Membrane Protein Assembly
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Membrane Protein Assembly
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Author : Gunnar von Heijne
language : en
Publisher: R. G. Landes
Release Date : 1997
Membrane Protein Assembly written by Gunnar von Heijne and has been published by R. G. Landes this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Science categories.
Protein Export And Membrane Biogenesis
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Author : R.E. Dalbey
language : en
Publisher: Elsevier
Release Date : 1995-08-31
Protein Export And Membrane Biogenesis written by R.E. Dalbey and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-08-31 with Science categories.
The incentive for putting together Volume 4 of this series was to review the wealth of new information that has become available in prokaryotic organisms in protein export and membrane biogenesis. Just in the last several years, protein translocation has now been efficiently reconstituted using defined components and the mechanism by which proteins are moved across membrane bilayers is now being examined at a higher resolution. In addition, because of a new technical breakthrough using osmolytes, it is now possible to reconstitute a number of channel proteins, ATPase, receptors, and transporters. In many cases, it is possible to successfully predict the membrane topology of these types of proteins using both "hydrophobicity analysis" and the "positive inside" rule. In this volume, two chapters focus on protein translocation across membranes (Biochemical Analyses of Components Comprising the Protein Translocation Machinery of E. Coli; Protein Translocation Genetics), while several others on how proteins assemble into the ineer membrane of E. Coli (Membrane Protein Assembly; Membrane Insertion of Small Proteins: Evolutionary and Functional Aspects; Pigment-Protein Complex Assembly in Rhodobacter sphaeroides and Rhodobacter Capsulatus). Other sections review recent progress on transporters (Identification and Reconstitution of Anion Exchange Mechanisms in Bacteria; Helic Packing in the C-Terminal Half of Lactose Permease) and signal transduction (Mechanism of Transmembrane Signaling in Osmoregulation) as well as the assembly of prints into the outer membrane (Export and Assembly of Outer Membrane Proteins in E. coli). Although the emphasis of the book is on proteins, the role of phospholipids in controlling various cell surface processes is reviewed (Role of Phospholipids in coli Cell Function). I should point out the reason for the rapid progress in bacteria research is because of the possibility to apply biochemistry and genetics in this organism.
Use Of Beta Lactamase Fusions To Study Membrane Protein Assembly And Protein Export In Escherichia Coli
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Author : Yianbiao Zhang
language : en
Publisher:
Release Date : 1991
Use Of Beta Lactamase Fusions To Study Membrane Protein Assembly And Protein Export In Escherichia Coli written by Yianbiao Zhang and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with categories.
Use Of Beta Lactamase Fusions To Study Membrane Protein Assembly And Protein Export In Escherichia Coli
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Author :
language : en
Publisher:
Release Date : 1991
Use Of Beta Lactamase Fusions To Study Membrane Protein Assembly And Protein Export In Escherichia Coli written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with categories.
A genetic system for directly expressing proteins in E. coli and assessing their ability to insert into the bacterial cytoplasmic membrane was developed, consisting of a direct expression vector, and a mature P-lactamase cassette plasmid for generating translational fusions of P-lactamase to the bacterially expressed proteins. This system was used to analyse the fate of three different eukaryotic plasma membrane proteins in E. coli. The ampicillin resistance/sensitivity phenotypes of the P-lactamase fusion proteins revealed that the P-subunit of the sheep-kidney Na,K-ATPase, a class II membrane protein, inserts into the cytoplasmic membrane of E. coli, with the same topology as in its native membrane. The Alzheimer's disease amyloid A4 protein precursor, a class I membrane protein, was also found to insert into the bacterial cytoplasmic membrane, and, although preliminary, the results were compatible with its correct topological insertion into the bacterial cytoplasmic membrane. In contrast, the P2-adrenergic receptor, a complex membrane protein that has an extracellular amino-terminal domain and seven transmembrane segments, was found to misinsert into the E. coli cytoplasmic membrane. A plasmid vector for cloning sequences able to function as cytoplasmic membrane translocators of P-lactamase in E. coli was also developed. Short DNA fragments of essentially random sequence were cloned, and recombinants with functional translocators were directly selected. The spectrum of random sequences able to function as bacterial cytoplasmic membrane translocators suggests that the hydrophobicity requirements for translocation across the bacterial cytoplasmic membrane and yeast endoplasmic reticulum membrane may be more similar than previously assumed.In the above studies new and widely applicable uses of P-lactamase fusion technology have been described. As applied in this thesis they highlight similarities and differences between the mechanisms by which proteins assemble into, or are translocated across, the bacterial cytoplasmic membrane and the eukaryotic endoplasmic reticulum membrane.
