Forensic Dna Profiling Protocols Methods In Molecular Biology
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Forensic Dna Profiling Protocols
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Author : Patrick J. Lincoln
language : en
Publisher: Springer Science & Business Media
Release Date : 1998-01-22
Forensic Dna Profiling Protocols written by Patrick J. Lincoln and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-01-22 with Medical categories.
This state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The methods include the recovery of DNA from a large range of sample types, analysis of DNA as single and multi-locus VNTR probes, PCR amplification of STR and other loci, and mitochondrial sequencing. The expert scientists writing here -- many from laboratories around the world -- also discuss how to interpret the results in cases of unknown identity and disputed parentage.-- Covers all steps from extraction of human DNA through to analysis and interpretation-- Takes advantage of new methodologies such as capillary electrophoresis-- Clear step-by-step instructions ensure unfailing reproducibility.
Forensic Dna Typing Protocols
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Author : Angel Carracedo
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Forensic Dna Typing Protocols written by Angel Carracedo and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.
Forensic Dna Profiling Protocols Methods In Molecular Biology
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Author : Patrick J. Lincoln
language : en
Publisher:
Release Date : 1998
Forensic Dna Profiling Protocols Methods In Molecular Biology written by Patrick J. Lincoln and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with categories.
Leading forensic experts from around the world describe in detail their time-proven methods for identity testing through DNA analysis. Their state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The book covers PCR- based test systems the now widely used STR typing systems (complete with details of both manual and automated detection systems) repeat unit mapping (MVR) direct phase minisequencing capillary electrophoresis and direct blotting. Also fully discussed are the recovery of DNA from a wide range of sample types (blood semen skeletal remains and saliva) species testing sex determination and mitochondrial DNA testing. SMP Version: This state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The methods include the recovery of DNA from a large range of sample types analysis of DNA as single and multi-locus VNTR probes PCR amplification of STR and other loci and mitochondrial sequencing.; The expert scientists writing here-many from laboratories around the world-also discuss how to interpret the results in cases of unknown identity and disputed parentage.
Forensic Dna Analysis
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Author : Catherine Cupples Connon
language : en
Publisher: Springer Nature
Release Date : 2023-08-14
Forensic Dna Analysis written by Catherine Cupples Connon and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-08-14 with Science categories.
This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchers interested in learning more about forensic DNA analysis procedures.
Dna Electrophoresis Protocols For Forensic Genetics
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Author : Antonio Alonso
language : en
Publisher: Humana Press
Release Date : 2011-12-08
Dna Electrophoresis Protocols For Forensic Genetics written by Antonio Alonso and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-12-08 with Medical categories.
Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of PCR amplicons obtained from DNA genomic markers with different inheritance patterns. In DNA Electrophoresis Protocols for Forensic Genetics, expert researchers in the field detail many of the protocols and methods which are now commonly used to perform forensic DNA profiling. It includes protocols for profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and mtDNA hypervariable regions HV1 and HV2 . Protocols for molecular identification of non-human species and mRNA profiling for body fluid identification are also included. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Review Of Forensic Dna Profiling Protocols
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Author : RE. Wenk
language : en
Publisher:
Release Date : 1999
Review Of Forensic Dna Profiling Protocols written by RE. Wenk and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with DNA categories.
This hard covered volume is one of the series (#98) of Methods in Molecular Biology and is directed to forensic scientists who need to apply practical methods of DNA analysis for the purpose of identifying either the origin of evidence samples or individuals. Mainstream chemical, interpretive and statistical methods are included.
Dna Fingerprinting Advancements And Future Endeavors
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Author : Hirak Ranjan Dash
language : en
Publisher: Springer
Release Date : 2018-11-01
Dna Fingerprinting Advancements And Future Endeavors written by Hirak Ranjan Dash and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-11-01 with Medical categories.
