Flowering Locus C Flc And The Regulation Of Flowering In Arabidopsis
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Flowering Locus C Flc And The Regulation Of Flowering In Arabidopsis
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Author : Melissa J. Hills
language : en
Publisher:
Release Date : 2005
Flowering Locus C Flc And The Regulation Of Flowering In Arabidopsis written by Melissa J. Hills and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Arabidopsis thaliana categories.
Arabidopsis Vernalization Independence Genes Are Involved In Flowering Locus C Activation
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Author : Hua Zhang (Ph. D. in genetics)
language : en
Publisher:
Release Date : 2004
Arabidopsis Vernalization Independence Genes Are Involved In Flowering Locus C Activation written by Hua Zhang (Ph. D. in genetics) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Arabidopsis categories.
Vernalization And Flowering Time Celebrating 20 Years Of Flc
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Author : Richard Amasino
language : en
Publisher: Frontiers Media SA
Release Date : 2021-11-30
Vernalization And Flowering Time Celebrating 20 Years Of Flc written by Richard Amasino and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-11-30 with Science categories.
Roles Of Histone Modifications In Arabidopsis Development
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Author : Lin Xu
language : en
Publisher:
Release Date : 2008
Roles Of Histone Modifications In Arabidopsis Development written by Lin Xu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with categories.
In Arabidopsis, the developmental transition from vegetative to reproductive growth is controlled by several genetic pathways. The autonomous pathway and the vernalization pathway converge at the regulation of FLOWERING LOCUS C (FLC) expression. Both activation and repression of FLC expression are controlled by epigenetic mechanisms. The first part of my thesis focuses on the study of loss-of-function mutants of SDG8 and SDG26, which encode SET domain proteins showing high homologies. Loss-of-function of SDG8, mutants sdg8-1 and sdg8-2, causes early flowering which is correlated with decreased expression of FLC and its homologues. By Western blot and ChIP, I demonstrated that levels of H3K36 di- and tri-methylation were greatly reduced while the level of H3K36 mono-methylation was increased in the sdg8 mutants. My study thus establishes that H3K36 di- and tri- but not mono-methylation is associated with transcription activation. In contrast to sdg8-1 and sdg8-2, sdg26-1 and sdg26-2 mutants showed a late flowering phenotype and an increased level of FLC expression. Nevertheless, molecular mechanism involved in FLC activation in sdg26-1 and sdg26-2 is currently unknown. In yeast, histone H2B mono-ubiquitylation is reported to precede H3K4 and H3K36 methylation during transcription initiation and elongation. The second part of my thesis focuses on molecular and functional characterization of AtUBC1 and AtUBC2 which encode homologues of Rad6, an E2 enzyme responsible for H2B mono-ubiquitylation in yeast. Although single mutants Atubc1-1 and Atubc2-1 have a normal phenotype, the double mutant Atubc1-1Atubc2-1 shows an early flowering phenotype. Our studies show that as in sdg8 mutants, expression of FLC and its homologues is reduced in Atubc1-1Atubc2-1. By Western blot, we showed that H2B mono-ubiquitination is significantly reduced in Atubc1-1Atubc2-1. Together these results suggest that H2B mono-ubiquitination and H3K36 methylation form a molecular pathway to regulate the FLC transcription, and play a crucial role in flowering. The last part of my thesis focuses on characterization of two novel RING domain genes LOC1 and LOC1, potentially involved in histone ubiquitination. I show that the double mutant loc1-1loc2-1 has a drastic phenotype of ectopic meristem formation. Molecular analysis revealed that the suppression of the Class I KNOX genes, STM, KNAT1/BP, KNAT2 and KNAT6, is released in loc1-1loc2-1. Physical and genetic interactions were observed between LOC1/LOC2 and CLF or LHP1, suggesting a Polycomb silencing pathway involved in repression of KNOX genes to confine shoot meristem.
Recent Advances In Flowering Time Control
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Author : Christian Jung
language : en
Publisher: Frontiers Media SA
Release Date : 2017-03-10
Recent Advances In Flowering Time Control written by Christian Jung and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-03-10 with categories.
The onset of flowering is an important step during the lifetime of a flowering plant. During the past two decades, there has been enormous progress in our understanding of how internal and external (environmental) cues control the transition to reproductive growth in plants. Many flowering time regulators have been identified from the model plant Arabidopsis thaliana. Most of them are assembled in regulatory pathways, which converge to central integrators which trigger the transition of the vegetative into an inflorescence meristem. For crop cultivation, the time of flowering is of upmost importance, because it determines yield. Phenotypic variation for this trait is largely controlled by genes, which were often modified during domestication or crop improvement. Understanding the genetic basis of flowering time regulation offers new opportunities for selection in plant breeding and for genome editing and genetic modification of crop species.
The Isolation And Characterization Of Flc
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Author : Scott Daniel Michaels
language : en
Publisher:
Release Date : 1999
The Isolation And Characterization Of Flc written by Scott Daniel Michaels and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with categories.
Histone H1 In Arabidopsis Thaliana
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Author : Ashley Loray Jones
language : en
Publisher:
Release Date : 2014
Histone H1 In Arabidopsis Thaliana written by Ashley Loray Jones and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.
Histone H1, or linker histone, are unique histones that bind to the nucleosome to facilitate higher order chromatin structure. The linker histones, when compared to the core histones that make up the nucleosome, are poorly understood especially in plants. Linker histones are vital for plant development as well as for cell cycle regulation, sharing many qualities with animal linker histones. In this report, the first two parts introduce the current literature of H1, including result from non-plant systems, and the third section is a research proposal describing a research project to elucidate the roles of linker histones on the regulation of FLOWERING LOCUS C (FLC) in Arabidopsis thaliana.
The Cloning And Characterization Of Early Flowering 4
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Author : Mark R. Doyle
language : en
Publisher:
Release Date : 2003
The Cloning And Characterization Of Early Flowering 4 written by Mark R. Doyle and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with categories.
The Isolation And Characterization Of Several Genes Involved In The Repression Of Flowering In Arabidopsis Thaliana
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Author : Colleen Marion Bizzel
language : en
Publisher:
Release Date : 2004
The Isolation And Characterization Of Several Genes Involved In The Repression Of Flowering In Arabidopsis Thaliana written by Colleen Marion Bizzel and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with categories.
Exploring Natural Variation In Flowering Time In Arabidopsis Thaliana
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Author : Jonathan David Werner
language : en
Publisher:
Release Date : 2004
Exploring Natural Variation In Flowering Time In Arabidopsis Thaliana written by Jonathan David Werner and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with categories.