Inclusion Bodies
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Inclusion Bodies
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Author : Julian Kopp
language : en
Publisher: Springer Nature
Release Date : 2023-01-19
Inclusion Bodies written by Julian Kopp and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-01-19 with Science categories.
This detailed volume presents a series of protocols dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purification of refolded product. Commencing with chapters on upstream processing, looking into different expression strategies for IB production, the book continues with downstream applications, highlighting early protein purification and subsequent analytics, as well as success stories of IB-based processes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Inclusion Bodies: Methods and Protocols serves as an ideal resource for facilitating diverse aspects of IB processing.
The Nature Of Inclusion Bodies
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Author : Milton Levine
language : en
Publisher:
Release Date : 1936
The Nature Of Inclusion Bodies written by Milton Levine and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1936 with Inclusion bodies categories.
Physicochemical Properties Of Protein Inclusion Bodies
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Author : Norbertus Djajasantosa Wangsa-Wirawan
language : en
Publisher:
Release Date : 1999
Physicochemical Properties Of Protein Inclusion Bodies written by Norbertus Djajasantosa Wangsa-Wirawan and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Escherichia coli categories.
Improvements in the current production system of inclusion bodies and the downstream processing sequence are essential to maintain a competitive advantage in the market place. Optimisation of fermentation is considered to improve production yield; then flotation as a possible inclusion body recovery method.
Protein Aggregation In Bacteria
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Author : Silvia Maria Doglia
language : en
Publisher: John Wiley & Sons
Release Date : 2014-04-03
Protein Aggregation In Bacteria written by Silvia Maria Doglia and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-04-03 with Science categories.
Focuses on the aggregation of recombinant proteins in bacterial cells in the form of inclusion bodies—and on their use in biotechnological and medical applications The first book devoted specifically to the topic of aggregation in bacteria, Protein Aggregation in Bacteria: Functional and Structural Properties of Inclusion Bodies in Bacterial Cells provides a large overview of protein folding and aggregation, including cell biology and methodological aspects. It summarizes, for the first time in one book, ideas and technical approaches that pave the way for a direct use of inclusion bodies in biotechnological and medical applications. Protein Aggregation in Bacteria covers: Molecular and cellular mechanisms of protein folding, aggregation, and disaggregation in bacteria Physiological importance and consequences of aggregation for the bacterial cell Factors inherent to the protein sequence responsible for aggregation and evolutionary mechanisms to keep proteins soluble Structural properties of proteins expressed as soluble aggregates and as inclusion bodies within bacterial cells both from a methodological point of view and with regard to their similarity with amyloids Control of the structural and functional properties of aggregated proteins and use thereof in biotechnology and medicine Protein Aggregation in Bacteria is ideal for researchers in protein science, biochemistry, bioengineering, biophysics, microbiology, medicine, and biotechnology, particularly if they are related with the production of recombinant proteins and pharmaceutical science.
A Study Of Inclusion Bodies Found In The Human Vaginal Tract
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Author : Madathilparampil M. Thomas
language : en
Publisher:
Release Date :
A Study Of Inclusion Bodies Found In The Human Vaginal Tract written by Madathilparampil M. Thomas and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Inclusions In Prokaryotes
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Author : Jessup M. Shively
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-05-04
Inclusions In Prokaryotes written by Jessup M. Shively and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-05-04 with Science categories.
The new series "Microbiology Monographs" begins with two volumes on intracellular components in prokaryotes. In this first volume, "Inclusions in Prokaryotes", the components, labeled inclusions, are defined as discrete bodies resulting from synthesis of a metabolic product. Research on the biosynthesis and reutilization of the accumulated materials is still in progress, and interest in the inclusions is growing. This comprehensive volume provides historical background and comprehensive reviews of eight well-known prokaryotic inclusions.
Do Inclusion Bodies Occur In Poliomyelitis
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Author : L. Glynne Price
language : en
Publisher:
Release Date : 1930
Do Inclusion Bodies Occur In Poliomyelitis written by L. Glynne Price and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1930 with categories.
