Intravital Microscopy Imaging Of Leukocytes
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Intravital Microscopy Imaging Of Leukocytes
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Author : Elzbieta Kolaczkowska
language : en
Publisher: Frontiers Media SA
Release Date : 2020-12-01
Intravital Microscopy Imaging Of Leukocytes written by Elzbieta Kolaczkowska and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-12-01 with Medical categories.
This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.
Tracking Colliding Cells In Intravital Video Microscopy
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Author : Nhat Huynh Khanh Nguyen
language : en
Publisher:
Release Date : 2009
Tracking Colliding Cells In Intravital Video Microscopy written by Nhat Huynh Khanh Nguyen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with categories.
Leukocyte motion represents an important component in the innate immune re¬sponse to infection. Intravital microscopy is a powerful tool as it enables in vivo imaging of leukocyte motion. In inflammatory conditions, leukocytes may exhibit various motion behaviors such as flowing, rolling, and adhering. With many cells moving at a variety of speeds, collisions occur. These collisions result in abrupt changes in the motion and appearance of leukocytes. Analyzing the motion behavior of leukocytes is a difficult task. Manual analysis is tedious, error-prone, and could introduce technician-related bias. Automatic tracking methods are also challenging due to the noise inherent in the biomedical imaging process, varied cell appearance during collision, and abrupt change in cell motion patterns. This work presents a novel method to automatically track multiple cells undergoing collisions by modeling the collision states of cells and testing multiple hypotheses of their motion and ap¬pearance. The tracking results are demonstrated using in vivo intravital microscopy image sequences. We have found that: (1) the proposed method tracks 32% more cells than a previous approach without introducing any additional position error; (2) the improvement of the proposed method is emphasized when the duration of collision increases; and (3) given good detection results, the proposed method can correctly track 88% of the total number of cells.
Intravital Imaging Of Dynamic Bone And Immune Systems
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Author : Masaru Ishii
language : en
Publisher: Humana
Release Date : 2019-06-06
Intravital Imaging Of Dynamic Bone And Immune Systems written by Masaru Ishii and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-06 with Medical categories.
This detailed volume presents a number of chapters that will aid researchers in jumping the hurdle preventing the popularization of advanced intravital imaging technology usage, namely the complicated experimental protocols. Leading researchers undertaking imaging studies in the field of bone and immune systems have contributed the chapters that aim to guide readers through these complex methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Intravital Imaging of Dynamic Bone and Immune Systems: Methods and Protocols will serve as an idea guide for general readers in order to establish and perform these experiments by their own.
Intravital Imaging Of Dynamic Behaviors Of Leukocytes In Uvb Induced Skin Inflammation
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Author : Ran Lu
language : en
Publisher:
Release Date : 2013
Intravital Imaging Of Dynamic Behaviors Of Leukocytes In Uvb Induced Skin Inflammation written by Ran Lu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.
Ultraviolet B (UVB) irradiation diminishes surface densities of Langerhans cells (LCs) in the epidermis of the skin. Other leukocytes, such as neutrophils and macrophages, are recruited to the irradiated sites due to chemo-attracting factors released from the altered microenvironment. However, there is little evidence directly demonstrating the behavior of LCs as well as other leukocytes in response to UVB radiation, and it is also unknown whether cellular interaction occurs during skin inflammation. In the present study, we developed a direct visualization system to observe the dynamic behaviors of both LCs and neutrophils responding to 3000J/m2 UVB in the skin. Using intravital confocal microscopy and genetically manipulated transgenic mice, we have found that neutrophils are recruited to inflammatory sites immediately after UVB irradiation. Skin-infiltrating neutrophils can interact with LCs upon activation and can last for several hours. Twenty-four hours after irradiation, LCs migrated to deeper skin layers, resulting in a 30% reduction of LC number in the epidermal compartment, whereas the number of recruited neutrophils increased over time. LCs completely disappeared from the epidermis 4 days after UVB treatment. Depletion of neutrophils by anti-Ly6G monoclonal antibody (mAb) administration did not affect LCs migration in response to the same UVB radiation, but rather promoted LC precursors' repopulation to the epidermis after UVB treatment. In addition, depletion of neutrophils greatly diminished the magnitude of UVB-induced ear swelling, suggesting that neutrophils are crucial for UVB induced skin inflammation. Not only do our data unveil the behavior and cell-cell interaction of LCs and neutrophils upon UVB irradiation, they also indicate for the first time that neutrophils play an important role in LC repopulation.
Inflammation In The Cns Advancing The Field Using Intravital Imaging
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Author : Saparna Pai
language : en
Publisher: Frontiers Media SA
Release Date : 2017-12-20
Inflammation In The Cns Advancing The Field Using Intravital Imaging written by Saparna Pai and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-20 with Electronic book categories.
