Metalloprotein Engineering Using Heme Protein Scaffolds To Investigate The Oxidation Of Endogenous Aromatic Amino Acids And Exogenous Substrates
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Metalloprotein Engineering Using Heme Protein Scaffolds To Investigate The Oxidation Of Endogenous Aromatic Amino Acids And Exogenous Substrates
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Author : Thomas Daniel Pfister
language : en
Publisher:
Release Date : 2006
Metalloprotein Engineering Using Heme Protein Scaffolds To Investigate The Oxidation Of Endogenous Aromatic Amino Acids And Exogenous Substrates written by Thomas Daniel Pfister and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.
Protein engineering by rational design has been used to study heme proteins and artificial Mn-salen containing metalloenzymes using heme protein scaffolds. Heme proteins perform a wide variety of functions including the catalysis of numerous different reactions. Some of the structural features involved in conferring these functional properties have been examined in this thesis. Heme protein models have been made of peroxidases and P450 to gain insight into the native function of these enzymes. The role of role of redox active amino acids in heme proteins was studied by mutating Trp an Tyr residues in cytochrome c peroxidase (CcP). Sequential mutation of these residues resulting in increased lifetime of the compound I intermediate. An Engineered Trp in the active site of myoglobin (Mb) was used to mimic bound substrate. The Trp was stoichiometrically hydroxylated to 6-hydroxytryptophan. Factors required to engineer metal binding sites were studied by the redesign of an engineered manganese binding site in CcP. Kinetic parameters and crystal structures of the protein demonstrate the importance of the metal and ligands in engineering metal binding sites. Finally the role of cofactors in catalysis was probed by dually anchoring a Mn-salen complex in the active site of a heme protein scaffold (Mb). Dual anchoring was shown to increase ee and rate in this artificial metalloenzyme. It is concluded that the control of reactive intermediates, presence of appropriate substrate binding sites and the choice of an appropriate scaffold and cofactor are essential in engineering protein models and biocatalysts.
Dissertation Abstracts International
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Author :
language : en
Publisher:
Release Date : 2007
Dissertation Abstracts International written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Dissertations, Academic categories.
2 Oxoglutarate Dependent Oxygenases
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Author : Christopher J Schofield
language : en
Publisher: Royal Society of Chemistry
Release Date : 2015-05-06
2 Oxoglutarate Dependent Oxygenases written by Christopher J Schofield and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-05-06 with Science categories.
Since the discovery of the first examples of 2-oxoglutarate-dependent oxygenase-catalysed reactions in the 1960s, a remarkably broad diversity of alternate reactions and substrates has been revealed, and extensive advances have been achieved in our understanding of the structures and catalytic mechanisms. These enzymes are important agrochemical targets and are being pursued as therapeutic targets for a wide range of diseases including cancer and anemia. This book provides a central source of information that summarizes the key features of the essential group of 2-oxoglutarate-dependent dioxygenases and related enzymes. Given the numerous recent advances and biomedical interest in the field, this book aims to unite the latest research for those already working in the field as well as to provide an introduction for those newly approaching the topic, and for those interested in translating the basic science into medicinal and agricultural benefits. The book begins with four broad chapters that highlight critical aspects, including an overview of possible catalytic reactions, structures and mechanisms. The following seventeen chapters focus on carefully selected topics, each written by leading experts in the area. Readers will find explanations of rapidly evolving research, from the chemistry of isopenicillin N synthase to the oxidation mechanism of 5-methylcytosine in DNA by ten-eleven-translocase oxygenases.
Nanozymes Next Wave Of Artificial Enzymes
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Author : Xiaoyu Wang
language : en
Publisher: Springer
Release Date : 2016-07-27
Nanozymes Next Wave Of Artificial Enzymes written by Xiaoyu Wang and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-07-27 with Technology & Engineering categories.
This book describes the fundamental concepts, the latest developments and the outlook of the field of nanozymes (i.e., the catalytic nanomaterials with enzymatic characteristics). As one of today’s most exciting fields, nanozyme research lies at the interface of chemistry, biology, materials science and nanotechnology. Each of the book’s six chapters explores advances in nanozymes. Following an introduction to the rise of nanozymes research in the course of research on natural enzymes and artificial enzymes in Chapter 1, Chapters 2 through 5 discuss different nanomaterials used to mimic various natural enzymes, from carbon-based and metal-based nanomaterials to metal oxide-based nanomaterials and other nanomaterials. In each of these chapters, the nanomaterials’ enzyme mimetic activities, catalytic mechanisms and key applications are covered. In closing, Chapter 6 addresses the current challenges and outlines further directions for nanozymes. Presenting extensive information on nanozymes and supplemented with a wealth of color illustrations and tables, the book offers an ideal guide for readers from disparate areas, including analytical chemistry, materials science, nanoscience and nanotechnology, biomedical and clinical engineering, environmental science and engineering, green chemistry, and novel catalysis.
