Flow Cytometry Principles For Clinical Laboratory Practice
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Flow Cytometry Principles For Clinical Laboratory Practice
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Author : Marilyn A. Owens
language : en
Publisher: Wiley-Liss
Release Date : 1994-12-20
Flow Cytometry Principles For Clinical Laboratory Practice written by Marilyn A. Owens and has been published by Wiley-Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-12-20 with Medical categories.
FLOW CYTOMETRY PRINCIPLES FOR CLINICAL LABORATORY PRACTICE Quality Assurance for Quantitative Immunophenotyping Marilyn A. Owens and Michael R. Loken Flow Cytometry Principles for Clinical Laboratory Practice is an invaluable benchtop reference and introduction for first-time users of flow cytometry in clinical diagnostic laboratories. This manual provides authoritative coverage of accepted methods, quality controls, and standards for the cytometric analysis of clinical samples with particular relevance to CD4+ lymphocytes. CD4+ lymphocyte determination by immunophenotyping is the standard method for evaluating the status of HIV infection, and the single most frequent application of flow cytometry in a diagnostic setting. Flow Cytometry Principles for Clinical Laboratory Practice will appeal to clinical laboratory technologists, medical practitioners, and cytometry lab directors in their role as instructors of new personnel. In addition, it is a valuable resource for clinical and biomedical researchers, establishing quantitative cytometric systems for studies in immunology and hematology.
Clinical Flow Cytometry
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Author : Olga Kagan Weinberg
language : en
Publisher:
Release Date : 2019-02
Clinical Flow Cytometry written by Olga Kagan Weinberg and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-02 with categories.
This book is a focused review of clinical flow cytometry, and is meant to be helpful in daily clinical practice for those just beginning to learn flow cytometry as well as those with years of experience. Covering the basic principles of flow cytometry, and then engaging in detailed reviews of the flow cytometric evaluation of B-cells, plasma cells, T-cells, and myeloid cells, it packs a wide variety of immunophenotypic data into one volume. Moreover, this book covers both normal and abnormal findings for each lineage, and highlights key pitfalls to avoid making diagnostic mistakes. Many of the most common neoplastic entities are reviewed, and signature findings are highlighted. Using the updated nomenclature for clinical hematologic malignancies provided by the revised 4th edition of the WHO classification system, the book is current in its approach and content. Whenever possible, detailed colored examples of flow cytometric plots are provided to help convey the important diagnostic findings. Most importantly, a review of current applications of flow cytometry in minimal residual disease is provided to assist in both the development and interpretation of these assays. Written by experts in the field, the result is a practical resource for use as an everyday clinical reference.
Flow Cytometry
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Author : Marion G. Macey
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-11-03
Flow Cytometry written by Marion G. Macey and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-03 with Medical categories.
Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.
Principles And Clinical Applications Of Flow Cytometry
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Author : Tsieh Sun
language : en
Publisher:
Release Date : 1999
Principles And Clinical Applications Of Flow Cytometry written by Tsieh Sun and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Flow cytometry categories.
Cellular Diagnostics
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Author : Ulrich Sack
language : en
Publisher: Karger Medical and Scientific Publishers
Release Date : 2009-01-01
Cellular Diagnostics written by Ulrich Sack and has been published by Karger Medical and Scientific Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-01 with Medical categories.
This book is the updated English version of the 2006 German bestseller Zellulare Diagnostik, a comprehensive presentation of flow cytometry and its applications. While some techniques of immunophenotyping by flow cytometry already are routine procedures in the laboratory, new methods for the functional characterization of cells, the analysis of rare cells, and the diagnosis of complex materials have only begun to win wide recognition. New approaches such as slide-based cytometry will lead to an increase in the use of cytometric techniques. Multiparameter approaches will further improve analysis. The book provides a comprehensive and detailed compilation of all aspects of flow cytometry in research and the clinic. For newcomers it offers a thorough introduction, for advanced users, specific protocols and interpretation assistance.
