Isolation And Characterization Of Vacuolar Atpase Subunits A And C From The Midgut And Malpighian Tubules Of Mosquito Aedes Aegypti
DOWNLOAD
Download Isolation And Characterization Of Vacuolar Atpase Subunits A And C From The Midgut And Malpighian Tubules Of Mosquito Aedes Aegypti PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Isolation And Characterization Of Vacuolar Atpase Subunits A And C From The Midgut And Malpighian Tubules Of Mosquito Aedes Aegypti book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Isolation And Characterization Of Vacuolar Atpase Subunits A And C From The Midgut And Malpighian Tubules Of Mosquito Aedes Aegypti
DOWNLOAD
Author : Phillip Bin Chu
language : en
Publisher:
Release Date : 1997
Isolation And Characterization Of Vacuolar Atpase Subunits A And C From The Midgut And Malpighian Tubules Of Mosquito Aedes Aegypti written by Phillip Bin Chu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Adenosine triphosphatase categories.
Genome Wide Survey And Molecular Characterization Of Vacuolar Atpase Subunit Genes In The Yellow Fever Mosquito Aedes Aegypti Diptera Culicidae
DOWNLOAD
Author : Basak Coskun
language : en
Publisher:
Release Date : 2017
Genome Wide Survey And Molecular Characterization Of Vacuolar Atpase Subunit Genes In The Yellow Fever Mosquito Aedes Aegypti Diptera Culicidae written by Basak Coskun and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
The yellow fever mosquito, Aedes aegypti, is a significant vector of several viral diseases, including Zika, dengue fever, yellow fever, and chikungunya. Since vaccines are not currently available for these viruses, control of the disease vectors by using insecticides is the most common practice for preventing disease. As a result, Ae. aegypti has developed resistance against many of the most commonly used insecticides, including organophosphates and pyrethroids. The rise in resistance in vector mosquitoes requires the search for new control strategies, such as RNA interference (RNAi), to manage mosquito populations. Vacuolar H[sup plus]+-ATPase (V-ATPase), a multi-subunit enzyme involved in many cellular processes, including membrane energization, acidification of organelles, and entry of dengue virus into the cytoplasm, is a potential target for RNAi, though little is known about its genetic structure or expression patterns in Ae. aegypti. In this study, I performed genome-wide surveys to identify the genes encoding different subunits of the V-ATPase protein complex, partially characterized the molecular properties and expression patterns of selected V-ATPase subunit genes, and tested the feasibility of using oral-based delivery of nanoparticles formed from double-stranded RNA (dsRNA) and chitosan to suppress the expression of selected V-ATPase subunit genes in Ae. aegypti. My genome-wide surveys revealed that Ae. aegypti V-ATPase consists of 13 different subunits (A, B, C, D, E, F, G, H, a, c, c", d, e) encoded by 14 genes. Analysis of exon-intron arrangements for each gene demonstrated that each V-ATPase subunit gene has between one (subunit c) and 12 (subunit C) exons, with most genes (11) having 3 to 6 exons. Subsequent phylogenetic analysis of the deduced amino acid sequences of each subunit showed that V-ATPase subunits A, B, C, F, G, H, and a exhibited high levels of conservation among all the examined species, but subunits D, E, c, c", d, and e showed high conservation only among dipteran species. Analysis of the expression profiles in different tissues and developmental stages of three specific V-ATPase subunits (A, D, and H) showed that whereas the expression of these genes varied between tissues and developmental stages, the patterns of expression of subunits A, D, and H were very similar. The highest mRNA expression level was observed in Malpighian tubules in fourth-instar larvae. Interestingly, expression of subunits A, D, or H in different tissues of adults was highest in male hindgut versus Malpighian tubules in females. Feeding mosquito larvae with chitosan nanoparticles made with dsRNA complementary to subunits A, D, or H resulted in significant suppression of mRNA transcript levels of each of these subunits. Peak suppression of V-ATPase A, D, or H transcripts occurred on the fifth day, where the gene transcript level was suppressed by 66.0, 27.3, or 70.4%, respectively, as compared with those of the control. Additionally, feeding of dsRNA/chitosan nanoparticles targeting subunit D caused mortality starting on day 3, with cumulative larval mortality reaching 14.8% on the sixth day. These results suggest that oral delivery of dsRNA/chitosan nanoparticles can substantially suppress target gene expression in Ae. aegypti larvae. However, increasing RNAi efficiency in targeting V-ATPase subunit genes in mosquito larvae appears to be necessary in order to obtain higher larval mortality using oral delivery of dsRNA/chitosan nanoparticles.
Osmotic And Ionic Regulation
DOWNLOAD
Author : David H. Evans
language : en
Publisher: CRC Press
Release Date : 2008-11-18
Osmotic And Ionic Regulation written by David H. Evans and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-18 with Medical categories.
In the 40 years since the classic review of osmotic and ionic regulation written by Potts and Parry, there has been astonishing growth in scientific productivity, a marked shift in the direction and taxonomic distribution of research, and amazing changes in the technology of scientific research" It is indicative of the growth of the subject that as
Journal Of Experimental Biology
DOWNLOAD
Author :
language : en
Publisher:
Release Date : 2006
Journal Of Experimental Biology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Biology categories.
Identification And Characterization Of A Cd98 Heavy Chain Associated Amino Acid Transporter From Mosquito Aedes Aegypti
DOWNLOAD
Author : Xiangyang Jin
language : en
Publisher:
Release Date : 2001
Identification And Characterization Of A Cd98 Heavy Chain Associated Amino Acid Transporter From Mosquito Aedes Aegypti written by Xiangyang Jin and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Aedes aegypti categories.
Genetics Abstracts
DOWNLOAD
Author :
language : en
Publisher:
Release Date : 1998
Genetics Abstracts written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Genetics categories.
The Journal Of Experimental Biology
DOWNLOAD
Author :
language : en
Publisher:
Release Date : 2007-05
The Journal Of Experimental Biology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-05 with Biology categories.
Cumulated Index Medicus
DOWNLOAD
Author :
language : en
Publisher:
Release Date : 1998
Cumulated Index Medicus written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Medicine categories.
Advances In Insect Physiology
DOWNLOAD
Author : Jerome Casas
language : en
Publisher: Academic Press
Release Date : 2008-11-21
Advances In Insect Physiology written by Jerome Casas and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-21 with Science categories.
Advances in Insect Physiology publishes eclectic volumes containing important, comprehensive and in-depth reviews on all aspects of insect physiology. It is an essential reference source for invertebrate physiologists and neurobiologists, entomologists, zoologists and insect biochemists. First published in 1963, the serial is now edited by Steve Simpson and Jerome Casas to provide an international perspective. * Contributions from the leading researchers in entomology * Discusses the physiological diversity in insects * Includes in-depth reviews with valuable information for a variety of entomology disciplines
Review Of Medical And Veterinary Entomology
DOWNLOAD
Author :
language : en
Publisher:
Release Date : 1998
Review Of Medical And Veterinary Entomology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Insects as carriers of disease categories.