Isolation And Characterization Of Atlantic Salmon Salmo Salar Olfactory Receptor Genes

DOWNLOAD

Download Isolation And Characterization Of Atlantic Salmon Salmo Salar Olfactory Receptor Genes PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Isolation And Characterization Of Atlantic Salmon Salmo Salar Olfactory Receptor Genes book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page

Isolation And Characterization Of Atlantic Salmon Salmo Salar Olfactory Receptor Genes

DOWNLOAD
Author : Jill Elizabeth Thorpe
language : en
Publisher:
Release Date : 2000

Isolation And Characterization Of Atlantic Salmon Salmo Salar Olfactory Receptor Genes written by Jill Elizabeth Thorpe and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Atlantic salmon categories.

Identification Evolution And Expression Of Three Olfactory Gene Families In Atlantic Salmon Salmo Salar

DOWNLOAD
Author : Kimberley Anne Johnstone
language : en
Publisher:
Release Date : 2011

Identification Evolution And Expression Of Three Olfactory Gene Families In Atlantic Salmon Salmo Salar written by Kimberley Anne Johnstone and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Atlantic salmon categories.

It has been hypothesized that salmonids use olfactory cues to return to their natal rivers and streams. However, the key components of the molecular pathway involved in imprinting and homing are still unknown. If odorants are involved in salmon homing migration then olfactory receptors should play a critical role in the dissipation of information from the environment to the fish. To understand the molecular basis for imprinting and homing in Atlantic salmon (Salmo salar), it is important to identify and characterize the olfactory receptors in the Atlantic salmon genome. Aquatic chemical cues are received through the salmon nares and into the nasal cavity that contains a single olfactory organ, olfactory rosette. The olfactory rosette contains sensory neurons, which are thought to express only one olfactory receptor. In this study, three major superfamilies of fish olfactory receptors (MOR, ora and OlfC) were examined. To identify the olfactory genes in Atlantic salmon several genomic and bioinformatic techniques were used. First, an Atlantic salmon bacterial artificial chromosome (BAC) library was screened with probes designed from previously identified fish olfactory receptor sequences. Then a selected number of hybridization positive BACs containing olfactory receptors were shotgun cloned and sequenced. From these BAC sequences, two ora genes and 55 OlfC genes were identified in Atlantic salmon. The second technique used to identify olfactory receptors in Atlantic salmon was a bioinformatic approach that involved screening a 3-fold Atlantic salmon genome sequence for olfactory receptors. Using this approach, 24 MOR and the remaining five ora genes were identified, as well as another 24 partial genes or pseudogenes. As a first step to understand how olfactory receptors are involved in imprinting and homing, a suite of olfactory receptors were selected to examine the expression profiles of these genes across different life stages and life histories of wild Atlantic salmon from Newfoundland, Canada. Seven differentially expressed OlfC genes were identified in juvenile anadromous salmon compared to returning adult salmon. From this research, I hypothesize that OlfC genes may play an important role in the imprinting of home stream water olfactory cues in anadromous Atlantic salmon.

Isolation Characterization And Genetic Analysis Of Simple Sequence Length Polymorhism In Atlantic Salmon Salmo Salar

DOWNLOAD
Author : Audun Slettan
language : en
Publisher:
Release Date : 1995

Isolation Characterization And Genetic Analysis Of Simple Sequence Length Polymorhism In Atlantic Salmon Salmo Salar written by Audun Slettan and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.

Identification Evolution And Expression Of The Trace Amine Associated Receptor Taar Gene Family In Atlantic Salmon Salmo Salar

DOWNLOAD
Author : Jordan Anthony Tessarolo
language : en
Publisher:
Release Date : 2014

Identification Evolution And Expression Of The Trace Amine Associated Receptor Taar Gene Family In Atlantic Salmon Salmo Salar written by Jordan Anthony Tessarolo and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.

It is widely hypothesized that Atlantic salmon are imprinted at a young age with olfactory cues, which they use as a guide in order to return to their natal streams to spawn. However, the molecular mechanism(s) behind this biological phenomenon remain unknown. Therefore, in order to better understand imprinting and homing in Atlantic salmon, it is important to characterize the repertoire of olfactory receptors in thisspecies. A search of the first assembly of the Atlantic salmon genome revealed 27 putatively functional trace amine-associated receptor (TAAR) genes and 25 putative TAAR pseudo-genes. Genetic mapping, phylogenetic analysis, binding-site prediction, and quantitative PCR were performed using the Atlantic salmon TAAR genes. The identification of this gene family in Atlantic salmon will facilitate additional studiesinvolving olfaction and homing such as determining the range of allelic variation in olfactory receptors genes of different salmon populations.

