapter reviews the process of genetic transformation into the organism,Äôs cell from bacterial (Escherichia coli), yeast, plant (Onion, Tobacco, and Orchids), and mammalian. The discussion will be focused on the introduction of DNA molecules into plant cells and protoplast mediated by polyethylene glycol (PEG), electroporation, and gene gun using particle bombardment. Further discussion on the transient protein expression system of plant-based on protoplast, onion cell, and tobacco will also be covered in this chapter as well. The systems have been proven as a powerful tool for determining subcellular protein localization, protein-protein interactions, identifying gene function, and regulation. Finally, it can be clearly seen, the differences and similarities in the mechanism of genetic transformation both in prokaryotic and eukaryotic systems.
Recombinant Gene Expression
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Author : Paulina Balbas
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Recombinant Gene Expression written by Paulina Balbas and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.
Concepts Of Biology
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Author : Samantha Fowler
language : en
Publisher:
Release Date : 2018-01-07
Concepts Of Biology written by Samantha Fowler and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-07 with Science categories.
Concepts of Biology is designed for the single-semester introduction to biology course for non-science majors, which for many students is their only college-level science course. As such, this course represents an important opportunity for students to develop the necessary knowledge, tools, and skills to make informed decisions as they continue with their lives. Rather than being mired down with facts and vocabulary, the typical non-science major student needs information presented in a way that is easy to read and understand. Even more importantly, the content should be meaningful. Students do much better when they understand why biology is relevant to their everyday lives. For these reasons, Concepts of Biology is grounded on an evolutionary basis and includes exciting features that highlight careers in the biological sciences and everyday applications of the concepts at hand.We also strive to show the interconnectedness of topics within this extremely broad discipline. In order to meet the needs of today's instructors and students, we maintain the overall organization and coverage found in most syllabi for this course. A strength of Concepts of Biology is that instructors can customize the book, adapting it to the approach that works best in their classroom. Concepts of Biology also includes an innovative art program that incorporates critical thinking and clicker questions to help students understand--and apply--key concepts.
Laboratory Methods In Enzymology Dna
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Author :
language : en
Publisher: Elsevier
Release Date : 2013-09-02
Laboratory Methods In Enzymology Dna written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-09-02 with Science categories.
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on DNA
Cloning
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Author : Stephen D. Fairbanks
language : en
Publisher: Nova Publishers
Release Date : 2004
Cloning written by Stephen D. Fairbanks and has been published by Nova Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Science categories.
The terms 'recombinant DNA technology', 'DNA cloning', 'molecular cloning' or 'gene cloning' all refer to the same process: the transfer of a DNA fragment of interest from one organism to a self-replicating genetic element such as a bacterial plasmid. The DNA of interest can then be propagated in a foreign host cell. This technology has been around since the 1970s, and it has become a common practice in molecular biology labs today. Reproductive cloning is a technology used to generate an animal that has the same nuclear DNA as another currently or previously existing animal. Dolly was created by reproductive cloning technology. In a process called 'somatic cell nuclear transfer' (SCNT), scientists transfer genetic material from the nucleus of a donor adult cell to an egg whose nucleus, and thus its genetic material, has been removed. The reconstructed egg containing the DNA from a donor cell must be treated with chemicals or electric current in order to stimulate cell division. Once the cloned embryo reaches a suitable stage, it is transferred to the uterus of a female host where it continues to develop until birth. Therapeutic cloning, also called "embryo cloning," is the production of human embryos for use in research. The goal of this process is not to create cloned human beings, but rather to harvest stem cells that can be used to study human development and to treat disease. Stem cells are important to biomedical researchers because they can be used to generate virtually any type of specialised cell in the human body. This new book presents an up-to-date Chronology of Cloning along with current and selected abstracts dealing with cloning as well as a guide to books on the topic. Access to the abstract and books sections is provided by title, subject and author indexes.
Eukaryotic Gene Expression
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Author : Ajit Kumar
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-09
Eukaryotic Gene Expression written by Ajit Kumar and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-09 with Science categories.
The recent surge of interest in recombinant DNA research is understandable considering that biologists from all disciplines, using recently developed mo lecular techniques, can now study with great precision the structure and regulation of specific genes. As a discipline, molecular biology is no longer a mere subspeciality of biology or biochemistry: it is the new biology. Current approaches to the outstanding problems in virtually all the traditional disci plines in biology are now being explored using the recombinant DNA tech nology. In this atmosphere of rapid progress, the role of information exchange and swift publication becomes quite crucial. Consequently, there has been an equally rapid proliferation of symposia volumes and review articles, apart from the explosion in popular science magazines and news media, which are always ready to simplify and sensationalize the implications of recent dis coveries, often before the scientific community has had the opportunity to fully scrutinize the developments. Since many of the recent findings in this field have practical implications, quite often the symposia in molecular biology are sponsored by private industry and are of specialized interest and in any case quite expensive for students to participate in. Given that George Wash ington University is a teaching institution, our aim in sponsoring these Annual Spring Symposia is to provide, at cost, a forum for students and experts to discuss the latest developments in selected areas of great significance in biology. Additionally, since the University is located in Washington, D. C.