Henipavirus
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Henipavirus
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Author : Benhur Lee
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-09-05
Henipavirus written by Benhur Lee and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-09-05 with Medical categories.
Henipaviruses form a new genus of emerging paramyxoviruses that are the deadliest human pathogens within the Paramyxoviridae family. This volume deals with the many facets of henipavirus biology, and covers our current understanding regarding the ecology, molecular virology, and pathogenesis of henipavirus infections. It is an international effort written by a multidisciplinary panel of experts at the front lines of research into this lethal emerging group of paramyxoviruses. The first section introduces the epidemiology and ecology of Nipah and Hendra viruses in their respective endemic areas, including a first-hand account of the discovery of Nipah virus during its initial outbreak in Malaysia; the next section documents the molecular virology of henipaviruses, and the substantial advances made towards understanding the unique features of henipavirus entry and tropism; and this is followed by accounts of the clinical and pathologic features of henipavirus infections in their human and naturally infected animal hosts. The next sections on pathogenesis provide a comprehensive reference on how henipaviruses counteract the innate immune system, and the relevant pathogenic features in animal challenge models developed to test potential therapeutic strategies. The final sections describe our current and future capabilities for diagnosis and control, including an account of potentially effective immunization strategies that are currently being tested. This book will not only serve as a useful reference for the henipavirus field; it will be useful to basic and animal virologists, ecologists, epidemiologists, physicians, and others interested in emerging infectious viral diseases, as it showcases the multidisciplinary efforts required to understand the genesis, spread and hopefully, control, of a group of lethal emerging zoonotic pathogens.
Henipavirus
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Author : Benhur Lee
language : en
Publisher: Springer
Release Date : 2012-09-06
Henipavirus written by Benhur Lee and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-09-06 with Medical categories.
Henipaviruses form a new genus of emerging paramyxoviruses that are the deadliest human pathogens within the Paramyxoviridae family. This volume deals with the many facets of henipavirus biology, and covers our current understanding regarding the ecology, molecular virology, and pathogenesis of henipavirus infections. It is an international effort written by a multidisciplinary panel of experts at the front lines of research into this lethal emerging group of paramyxoviruses. The first section introduces the epidemiology and ecology of Nipah and Hendra viruses in their respective endemic areas, including a first-hand account of the discovery of Nipah virus during its initial outbreak in Malaysia; the next section documents the molecular virology of henipaviruses, and the substantial advances made towards understanding the unique features of henipavirus entry and tropism; and this is followed by accounts of the clinical and pathologic features of henipavirus infections in their human and naturally infected animal hosts. The next sections on pathogenesis provide a comprehensive reference on how henipaviruses counteract the innate immune system, and the relevant pathogenic features in animal challenge models developed to test potential therapeutic strategies. The final sections describe our current and future capabilities for diagnosis and control, including an account of potentially effective immunization strategies that are currently being tested. This book will not only serve as a useful reference for the henipavirus field; it will be useful to basic and animal virologists, ecologists, epidemiologists, physicians, and others interested in emerging infectious viral diseases, as it showcases the multidisciplinary efforts required to understand the genesis, spread and hopefully, control, of a group of lethal emerging zoonotic pathogens.
Henipavirus Advances In Research And Treatment 2011 Edition
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Author :
language : en
Publisher: ScholarlyEditions
Release Date : 2012-01-09
Henipavirus Advances In Research And Treatment 2011 Edition written by and has been published by ScholarlyEditions this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-01-09 with Medical categories.
Henipavirus: Advances in Research and Treatment: 2011 Edition is a ScholarlyPaper™ that delivers timely, authoritative, and intensively focused information about Henipavirus in a compact format. The editors have built Henipavirus: Advances in Research and Treatment: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Henipavirus in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Henipavirus: Advances in Research and Treatment: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Nipah Virus
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Author : Alexander N. Freiberg
language : en
Publisher: Springer Nature
Release Date : 2023-09-24
Nipah Virus written by Alexander N. Freiberg and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-09-24 with Medical categories.
