Insertional Mutagenesis As A Tool For Identifying And Characterizing Novel Candidate Cancer Genes
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Insertional Mutagenesis As A Tool For Identifying And Characterizing Novel Candidate Cancer Genes
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Author : Jacquelyn Jinan Roth
language : en
Publisher:
Release Date : 2011
Insertional Mutagenesis As A Tool For Identifying And Characterizing Novel Candidate Cancer Genes written by Jacquelyn Jinan Roth and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
Cancer results from dynamic changes in a set of cellular genes. Mutations in oncogenes and tumor suppressor genes are responsible for converting a normal cell into a malignant one. Much research has been done to identify a large number of oncogenes and tumor suppressor genes that are frequently mutated in human cancers. These efforts suggest that the cancer genome is composed of a few commonly mutated genes along with hundreds of infrequently mutated genes. Deciphering which of the mutations are important in tumorigenesis will aid in understanding the biology of cancer as well as provide potential new therapeutic targets. Mouse models of human cancer have been used to determine the significance genetic alterations identified in human cancers. One tool that has been used for this purpose in the mouse is insertional mutagenesis. In our study, we designed a Sleeping Beauty transposon-based forward genetic screen in mice to identify candidate genes for breast cancer in the presence and absence of Cav1 mutations. Analysis of transposon integrations in all mammary tumors identified 210 common insertion sites (CISs) and candidate (CAN) genes were assigned to many of the CISs. Multiple CAN genes have been previously implicated in human breast cancers. Identifying the roles of these 210 CAN genes in tumorigenesis will determine their significance in human breast cancer. In this study, we also investigated the role of a transcription factor, Meis1, which was originally identified in murine leukemias caused by retroviral-induced insertional mutagenesis. Overexpression of Meis1 in conjunction with HoxA9 is detected in a variety of myeloid leukemia cell lines and primary human samples of acute myeloid leukemia (AML). In an effort to identify additional downstream targets of Meis1, we generated FDC-P1 cells overexpressing Meis1 in the presence or absence of HoxA9. Microarray analysis was performed on RNA isolated from these cells. A combination of bioinformatics and statistical analyses were used to look for genes with differential regulation in the presence or absence of Meis1 and HoxA9. These data were used to generate a genetic signature characteristic of Meis1 expression and shed light onto other pathways that may involve Meis1 and HoxA9.
Hereditary Colorectal Cancer
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Author : Laura Valle
language : en
Publisher: Springer
Release Date : 2018-05-04
Hereditary Colorectal Cancer written by Laura Valle and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-04 with Medical categories.
This book provides information on a wide variety of issues ranging from genetics to clinical description of the syndromes, genetic testing and counseling, and clinical management including surveillance, surgical and prophylactic interventions, and chemoprevention. Moreover, current hot issues, such as the identification of novel causal genes and the challenges we face, and the relevance of cancer risk modifiers, both genetic and environmental, are also discussed. This reference book is great for geneticists, oncologists, genetic counselors, researchers, clinicians, surgeons and nurses dedicated to, or interested in, hereditary cancer. The best and most recognized experts in the field have contributed to this project, guaranteeing updated information, accuracy and the discussion of topical issues.
Gene Therapy For Cancer
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Author : Kelly K. Hunt
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-26
Gene Therapy For Cancer written by Kelly K. Hunt and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-26 with Medical categories.
The three sections of this volume present currently available cancer gene therapy techniques. Part I describes the various aspects of gene delivery. In Part II, the contributors discuss strategies and targets for the treatment of cancer. Finally, in Part III, experts discuss the difficulties inherent in bringing gene therapy treatment for cancer to the clinic. This book will prove valuable as the volume of preclinical and clinical data continues to increase.
How Tobacco Smoke Causes Disease
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Author :
language : en
Publisher:
Release Date : 2010
How Tobacco Smoke Causes Disease written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Government publications categories.
This report considers the biological and behavioral mechanisms that may underlie the pathogenicity of tobacco smoke. Many Surgeon General's reports have considered research findings on mechanisms in assessing the biological plausibility of associations observed in epidemiologic studies. Mechanisms of disease are important because they may provide plausibility, which is one of the guideline criteria for assessing evidence on causation. This report specifically reviews the evidence on the potential mechanisms by which smoking causes diseases and considers whether a mechanism is likely to be operative in the production of human disease by tobacco smoke. This evidence is relevant to understanding how smoking causes disease, to identifying those who may be particularly susceptible, and to assessing the potential risks of tobacco products.
