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Gene Expression In Ripening Kiwifruit


Gene Expression In Ripening Kiwifruit
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Gene Expression In Ripening Kiwifruit


Gene Expression In Ripening Kiwifruit
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Author : Jonathan Corey Tse
language : en
Publisher:
Release Date : 1987

Gene Expression In Ripening Kiwifruit written by Jonathan Corey Tse and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Gene expression categories.




Molecular Characterisation Of Genes Expressed During Fruit Development In Kiwifruit


Molecular Characterisation Of Genes Expressed During Fruit Development In Kiwifruit
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Author : Susan E. Ledger
language : en
Publisher:
Release Date : 1994

Molecular Characterisation Of Genes Expressed During Fruit Development In Kiwifruit written by Susan E. Ledger and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Kiwifruit categories.




Genetic Control Of Carotenoid Accumulation In Kiwifruit


Genetic Control Of Carotenoid Accumulation In Kiwifruit
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Author : Haidee Sau Man Tang
language : en
Publisher:
Release Date : 2018

Genetic Control Of Carotenoid Accumulation In Kiwifruit written by Haidee Sau Man Tang and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Carotenoids categories.


Actinidia macrosperma is a kiwifruit species that accumulates 40-fold more carotenoids in the fruit compared with green (A. deliciosa) or gold (A. chinensis) kiwifruit cultivars, giving it bright orange colour. Recently, in a number of species, a protein called ‘ORange’ (OR) was shown to facilitate carotenoid accumulation through regulating chloroplast to chromoplast transition, as well as post-transcriptionally increasing the enzyme activity of the carotenoid biosynthesis rate-limiting enzyme, phytoene synthase (PSY). How carotenoids are accumulated in kiwifruit is poorly understood. Therefore, it is of interest to determine whether OR regulates plastid transition and increase PSY activity to accumulate carotenoids in kiwifruit. Two OR and two OR-like genes were identified from the sequenced A. chinensis genome and A. macrosperma homologues were analysed. A fruit ripening series in three temperature conditions, and transient expression of AmOR genes in tobacco leaves, were used to elucidate the effect of AmOR and AmOR-like gene expression on metabolite change and plastid transition and to observe if temperature affects their expression. These were measured using a combination of metabolite analytical instruments, qPCR and visualized with light and fluorescence microscopy. AmOR-like genes were more highly expressed than AmOR genes. In particular, AmORL1 expression correlated with chlorophyll and chloroplast degradation during fruit ripening. In tobacco, here was no indication that AmORL1 facilitates carotenoid accumulation and therefore, ‘true’ chromoplasts (carotenoid storing plastids) are not produced in transient experiments. It appears that PSY expression is a pre-requisite for carotenoid accumulation. This study provides support for the hypothesis that ORL1 is a key regulator of chloroplast transition and expression of this gene in conjunction with PSY can increase carotenoid content.



The Physiology Molecular Biology And Biochemistry In Ripening And Stored Fruit


The Physiology Molecular Biology And Biochemistry In Ripening And Stored Fruit
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Author : Shifeng Cao
language : en
Publisher: Frontiers Media SA
Release Date : 2023-10-25

The Physiology Molecular Biology And Biochemistry In Ripening And Stored Fruit written by Shifeng Cao and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-10-25 with Science categories.




The Transcriptional Regulation Of Actinidia Chinensis Hort16a Fruit Ripening


The Transcriptional Regulation Of Actinidia Chinensis Hort16a Fruit Ripening
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Author : Peter Andrew McAtee
language : en
Publisher:
Release Date : 2014

The Transcriptional Regulation Of Actinidia Chinensis Hort16a Fruit Ripening written by Peter Andrew McAtee and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with Kiwifruit categories.


