Extractive Fermentation Lactic Acid And Acetone Butanol Production
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Extractive Fermentation Lactic Acid And Acetone Butanol Production
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Author : Steve Ronald Roffler
language : en
Publisher:
Release Date : 1986
Extractive Fermentation Lactic Acid And Acetone Butanol Production written by Steve Ronald Roffler and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986 with categories.
Simultaneous Saccharification And Extractive Fermentation Of Pretreated Hardwood For Acetone Butanol Production
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Author : Minish Mahendrabhai Shah
language : en
Publisher:
Release Date : 1992
Simultaneous Saccharification And Extractive Fermentation Of Pretreated Hardwood For Acetone Butanol Production written by Minish Mahendrabhai Shah and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Acetone categories.
Acetone Butanol Ethanol Fermentation And Pervaporation By Clostridium Acetobutylicum B18
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Author : Qinghuang Geng
language : en
Publisher:
Release Date : 1995
Acetone Butanol Ethanol Fermentation And Pervaporation By Clostridium Acetobutylicum B18 written by Qinghuang Geng and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.
Engineering Microbial Fermentations
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Author : Zachary Cardwell Baer
language : en
Publisher:
Release Date : 2014
Engineering Microbial Fermentations written by Zachary Cardwell Baer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.
The batch fermentation of simple sugars to ethanol has thus far been the most successful process to displace transportation fuel consumption worldwide. However, the physical properities of ethanol limit its application in most of the world beyond 10-15 vol% blends with conventional gasoline. Additionally, ethanol is too volatile to be blended at an appreciable level with jet and diesel fuels. Alternative technologies are necessary to produce compounds that can be blended in all forms of transportation fuel and to higher levels. The biological production of longer chain oxygenates or hydrocarbons has been purposed to address this challenge. However, the increased toxicity of these molecules has limited their usefulness to-date. Alternatively, chemocatalytic routes to produce these fuel molecules do not suffer from toxicity issues but the highly functional saccharide-based feedstocks result in undesirable by-product formation and yield losses. We propose that by effectively combining these biological and chemocatalytic approaches efficient production of all classes of transportation fuels can be achieved. Clostridium acetobutylicum is a well-studied industrial bacterium capable of fermenting a wide-variety of saccharides into a mixture of acetone, butanol, and ethanol (ABE). These mixed products contain functionalities that are amenable to C-C bond formation through transition-metal catalysis. Thus enabling the production of a suite of long-chain oxygenates from a variety of sugar substrates. This dissertation describes three major topics associated with the integration biological and chemocatalytic processes for fuel production: (1) the initial discovery and evaluation of such a combined process, (2) tailoring the fermentation products and catalyst to favor diesel production, and (3) engineering tunable ethanol and acetone co-production in Escherichia coli. Additionally, a thorough and reproducible protocol describing both biological and chemocatalytic procedures are provided. Acetone, a product of the acetone-butanol-ethanol (ABE) fermentation, harbors a nucleophilic [alpha]-carbon, which is amenable to C-C bond formation with the electrophilic alcohols (ethanol and n-butanol) produced in ABE fermentation. This functionality enables the formation of higher molecular weight hydrocarbons similar to those found in current gasoline, jet, and diesel fuels. Using a palladium-catalyzed alkylation reaction efficient conversion of ABE fermentation products into ketones, ranging from 2-pentanone to 6-undecanone was achieved. Tuning of the reaction conditions permits production of either predominately gasoline or jet and diesel precursors. Glyceryl tributyrate was identified as a suitable extractant for selective in situ removal of both acetone and alcohols. Enabling simple integration of ABE fermentation and water sensitive chemical catalysis, while reducing the energy demand of the overall process. Additionally, extractive fermentation with glyceryl tributyrate removed several lignocellulosic-derived pretreatment inhibitors completely restoring solvent production titers. Tailoring both the biological fermentation and chemocatalytic reaction conditions predictably increased the production of sustainable diesel blendstocks from lignocellulosic sugar. More specifically, engineering the metabolism of C. acetobutylicum to produce isopropanol-butanol-ethanol (IBE) as opposed to ABE by expression of the secondary alcohol dehydrogenase (sadh) from C. beijerinckii strain B593. Coupled with the optimization of a more water-resistant alkylation catalyst, hydrotalcite-supported copper(II) or palladium (0). Furthermore, in silco predictions of extraction efficiency using COSMO-RS were employed to identify oleyl alcohol as a superior extractant for in situ removal of IBE. To achieve highly controllable acetone and ethanol production both metabolic and fermentation engineering approaches were employed. Strains capable of producing acetone without carboxylic acid reassimilation through a newly described pathway were developed. Furthermore, a more oxidized sugar-acid (gluconic acid) was used to drive production of acetone through redox balancing of NADH, predicatively reducing the molar ethanol:acetone ratio. Increases in the molar ethanol:acetone ratio were also described by the co-expression of either the aldehyde/alcohol dehydrogenase (adhE) from E. coli MG1655 or pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) from Z. mobilis. Acetone titers reached wild-type C. acetobutylicum levels by controlling the fermentation sparge rate and pH in a bioreactor. Finally, catalytic strategies to upgrade the co-produced ethanol and acetone at both low and high molar ratios are described. By leveraging the advantages of both biological fermentation and chemocatalytic reactions of fermentation products with well-defined functionality we are able to efficiently convert a variety of saccharides into long-chain oxygenates. These long-chain oxygenates can be blended with all forms of transportation fuels. Additionally, tuning the microorganism's metabolism, fermentation conditions, catalysis reaction, or all of the above can optimize the production for a specific class of fuel. The integration of mixed-product fermentation with chemical catalysis is thus a novel and potentially enabling route for the economical conversion of biomass into liquid transportation fuels.