Heme Peroxidases
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Author : Emma Raven
language : en
Publisher: Royal Society of Chemistry
Release Date : 2015-10-26
Heme Peroxidases written by Emma Raven and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-10-26 with Science categories.
Heme peroxidases are widely distributed in biological systems and are involved in a wide range of processes essential for life. This book provides a comprehensive single source of information on the various aspects of heme peroxidase structure, function and mechanism of action. Chapters written and edited by worldwide experts span a range of heme peroxidases from plants, yeast, bacteria and mammals. Discussed functions of peroxidases range from cell wall synthesis, synthesis of prostaglandins, role in drug suppression of tuberculosis, and antibacterial activity. Included is a discussion of peroxidases that also act as catalases and oxygenases. Heme Peroxidases serves as an essential text for those working in industry and academia in biochemistry and metallobiology.
Cytochrome P450
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Author : Paul R. Ortiz de Montellano
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-02-05
Cytochrome P450 written by Paul R. Ortiz de Montellano and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-02-05 with Medical categories.
Cytochrome P450: Structure, Mechanism, and Biochemistry, third edition is a revision of a review that summarizes the current state of research in the field of drug metabolism. The emphasis is on structure, mechanism, biochemistry, and regulation. Coverage is interdisciplinary, ranging from bioinorganic chemistry of cytochrome P450 to its relevance in human medicine. Each chapter provides an in-depth review of a given topic, but concentrates on advances of the last 10 years.
Hemoglobin And Myoglobin In Their Reactions With Ligands
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Author : Eraldo Antonini
language : en
Publisher: North-Holland
Release Date : 1971
Hemoglobin And Myoglobin In Their Reactions With Ligands written by Eraldo Antonini and has been published by North-Holland this book supported file pdf, txt, epub, kindle and other format this book has been release on 1971 with Medical categories.
Bioinorganic Photochemistry
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Author : Grazyna Stochel
language : en
Publisher: John Wiley & Sons
Release Date : 2009-06-10
Bioinorganic Photochemistry written by Grazyna Stochel and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-06-10 with Science categories.
Bioinorganic photochemistry is a rapidly evolving field integrating inorganic photochemistry with biological, medical and environmental sciences. The interactions of light with inorganic species in natural systems, and the applications in artificial systems of medical or environmental importance, form the basis of this challenging inter-disciplinary research area. Bioinorganic Photochemistry provides a comprehensive overview of the concepts and reactions fundamental to the field, illustrating important applications in biological, medical and environmental sciences. Topics covered include: Cosmic and environmental photochemistry Photochemistry of biologically relevant nanoassemblies Molecular aspects of photosynthesis Photoinduced electron transfer in biosystems Modern therapeutic strategies in photomedicine The book concludes with an outlook for the future of environmental protection, discussing emerging techniques in the field of pollution abatement, and the potential for bioinorganic photochemistry as a pathway to developing cheap, environmentally friendly sources of energy. Written as an authoritative guide for researchers involved in the development of bioinorganic photochemical processes, Bioinorganic Photochemistry is also accessible to scientists new to the field, and will be a key reference source for advanced courses in inorganic, and bioinorganic chemistry.
Encyclopedia Of Biology
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Author : Don Rittner
language : en
Publisher: Infobase Publishing
Release Date : 2004-08
Encyclopedia Of Biology written by Don Rittner and has been published by Infobase Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-08 with Biology categories.
Contains approximately 800 alphabetical entries, prose essays on important topics, line illustrations, and black-and-white photographs.
Activation Of Small Molecules
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Author : William B. Tolman
language : en
Publisher: John Wiley & Sons
Release Date : 2006-12-13
Activation Of Small Molecules written by William B. Tolman and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-12-13 with Science categories.
The first to combine both the bioinorganic and the organometallic view, this handbook provides all the necessary knowledge in one convenient volume. Alongside a look at CO2 and N2 reduction, the authors discuss O2, NO and N2O binding and reduction, activation of H2 and the oxidation catalysis of O2. Edited by the highly renowned William Tolman, who has won several awards for his research in the field.