Fruit are important sources of food that can contribute a large portion of the fibre, water and vitamins required for a healthy human diet. The process of fruit ripening is a critical phase in the development of fruit as it is this process that renders fruit attractive and palatable for consumption. A better understanding of the mechanisms that regulate fruit ripening is likely to enhance our ability to predict and/or control the ripening process in fruit. This thesis investigated the molecular control of fruit ripening in the non-model crop plant Actinidia chinensis cv. 'Hort16A' that produces climacteric fleshy fruit similar to tomato. This crop species has particular relevance to the New Zealand economy, being its largest horticultural industry. Results in this thesis show that, at the physiological level, the ripening associated attributes of pericarp colour change, sugar accumulation, and pericarp softening initiate at distinctly different points in Actinidia chinensis 'Hort16A' fruit. The progression of each of these ripening attributes also occurred in multiple phases and at different rates. Analysis of the expression of genes associated with the progression of each ripening attribute demonstrated that multiple genes are likely to be required for each attribute to occur. These genes displayed complex expression patterns during the ripening of this fruit indicating that complex regulatory networks are likely to be required to regulate ripening in this fruit. Developmental and tissue specific regulation was observed in some genes involved in the progression of ripening attributes. This demonstrates a further level of complexity of ripening that occurs in a spatial and temporal manner. Treatment of ethylene resulted in the enhanced progression of pericarp softening and sugar accumulation but not pericarp colour change. The expression of genes involved in the progression of each of these attributes however all showed significant changes in response to ethylene treatment. Interestingly ethylene biosynthesis was not observed during the on-vine ripening of Actinidia chinensis 'Hort16A' fruit but this fruit can respond to ethylene treatment. This observation suggests that Actinidia chinensis 'Hort16A' fruit may have evolved a mechanism that involves two ripening phases that target different vectors for seed dissemination. It is however also possible that this fruit may respond to ethylene in this manner to enhance the progression of ripening in unfavourable abiotic conditions, thus ensuring some seed dispersal is achieved each year. Two kiwifruit NOR-like (AcNOL1 and AcNOL3) genes were stably expressed in non-ripening mutant tomato fruit. The fruit of these transgenic lines subsequently displayed an increased progression of ripening compared to non-ripening mutant fruit. The progression of ripening in these fruit corresponded with complex changes to the expression of distinctly different genes at different points of ripening. This shows that AcNOL1 and AcNOL3 regulate different ripening related aspects when expressed in tomato fruit. Transient expression of AcNOL1 and AcNOL3 in kiwifruit resulted in increased biosynthesis of ethylene, a key positive regulator of ripening. This indicates that these genes both have roles in the progression of ripening in kiwifruit. AcNOL1 and AcNOL3 display different expression profiles at distinct stages of ripening suggesting that they are likely to differentially contribute to the regulation of ripening in kiwifruit.



Regulation Of Fruit Ripening And Senescence


Regulation Of Fruit Ripening And Senescence
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Author : Carlos R. Figueroa
language : en
Publisher: Frontiers Media SA
Release Date : 2021-09-27

Regulation Of Fruit Ripening And Senescence written by Carlos R. Figueroa and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-09-27 with Science categories.




The Molecular Biology And Biochemistry Of Fruit Ripening


The Molecular Biology And Biochemistry Of Fruit Ripening
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Author : Graham Seymour
language : en
Publisher: John Wiley & Sons
Release Date : 2013-03-18

The Molecular Biology And Biochemistry Of Fruit Ripening written by Graham Seymour and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-18 with Science categories.