Pharmaceutical Biotechnology
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Author : Daan J. A. Crommelin
language : en
Publisher: CRC Press
Release Date : 2002-11-14
Pharmaceutical Biotechnology written by Daan J. A. Crommelin and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-11-14 with Medical categories.
The field of pharmaceutical biotechnology is evolving rapidly. A whole new arsenal of protein pharmaceuticals is being produced by recombinant techniques for cancer, viral infections, cardiovascular and hereditary disorders, and other diseases. In addition, scientists are confronted with new technologies such as polymerase chain reactions, combinatorial chemistry and gene therapy. This introductory textbook provides extensive coverage of both the basic science and the applications of biotechnology-produced pharmaceuticals, with special emphasis on their clinical use. Pharmaceutical Biotechnology serves as a complete one-stop source for undergraduate pharmacists, and it is valuable for researchers and professionals in the pharmaceutical industry as well.
Scientific Frontiers In Developmental Toxicology And Risk Assessment
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Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 2000-12-21
Scientific Frontiers In Developmental Toxicology And Risk Assessment written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-12-21 with Nature categories.
Scientific Frontiers in Developmental Toxicology and Risk Assessment reviews advances made during the last 10-15 years in fields such as developmental biology, molecular biology, and genetics. It describes a novel approach for how these advances might be used in combination with existing methodologies to further the understanding of mechanisms of developmental toxicity, to improve the assessment of chemicals for their ability to cause developmental toxicity, and to improve risk assessment for developmental defects. For example, based on the recent advances, even the smallest, simplest laboratory animals such as the fruit fly, roundworm, and zebrafish might be able to serve as developmental toxicological models for human biological systems. Use of such organisms might allow for rapid and inexpensive testing of large numbers of chemicals for their potential to cause developmental toxicity; presently, there are little or no developmental toxicity data available for the majority of natural and manufactured chemicals in use. This new approach to developmental toxicology and risk assessment will require simultaneous research on several fronts by experts from multiple scientific disciplines, including developmental toxicologists, developmental biologists, geneticists, epidemiologists, and biostatisticians.
Genome Editing And Engineering
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Author : Krishnarao Appasani
language : en
Publisher: Cambridge University Press
Release Date : 2018-08-23
Genome Editing And Engineering written by Krishnarao Appasani and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-08-23 with Science categories.
A complete guide to endonuclease-based genomic engineering, from basic science to application in disease biology and clinical treatment.
Cancer Gene Therapy
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Author : David T. Curiel
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-11-03
Cancer Gene Therapy written by David T. Curiel and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-03 with Medical categories.
A complete introduction and guide to the latest developments in cancer gene therapy-from bench to bedside. The authors comprehensively review the anticancer genes and gene delivery methods currently available for cancer gene therapy, including the transfer of genetic material into the cancer cells, stimulation of the immune system to recognize and eliminate cancer cells, and the targeting of the nonmalignant stromal cells that support their growth. They also thoroughly examine the advantages and limitations of the different therapies and detail strategies to overcome obstacles to their clinical implementation. Topics of special interest include vector-targeting techniques, the lessons learned to date from clinical trials of cancer gene therapy, and the regulatory guidelines for future trials. Noninvasive techniques to monitor the extent of gene transfer and disease regression during the course of treatment are also discussed.
Safety Of Genetically Engineered Foods
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Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 2004-07-08
Safety Of Genetically Engineered Foods written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-07-08 with Science categories.
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
Plant Functional Genomics
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Author : Jose M. Alonso
language : en
Publisher: Humana
Release Date : 2016-10-05
Plant Functional Genomics written by Jose M. Alonso and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-05 with Science categories.
This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model systems. This book is organized into five parts. Part I examines the tools and methods required for identifying epigenetic and conformational changes at the whole-genome level. Part II presents approaches used to determine key aspects of a gene’s function, such as techniques used to identify and characterize gene regulatory networks. This is followed by a discussion of tools used to analyze the levels of mRNA, mRNA translation rates and metabolites. Part III features a compilation of forward and reverse genetic approaches that include recent implementation of high-throughput sequencing in classical methodologies such as QTL mapping. The final two parts explore strategies to facilitate and accelerate the generation and testing of functional DNA elements and basic computational tools used to facilitate the use of systems biology approached by a broad spectrum of plant researchers. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Practical and timely, Plant Functional Genomics: Methods and Protocols, Second Edition highlights the latest developments in DNA sequencing technologies that are likely to continue shaping the future of functional genomics.