This book describes the basics and various applications of DNA fingerprinting, including in actual case studies. The book is divided in four modules; Module 1: Basics of DNA Fingerprinting, Module 2: Applications of DNA Fingerprinting, Module 3: DNA Fingerprinting: Case Studies, and Module 4: Future of DNA Fingerprinting. Each module consists of 4 to 5 chapters, written by reputed researchers, academics and forensic scientists from around the globe. The respective chapters cover e.g. related fields, the tools and techniques used, various genotyping kits, real-world case studies, ancient DNA and wild life forensics, molecular diagnosis of human diseases, legal aspects, microbial forensics and the economics of the DNA fingerprinting technique. The book offers a practical guide for professionals, graduate and post-graduate students in the fields of Forensic Science, Medicine, Genetics, Anthropology, Microbiology, and Zoology. It also serves as a useful reference resource, summarizing major technological advances in the field of DNA fingerprinting, the problems faced in this field of science and possible new solutions to these problems. Presently, DNA fingerprinting is utilized in solving the majority of criminal cases; as such, the book is also helpful for investigating agencies, as it includes representative case studies.
Principles And Practices Of Dna Analysis A Laboratory Manual For Forensic Dna Typing
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Author : Hirak Ranjan Dash
language : en
Publisher: Humana
Release Date : 2021-02-21
Principles And Practices Of Dna Analysis A Laboratory Manual For Forensic Dna Typing written by Hirak Ranjan Dash and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-02-21 with Medical categories.
The book presents hands-on protocols for conventional and advanced forensic DNA fingerprinting experiments. It includes manual, semi-automatic, and advanced automatic techniques for DNA extraction from different biological samples. It also discusses various qualitative and quantitative approaches for the assessment of extracted forensic DNA. It contains protocols for the amplification of short tandem repeat markers (STRs) for the amplification-based target enrichment of the forensic samples. Further, it examines genotyping of the STR loci through capillary electrophoresis and includes real-world case studies where forensic DNA analysis has been used in the criminal and civil disputes. The book concludes by presenting technological developments in the field of DNA forensic analysis. Suitable for beginners, it is a key reference resource on a wide variety of DNA profiling techniques and applications.
Forensic Dna Applications
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Author : Dragan Primorac
language : en
Publisher: CRC Press
Release Date : 2014-01-29
Forensic Dna Applications written by Dragan Primorac and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-29 with Law categories.
Forensic DNA Applications: An Interdisciplinary Perspective was developed as an outgrowth of a conference held by the International Society of Applied Biological Sciences. The topic was human genome–based applications in forensic science, anthropology, and individualized medicine. Assembling the contributions of contributors from numerous regions around the world, this volume is designed as both a textbook for forensic molecular biology students and a reference for practitioners and those in the legal system. The book begins with the history and development of DNA typing and profiling for criminal and civil purposes. It discusses the statistical interpretation of results with case examples, mitochondrial DNA testing, Y single nucleotide polymorphisms (SNPs) and short tandem repeats (STRs), and X SNP and STR testing. It also explores low copy number DNA typing, mixtures, and quality assurance and control. The second section examines the collection and preservation of biological evidence under a variety of different circumstances and the identification of human remains—including in mass disaster settings. It discusses applications to bioterrorism investigations, animal DNA testing in criminal cases, pedigree questions and wildlife forensic problems, applications in forensic entomology, and forensic botany. The third section explores recent developments and new technologies, including the rigorous identification of tissue of origin, mtDNA profiling using immobilized probe strips, chips and next-generation sequencing, the use of SNPs to ascertain phenotypic characteristics, and the "molecular autopsy" that looks at aspects of toxicogenetics and pharmacogenetics. The book concludes with a discussion on law, ethics, and policy. It examines the use of DNA evidence in the criminal justice system in both the United States and Europe, ethical issues in forensic laboratory practices, familial searches, DNA databases, ancestry searches, physical phenotyping, and report writing. The contributors also examine DNA applications in immigration and human trafficking cases and international perspectives on DNA databases.
Forensic Dna Typing Protocols
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Author : Angel Carracedo
language : en
Publisher: Humana Press
Release Date : 2004-11-30
Forensic Dna Typing Protocols written by Angel Carracedo and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-11-30 with Medical categories.
A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.