A comprehensive and mechanistic perspective on fruit ripening, emphasizing commonalities and differences between fruit groups and ripening processes. Fruits are an essential part of the human diet and contain important phytochemicals that provide protection against heart disease and cancers. Fruit ripening is of importance for human health and for industry-based strategies to harness natural variation, or genetic modification, for crop improvement. This book covers recent advances in the field of plant genomics and how these discoveries can be exploited to understand evolutionary processes and the complex network of hormonal and genetic control of ripening. The book explains the physiochemical and molecular changes in fruit that impact its quality, and recent developments in understanding of the genetic, molecular and biochemical basis for colour, flavour and texture. It is a valuable resource for plant and crop researchers and professionals, agricultural engineers, horticulturists, and food scientists. Summary: Reviews the physiochemical and molecular changes in fruit which impact flavour, texture, and colour Covers recent advances in genomics on the genetic, molecular, and biochemical basis of fruit quality Integrates information on both hormonal and genetic control of ripening Relevant for basic researchers and applied scientists



Kiwifruit Ripening Genes


Kiwifruit Ripening Genes
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Author : Fiona May Henderson
language : en
Publisher:
Release Date : 1987

Kiwifruit Ripening Genes written by Fiona May Henderson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Kiwifruit categories.




Fruit Ripening


Fruit Ripening
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Author : Pravendra Nath
language : en
Publisher: CABI
Release Date : 2014-07-16

Fruit Ripening written by Pravendra Nath and has been published by CABI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-07-16 with Technology & Engineering categories.


Fruit ripening is an important aspect of fruit production. The timing of it affects supply chains and buying behaviour, and for consumers ripeness not only affects perceptions of health but has nutritional effects too. Ripeness is closely related to spoilage which has a major financial impact on agricultural industries. Currently there are fast moving developments in knowledge of the factors affecting fruit ripeness, and this up-to-date monograph seeks to draw together the disparate research in this area. The aim of the book is to produce a comprehensive account covering almost every area related to fruit ripening including the latest molecular mechanisms regulating fruit ripening, its impact on human nutrition and emerging research and technologies.



Core Darkening Of Zesh004 Green14 Kiwifruit


Core Darkening Of Zesh004 Green14 Kiwifruit
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Author : Hannah Elizabeth Lloyd
language : en
Publisher:
Release Date : 2016

Core Darkening Of Zesh004 Green14 Kiwifruit written by Hannah Elizabeth Lloyd and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with Kiwifruit categories.


‘Zesh004’, commonly known as ‘Green14’, is an early maturing green-fleshed, sweet-fruited, Actinidia chinensis x A. deliciosa hybrid that has been developed by Zespri Group Ltd in partnership with the New Zealand Institute for Plant & Food Research Ltd. A darkening of the core tissue has been observed in some fruit, usually after a period of cold storage. This may be unacceptable to consumers and needs to be avoided to obtain a high premium for this product. The aim of this study was to characterise this dark core appearance, and determine whether it is due to a chilling injury or is simply the tissue becoming senescent (over-ripe). A trial was designed to investigate the senescence vs chilling hypothesis. The trial included fruit harvested at different maturities (early, mid and late) which were ripened with and without cold storage. The incidence and severity of the dark core appearance was quantified by eye and by computational image analysis. Cell walls from core tissue were further examined using microscopy and gene expression analyses to investigate any biochemical differences between the darker disordered core and non-disordered core tissue. The dark core appearance was observed in overripe fruit that were not cold stored. However, a positive correlation was observed between the dark core appearance and length of cold storage. A negative correlation was observed between the dark core appearance and the maturity of fruit at harvest. These results suggest that dark core appearance has a chilling injury component but also a senescence-related component. The dark core appearance developed from the stylar end towards the stem end of the fruit as observed in the scanned images of cut fruit. The microscopy analysis suggested that the stylar end was more advanced in ripening than the stem end of the fruit. At the molecular level however, genes for cell wall degradation enzymes had higher expression in the stem end samples mainly compared with the stylar end, especially after a longer period of cold storage. This suggests the dark core appearance may be due to expression of cell wall genes becoming uncoordinated due to cold storage, or that in the stylar end with advanced dark core appearance and tissue going into senescence, gene expression has already started to cease. In conclusion, there is an element of both chilling and senescence involvement in the development of the dark core. Further research could determine how this occurs on a cellular and subcellular level. Key words Kiwifruit, core, cell wall, chilling injury, cold storage, senescence, ripening