Isolation And Characterization Of The Carotenoid Binding Protein In Atlantic Salmon Salmo Salar

DOWNLOAD
Author : Michelle Côté
language : en
Publisher:
Release Date : 2000

Isolation And Characterization Of The Carotenoid Binding Protein In Atlantic Salmon Salmo Salar written by Michelle Côté and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with categories.

The Characterization Of Repetitive Dna Elements In The Genome Of Atlantic Salmon Salmo Salar

DOWNLOAD
Author : John L. Goodier
language : en
Publisher:
Release Date : 1994

The Characterization Of Repetitive Dna Elements In The Genome Of Atlantic Salmon Salmo Salar written by John L. Goodier and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Atlantic salmon categories.

Cloning And Expression Analysis Of Atlantic Salmon Salmo Salar Cyp1a

DOWNLOAD
Author : Christopher Benjamin Rees
language : en
Publisher:
Release Date : 2001

Cloning And Expression Analysis Of Atlantic Salmon Salmo Salar Cyp1a written by Christopher Benjamin Rees and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Atlantic salmon categories.

The Isolation And Characterization Of The Light Antibody From Atlantic Salmon Salmo Salar

DOWNLOAD
Author : D. H. Davies
language : en
Publisher:
Release Date : 1975

The Isolation And Characterization Of The Light Antibody From Atlantic Salmon Salmo Salar written by D. H. Davies and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1975 with categories.

Identification And Annotation Of Full Length Genes In Atlantic Salmon Salmo Salar

DOWNLOAD
Author : Jong S. Leong
language : en
Publisher:
Release Date : 2010

Identification And Annotation Of Full Length Genes In Atlantic Salmon Salmo Salar written by Jong S. Leong and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Atlantic salmon categories.

Large-scale expressed sequence tags (ESTs) in Atlantic salmon (Salmo salar) are examined to answer questions regarding salmonid transcriptomes. ESTs represent raw and incomplete gene sequences that need to be read, assembled and analyzed with computer software. The goal of this thesis was to develop an automatically curated and publicly accessible set of annotated full-length genes, representing a near-complete transcript set for Salmo salar. In turn, these genes provide the framework for studies in gene expression, conservation, and molecular evolution. The work presented here also touches on the results of a molecular evolution study, as an example of how full-length gene identification can be used to answer biological questions. Previous to this study, a limited number of Atlantic salmon cDNA libraries and ESTs were known. To further the goal of determining complete gene sequences, highly enriched full-length cDNA libraries and full-length libraries were created and sequenced, resulting in the ability to identify a large number of full-length reference genes. Together, all libraries represent a diverse pool of transcriptome sequences for Salmo salar. The goal of producing an accurate large-scale full-length gene set on a duplicated genome is not trivial. Complete systems for this objective do not readily exist. EST sequencing, EST assembly, and data storage, are just a few of the initial computational issues that are addressed. Once these issues are resolved, the multi-step workflow of full-length gene determination is described. The final challenge involving the development of a concise and universally accessible system for visualization is discussed. The resulting computational framework that has been developed is shown to be able to handle the intricacies and the size of a duplicated salmonid genome. It has been largely accepted that Atlantic salmon have undergone a recent genome duplication. Gene paralogs provide one source of evidence for this event. Analysis of paralogs revealed signatures of asymmetric evolution possibly due to relaxation of selective pressure. This thesis provides a complete Bioinformatics analysis pipeline to analyze and to visualize a set of full-length reference genes for Atlantic salmon. Using full-length genes as a framework, the topic of molecular evolution was addressed to show evidence of asymmetrical evolution among gene duplicates. The full-length reference genes, along with ESTs and all putative transcripts, have been made publicly available. These results serve as a valuable genomic resource for next-generation sequencing and for all other salmonid research endeavours.

Technical Report

DOWNLOAD
Author :
language : en
Publisher:
Release Date : 2003

Technical Report written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Fish culture categories.