Lactic Acid Production
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Author : Jordi Planes
language : en
Publisher:
Release Date : 1998
Lactic Acid Production written by Jordi Planes and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Lactic acid categories.
Gas Stripping As An In Situ Product Removal Technique In The Acetone Butanol Ethanol Fermentation Of Whey Permeate By Clostridium Acetobutylicum P262
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Author : Michael Howeler
language : en
Publisher:
Release Date : 1995
Gas Stripping As An In Situ Product Removal Technique In The Acetone Butanol Ethanol Fermentation Of Whey Permeate By Clostridium Acetobutylicum P262 written by Michael Howeler and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.
Extractive Acetone Butanol Fermentation
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Author : Michael Sierks
language : en
Publisher:
Release Date : 1984
Extractive Acetone Butanol Fermentation written by Michael Sierks and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Fermentation categories.
Technoeconomic Evaluation Of The Extractive Fermentation Of Butanol As A Guide To Research In This Area Of Biotechnology
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Author :
language : en
Publisher:
Release Date : 1991
Technoeconomic Evaluation Of The Extractive Fermentation Of Butanol As A Guide To Research In This Area Of Biotechnology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with categories.
Fermentation
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Author : Source Wikipedia
language : en
Publisher: University-Press.org
Release Date : 2013-09
Fermentation written by Source Wikipedia and has been published by University-Press.org this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-09 with categories.
Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Pages: 40. Chapters: Acetone-butanol-ethanol fermentation, Bio-fermentation technology, Bioconversion, Butanediol fermentation, Fermentation (biochemistry), Fermentation (food), Fermentation crock, Fermentative hydrogen production, Industrial fermentation, Lactic acid fermentation, Liebig-Pasteur dispute, List of microorganisms used in food and beverage preparation, Mixed acid fermentation, Pasteur effect, Solid-state fermentation, Wild Fermentation (book), Zymomonas mobilis.
Integrated Process For Microbial Solvent Production From Whey Permeate Final Report
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Author :
language : en
Publisher:
Release Date : 1995
Integrated Process For Microbial Solvent Production From Whey Permeate Final Report written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.
Acetone and butanol were historically produced through fermentation of carbohydrate raw materials. Conventional feedstocks such as grain and molasses, and the energy required to recover products by distillation, are too costly for traditional batch fermentation to compete with petrochemical synthesis. The authors proposed to evaluate an acetone-butanol-ethanol fermentation of acid whey permeate, a cheap carbohydrate source, using up-to-date bioreactor technology continuous fermentation with cell recycle and in-situ butanol recovery by gas stripping. Clostridium acetobutylicum P262 was the strain chosen, as it assimilates both the lactose and lactic acid in acid whey. Single-stage continuous culture proved unsuitable for butanol production, since productivity is low and cultures degenerated quickly. Two-stage culture improved productivity by a factor of two over batch runs. All continuous cultures showed major oscillations in cell density, substrate concentration and products formed. Under these conditions, cell recycle did not affect productivity in two-stage culture. Gas stripping with fermentor off-gases recovered a clean condensate of butanol and acetone at 70--90% yield and with purification factors of 14 to 35. Stripping maintained solvent concentrations in the range of 2--4 g/l even at the peak of solventogenesis, eliminating product inhibition. Gas stripping produced a 50% improvement in substrate uptake and a 10--20% improvement in solvent productivity.