Flow Cytometry
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Author : Marion G. Macey
language : en
Publisher: Blackwell Science Incorporated
Release Date : 1994
Flow Cytometry written by Marion G. Macey and has been published by Blackwell Science Incorporated this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.
Flow cytometers were developed in the early 1960s and used initially by research immunologists to sort and analyse cell subpopulations. The system analyses cells as they move in a liquid stream, through a laser beam, over an electronic sensing area. The light-scattering properties of the individual cells allow the identification of different cell types within a mixed population. Recent advances in technology, including the use of monoclonal antibodies, fluorescent dyes and powerful but cheap computers, mean that flow cytometry is now a routine part of clinical practice for haematologists and immunologists. The identification of cell subsets, leukaemia and various lymphomas is now commonplace. Cell cycle analysis and the measurement of DNA aberrations is now clinically feasible. The greater sensitivity of the new generations of bench-top flow cytometers has enabled the identification of bacteria from various sources, including water, sewage and body fluids and this has resulted in a dramatic increase in the use of these machines in microbiology. The editor has spent several years establishing the appropriate techniques for evaluating the ex vivo expression of antigens on leukocytes and has assembled a distinguished team of international contributors who provide practical advice on the application, scope and limitations of flow cytometry. This book will be of use to research workers, to clinical laboratory scientists and to clinicians who require an introduction and authoritative reference to flow cytometry, with an emphasis on clinical application. The distinguished team of contributors includes Richard Camplejohn, Leon Terstappen, Scott Cram and Terry Hoy, who have been at the forefront of flow cytometry development.
Flow Cytometry
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Author : Alice Longobardi Givan
language : en
Publisher: John Wiley & Sons
Release Date : 2001-07-23
Flow Cytometry written by Alice Longobardi Givan and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-07-23 with Science categories.
Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.
Flow Cytometry
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Author : Alice Longobardi Givan
language : en
Publisher: John Wiley & Sons
Release Date : 2013-04-10
Flow Cytometry written by Alice Longobardi Givan and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-10 with Science categories.
Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.
Practical Flow Cytometry
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Author : Howard M. Shapiro
language : en
Publisher: John Wiley & Sons
Release Date : 2005-02-25
Practical Flow Cytometry written by Howard M. Shapiro and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-02-25 with Science categories.
From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.
Flow Cytometry In Hematopathology
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Author : Doyen T. Nguyen
language : en
Publisher: Springer Science & Business Media
Release Date : 2002-11-26
Flow Cytometry In Hematopathology written by Doyen T. Nguyen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-11-26 with Medical categories.
Flow cytometry immunophenotyping of hematopoietic disorders is a complex and demanding exercise that requires a good understanding of cell lineages, developmental pathways, and physiological changes, as well as broad experience in hematopathology. The process includes several interrelated stages, from the initial medical decision regarding which hematologic c- dition is appropriate for FCM assay, to the final step of diagnosis whereby the FCM data is correlated with other relevant clinical and laboratory information. The actual FCM testing involves three major steps: pre-analytical (specimen processing, antibody staining), analytical (acquiring data on the flow cytometer) and post-analytical (data analysis and interpretation). The literature, including the latest FCM textbooks, provides ample information on the te- nical principles of FCM such as instrumentation, reagents and laboratory methods, as well as quality control and quality assurance. Similarly, correlations of morphologic findings and p- notypic profiles have been well covered in many publications. In contrast, much less attention has been given to the other equally important aspects of FCM immunophenotyping, especially data analysis. The latter is a crucial step by which a phenotypic profile is established. To bridge this gap in the literature, the focus of this book is more on FCM data analysis than laboratory methods and technical details. For the reader to become familiar with our data analysis strategy, an overview of our approach to the pre-analytical and analytical steps is also presented, with an emphasis on the pre-analytical aspects, which have been rarely touched upon in the literature.