This volume summarizes key methods and advancements in the field of henipavirus molecular biology. Chapters guide readers through protocols focusing on emerging bat-borne paramyxoviruses as well as pathogenicity caused by Nipah and Hendra viruses. Protocols are also included to help develop diagnostic tools, antiviral therapeutics, and vaccine creation. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Nipah Virus: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Henipavirus Advances In Research And Treatment 2012 Edition
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Author :
language : en
Publisher: ScholarlyEditions
Release Date : 2012-12-26
Henipavirus Advances In Research And Treatment 2012 Edition written by and has been published by ScholarlyEditions this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-26 with Science categories.
Henipavirus—Advances in Research and Treatment: 2012 Edition is a ScholarlyBrief™ that delivers timely, authoritative, comprehensive, and specialized information about Henipavirus in a concise format. The editors have built Henipavirus—Advances in Research and Treatment: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Henipavirus in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Henipavirus—Advances in Research and Treatment: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Elucidating The Role Of Host Factors In Henipavirus Budding
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Author : Fnu Sabahat Gazal
language : en
Publisher:
Release Date : 2021
Elucidating The Role Of Host Factors In Henipavirus Budding written by Fnu Sabahat Gazal and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021 with categories.
Nipah and Hendra viruses are highly virulent, zoonotic BSL-4 agents that emerged in 1990's and since then have been responsible for causing hundreds of human fatalities throughout Asia and Australia. Henipaviruses belong to family Paramyxoviridae and have been found to depend on ESCRT machinery to facilitate live virus budding. However, previous studies have shown that henipavirus M VLP budding is ESCRT independent. In this study, we showed that henipavirus F VLP budding is ESCRT dependent and ubiquitin dependent. We further showed the Nedd4 family ubiquitin ligase, Nedd4L enhances henipavirus F VLP budding and that ablation of ubiquitin ligase activity of Nedd4L inhibits F VLP budding. These findings provide first indication that in case of henipaviruses, Nedd4L is recruited by a viral transmembrane glycoprotein to facilitate budding in an ESCRT dependent manner. For some paramyxoviruses like PIV5 and mumps virus (that lack any of the conventional late domains), a host protein named angiomotin like 1 (AmotL1) has been found to play an important role in budding by acting as a bridge between the viral M protein and Nedd4 family ubiquitin ligases. Since henipaviruses also lack any of the well characterized late domains and as we found henipavirus F VLP budding to be ESCRT and ubiquitin dependent, the study further aimed to determine the role of angiomotins in henipavirus F VLP budding. Angiomotin family of proteins include angiomotin (Amot) and angiomotin like proteins 1 and 2 viz. AmotL1 and AmotL2. Using CRISPR/ Cas9 mediated knockout of Amot or AmotL1, we showed that knockout of angiomotins inhibit henipavirus F VLP budding but had no significant effect on M VLP budding. Double knockout of Amot and AmotL1 resulted in near complete inhibition of F VLP budding. Angiomotins have PPXY domains through which they interact with Nedd4 family ubiquitin ligases. Here we have shown that the PPXY domains of AmotL1 play an important role in Hendra virus F VLP budding as the overexpression of mutant AmotL1 lacking LPTY and PPXY sequences lead to inhibition of Hendra virus F VLP budding. Co-localization studies showed that GFP fused to cytoplasmic tail of Hendra virus F co-localizes with AmotL1 and also Nedd4L. We propose that henipavirus F proteins instead of directly harboring PPXY sequences, bind to PPXY containing angiomotins and thereby recruit Nedd4L and thus ESCRT in a way similar to that observed with viruses harboring a PPXY late domain.
Identification Of The Nipah Virus Receptors
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Author : Oscar Alfredo Negrete
language : en
Publisher:
Release Date : 2007
Identification Of The Nipah Virus Receptors written by Oscar Alfredo Negrete and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Nipah virus categories.