Inclusion Bodies In Swine Fever
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Author : J. Bogdan
language : en
Publisher:
Release Date : 1978*
Inclusion Bodies In Swine Fever written by J. Bogdan and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1978* with categories.
Inclusion Body Myositis And Myopathies
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Author : Valerie Askanas
language : en
Publisher: Cambridge University Press
Release Date : 1998-04-13
Inclusion Body Myositis And Myopathies written by Valerie Askanas and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-04-13 with Medical categories.
Inclusion-body myositis (IBM) is now understood to be an important degenerative muscle disease. The sporadic type (s-IBM) is probably the most common muscle disease among those ailments that strike first in adulthood (particularly people over 50). The hereditary type (h-IBM) affects younger patients. This book is devoted entirely to s-IBM and h-IBM. Contributors discuss what is understood about the basic scientific foundations of IBMs, the varied aspects of the pathology of IBMs, and the application of clinical treatments. One particular emphasis of the book is on the hereditary aspects of IBM and genetic predispositions to the disease.
Biomedical Applications Of Bacterial Inclusion Bodies
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Author : Joaquin Seras Franzoso
language : en
Publisher:
Release Date : 2012
Biomedical Applications Of Bacterial Inclusion Bodies written by Joaquin Seras Franzoso and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.
Els cossos d'inclusió bacterians (CIs) són dipòsits proteics que apareixen normalment durant el procés de producció de proteïna recombinant. Històricament, aquests agregats han estat classificats com a sub-productes de rebuig i, per tant, descartats. No obstant, aquesta percepció ha canviat al llarg de les dues darreres dècades. Nombrosos estudis han proporcionat evidències que demostren que aquestes partícules són, de fet, estructures supramoleculars formades per contactes estereoespecífics proteïna-proteïna, morfològicament i fisicoquímicament estables, fàcils de purificar i que mostren un alt nivell de plasticitat pel que fa a activitat biològica, mida i propietats fisicoquímiques. En el present treball s'ha explorat la possibilitat d'aprofitar les propietats úniques dels CIs per desenvolupar noves aplicacions biomèdiques. En aquest sentit, ens hem centrat en l'estudi de la idoneïtat dels CIs com a nanomaterial particulat per tal de generar nanotopografies capaces de controlar la resposta de cèl·lules animals. La modificació a nivell nanomètric de superfícies per tal d'estimular una resposta cel·lular específica resulta molt prometedora pels camps de l'enginyeria tissular i la medicina regenerativa en general. En aquest treball es presenten evidències de que els CIs es poden dipositar sobre superfícies de cultiu, obtenint nanotopografies modificades amb efecte biològic. Al cultivar cèl·lules de mamífer sobre aquestes, s'aprecia un elevada capacitat d'adhesió cel·lular. A més a més, s'ha observat que el CIs són capaços d'estimular la proliferació cel·lular mitjançant una cascada de senyalització basada en la mecanotransducció. Tant l'adhesió com la proliferació cel·lulars són factors crucials per la colonització de la superfície d'implants. S'ha demostrat també que les topografies formades per CIs són capaces de dirigir cèl·lules mare mesenquimals (MSCs) indiferenciades cap a osteoblasts. És important esmentar que l'adhesió, la proliferació i la diferenciació cel·lular mediades per CIs poden ser modulades per la correcta elecció del fons genètic de la soca utilitzada per a produir els CIs, obtenint d'aquesta manera agregats fisicoquímica i morfològicament diferents. Finalment els CIs han demostrat ser capaços d'alliberar quantitats significatives de la proteïna que els forma, en una versió activa. Aquest fet ha propiciat el seu estudi com plataforma d'entrega de fàrmacs proteics, des de nanoestructures de la superfície del substrat, cap als cultius diana. Aquesta darrera aplicació seria particularment interessant en tractaments a llarg termini, ja que el CIs proporcionarien un alliberament de la proteïna sostingut. A més, aquesta capacitat d'entrega afegiria un nivell extra de complexitat i control a les aplicacions prèviament esmentades ja que combinaria l'efecte mecànic de les topografies basades en CIs amb l'activitat biològica de la proteïna formadora de l'agregat.