Inflammation of the CNS can have devastating, long-lived, and in some cases fatal consequences for patients. The stimuli that can induce CNS inflammation are diverse, and include infectious agents, autoimmune responses against CNS-expressed antigens, and sterile inflammation following ischemia or traumatic injury. In these conditions, cells of the immune system play central roles in promulgation and resolution of the inflammatory response. However, the immunological mechanisms at work in these diverse responses differ according to the nature of the response. Our understanding of the actions of immune cells in the CNS has been restricted by the difficulty in visualising leukocytes as they undergo recruitment from the cerebral microvasculature and following their entry into the CNS parenchyma. However, advances in in vivo microscopy over the last 10-15 years have overcome many of these difficulties, and studies using these forms of microscopy have revealed a wealth of new information regarding the cellular and molecular mechanisms of CNS inflammation. This Research Topic brings together state of the art reviews examining the use of in vivo imaging in investigating inflammation and leukocyte behaviour in the CNS. Papers in this Research Topic describe how in vivo microscopy has increased our understanding of the actions of immune cells in the inflamed CNS, following various stimuli including autoimmunity, infection and sterile inflammation.
Detection Of Rolling Leukocytes From Intravital Microscopy Images
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Author : Gang Dong
language : en
Publisher:
Release Date : 2006
Detection Of Rolling Leukocytes From Intravital Microscopy Images written by Gang Dong and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.
Visualizing Immunity
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Author : Dorian McGavern
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-06-12
Visualizing Immunity written by Dorian McGavern and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-06-12 with Medical categories.
Researchers have used a variety of techniques over the past century to gain fun- mental insights in the field of immunology and, as technology has advanced, so too has the ability of researchers to delve deeper into the biological mechanics of immunity. The immune system is exceedingly complex and must patrol the entire body to protect us from foreign invaders. This requires the immune system to be highly mobile and adaptable - able to respond to diverse microbial challenges while maintaining the ability to distinguish self from a foreign invader. This latter feature is of great importance because the immune system is equipped with toxic mediators, and a failure in self/non-self discrimination can result in serious diseases. Fortunately, in most cases, the immune system operates within the framework of its elegant design and protects us from diverse microbial challenges without initiating disease. Because the immune system is not confined to a single tissue, a comprehensive understanding of immunity requires that research be conducted at the molecular, cellular, and systems level. Immune cells often find customized solutions to h- dling microbial insults that depend on the tissue(s) in which the pathogen is found.
Imaging And Mechanism Of Leukocyte Recruitment And Function In Inflammation And Infections
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Author : Zhichao Fan
language : en
Publisher: Frontiers Media SA
Release Date : 2021-07-05
Imaging And Mechanism Of Leukocyte Recruitment And Function In Inflammation And Infections written by Zhichao Fan and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-07-05 with Science categories.
Make Life Visible
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Author : Yoshiaki Toyama
language : en
Publisher: Springer Nature
Release Date : 2019-10-02
Make Life Visible written by Yoshiaki Toyama and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-02 with Medical categories.
This open access book describes marked advances in imaging technology that have enabled the visualization of phenomena in ways formerly believed to be completelyimpossible. These technologies have made major contributions to the elucidation of the pathology of diseases as well as to their diagnosis and therapy. The volume presents various studies from molecular imaging to clinical imaging. It also focuses on innovative, creative, advanced research that gives full play to imaging technology inthe broad sense, while exploring cross-disciplinary areas in which individual research fields interact and pursuing the development of new techniques where they fuse together. The book is separated into three parts, the first of which addresses the topic of visualizing and controlling molecules for life. Th e second part is devoted to imaging of disease mechanisms, while the final part comprises studies on the application of imaging technologies to diagnosis and therapy. Th e book contains the proceedings of the 12th Uehara International Symposium 2017, “Make Life Visible” sponsored by the Uehara Memorial Foundation and held from June 12 to 14, 2017. It is written by leading scientists in the field and is an open access publication under a CC BY 4.0 license.
Streptococcus Pneumoniae
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Author : Federico Iovino
language : en
Publisher: Humana Press
Release Date : 2019-04-01
Streptococcus Pneumoniae written by Federico Iovino and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-01 with Medical categories.
This volume looks at the newest methods, materials, equipment, and technologies developed to study the cell biology of the pneumococcus. The chapters, which cover a wide range of topics, are organized into six parts: Part one discusses the cultivation of Streptococcus pneumoniae in vitro; Part Two talks about the microscopy techniques used to study the biology of Streptococcus pneumoniae and pneumococcal interactions with the host; Parts Three and Four describe the genetics, proteome, and proteomics of Streptococcus pneumoniae; Part Five explains in vitro and in vivo models of Streptococcus pneumoniae-host interactions; and Part Six looks at the factors of public health, epidemiology, and biostatistics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Streptococcus pneumoniae: Methods and Protocols is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in this continuously evolving field.