Henipavirus Assembly And Budding
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Author : Weina Sun
language : en
Publisher:
Release Date : 2015
Henipavirus Assembly And Budding written by Weina Sun and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
The emergence of a group of paramyxoviruses called henipavirus has caused fatal illness such as severe vasculitis and encephalitis which resulted in high fatality rate in both humans and animals since 1990's in south Asia and Australia. Like other paramyxoviruses, the essential viral protein that organizes the process of henipavirus assembly and budding is the matrix protein (M), where it functions to link together the viral glycoproteins and ribonucleoprotein complex (RNPs). Current studies have sought out to investigate the host factors that are involved in virus assembly and budding by interacting with M protein to facilitate M functions.In an effort to identify host factors that are important for henipavirus assembly and budding, proteomics-based approach was performed to identify host proteins that interact with viral M protein. Here, we affinity purified viral M proteins by FPLC and identified co-purifying host proteins by mass spectrometry. Co-immunoprecipitation was used as a secondary screening of protein candidates identified from mass spec results. One of the host proteins candidates, AP3B1, the beta subunit of AP-3 complex, was selected for further investigations. Importantly, a 29 amino acids polypeptide derived from AP3B1 hinge domain was identified as the minimal fragment to bind M protein as well as effectively inhibit henipavirus-like particles (VLPs) production. This inhibitory effect is due to disruption of M protein association with membrane. Additionally, in AP3B1 depleted cells by siRNA knockdown, Nipah VLP production is significantly reduced. By immunofluorescenceivmicroscopy, M protein colocalization with endogenous AP3B1 was also observed in mammalian cells. Our results suggested that AP-3 might play an important role in M protein functions.Henipaviruses have two types of surface glycoproteins, the attachment protein (G) and fusion protein (F). They were responsible for virus entry by mediating virus attachment to cell receptors and fusion with cell membrane. Numerous studies have illustrated how the virus entry occurs under the coordination of G and F proteins. However, little is known about how glycoproteins assemble into virions or VLPs. We know that M protein is the main driving force of particles formation, so we wondered how M protein coordinates with G protein and F protein to facilitate their trafficking and assembly into virions or VLPs. Here, under the unique pathway of HeV F protein identified by our collaborator Dr. Rebecca Dutch at University of Kentucky, we have found that HeV M protein, F protein and G protein all partially colocalized with Rab11a-REs, and overexpression of a DN Rab11a could significantly inhibit Hendra M-VLPs as well as F-VLPs formation in the cells. Interestingly, in cells that express inhibitory AP3B1 Hinge domain, M colocalization with Rab11-REs was disrupted. Unlike M protein or F protein, G proteins releases poorly into particles when expressed alone in cells. We observed that G incorporation into VLPs could be facilitated by co-expression of M protein but not F protein, likely through G-M interaction at the plasma membrane. Moreover, we have found that F protein incorporation into VLPs depended on its endocytic trafficking event regardless its cleavage status, as an endocytosis defective mutant F S490A that was retained onvthe cell surface was shown to be significantly defective on particles assembly. On the other hand, HeV F protein still well incorporated into VLPs when its cleavage was prevented by a cathepsin L inhibitor, E-64d. Therefore, we hypothesized that henipavirus M and F proteins have separate mechanisms for trafficking to Rab11-REs, with the M protein trafficking facilitated by its interaction with AP3B1. M and cathepsin-cleaved F proteins must then assemble together within these compartments prior to their delivery to the cell surface for particle budding and assemble with G protein through G-M interaction at the plasma membrane.
Refining The Mechanism Of Henipavirus Mediated Membrane Fusion Through Mutagenesis Of The Hendra Virus Envelope Glycoproteins
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Author : Kimberly Ann Bishop-Lilly
language : en
Publisher:
Release Date : 2007
Refining The Mechanism Of Henipavirus Mediated Membrane Fusion Through Mutagenesis Of The Hendra Virus Envelope Glycoproteins written by Kimberly Ann Bishop-Lilly and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Paramyxoviruses categories.
The Molecular Characterization Of Niv C Protein And The Identification Of Henipavirus Host Protein Interactions
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Author : Thomas S. Mccrory
language : en
Publisher:
Release Date : 2012
The Molecular Characterization Of Niv C Protein And The Identification Of Henipavirus Host Protein Interactions written by Thomas S. Mccrory and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.