Local Structural Motifs Involved In Helical Membrane Protein Assembly And Dynamic Function
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Author : Ilan Samish
language : en
Publisher:
Release Date : 2006
Local Structural Motifs Involved In Helical Membrane Protein Assembly And Dynamic Function written by Ilan Samish and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.
Protein Lipid And Membrane Traffic
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Author : North Atlantic Treaty Organization. Scientific Affairs Division
language : en
Publisher: IOS Press
Release Date : 2000
Protein Lipid And Membrane Traffic written by North Atlantic Treaty Organization. Scientific Affairs Division and has been published by IOS Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Biological transport categories.
This text concentrates on the following specific topics: the dynamic character of lipids and proteins in biological membranes; the existence of specific domains in membranes including their visualisation; the molecular mechanisms of intracellular transport of membrane constituents and the involvement of lipid-protein interactions in these processes; protein assembly; structure and folding and transport through membranes; and the intracellular sorting and targeting of individual membrane components as well as different organelles."
Studies On The Membrane Protein Assembly In Escherichia Coli
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Author : Guoqing Cao
language : en
Publisher:
Release Date : 1995
Studies On The Membrane Protein Assembly In Escherichia Coli written by Guoqing Cao and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.
Structural Biochemistry Of The Tol Pal Inner Membrane Protein Assembly
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Author : Cara Press
language : en
Publisher:
Release Date : 2022
Structural Biochemistry Of The Tol Pal Inner Membrane Protein Assembly written by Cara Press and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with categories.
Membrane Protein Structure
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Author : Stephen H White
language : en
Publisher: Springer
Release Date : 2013-05-27
Membrane Protein Structure written by Stephen H White and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-05-27 with Science categories.
Studies of receptors, ion channels, and other membrane proteins require a solid understanding of the structural principles of these important biomolecules. Membrane protein structure is, however, a very challenging field. The structures of only three types of transmembrane proteins have been determined to moderate or high resolution during the last two decades, a period during which the amino acid sequences of hundreds, if not thousands, of membrane proteins have been reported. As a result, the creation of structural models to serve as guides for studies of receptors, channels, and other membrane proteins has become crucially important. This book has been assembled in order to share the experiences and findings of expert researchers in protein structure and structure-prediction methods as well as membrane biophysics and lipid physical chemistry, whose work establishes the basis for the development of suitable model structures. The reviews presented here emphasize fundamental ideas and provide an entry to the diverse and complex literature. The four major sections deal with the general nature of the membrane protein structure problem, biochemical and molecular biological approaches to protein topology, direct structural methods, and model and physicochemical approaches. The work will be of interest to physiologists, cellular and molecular biologists, biophysicists, and biochemists working on the function of membrane proteins such as receptors, ion channels, and transporters, as well as senior graduate students and independent investigators.
A Study Of Various Components Influencing Outer Membrane Protein Assembly In Escherichia Coli K 12
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Author : Michael Wilson Laird
language : en
Publisher:
Release Date : 1996
A Study Of Various Components Influencing Outer Membrane Protein Assembly In Escherichia Coli K 12 written by Michael Wilson Laird and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with Cell membranes categories.