Methods And Techniques In Virology
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Methods And Techniques In Virology
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Author : Pierre Payment
language : en
Publisher: CRC Press
Release Date : 1993-07-14
Methods And Techniques In Virology written by Pierre Payment and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-07-14 with Medical categories.
Presents protocols of basic as well as advanced laboratory techniques, and a comprehensive set of investigative practices ranging from the isolation, identification, titration, purification and production of viruses to advanced molecular methods for the analysis of viral proteins and nucleic acids.
Virology Methods Manual
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Author : Hillar O. Kangro
language : en
Publisher: Elsevier
Release Date : 1996-04-16
Virology Methods Manual written by Hillar O. Kangro and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-04-16 with Science categories.
The Virology Methods Manual is a comprehensive source of methods for the study, manipulation, and detection of viruses. Edited by Brian Mahy and Hillar Kangro, this work describes the most up-to-date, definitive techniques, provided by experts in each area, and presented with easy-to-use, step-by-step protocols. This new manual will satisfy the needs of virologists and all those working with viruses who need a practical guide to methods that work! Provides up-to-date techniques by experts worldwide Presents common, step-by-step protocols in an attractive, easy-to-use fashion Contains useful appendices including virus taxonomy, metabolic inhibitors, and Bio-safety in the virology laboratory
Fundamental Techniques In Virology
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Author : Karl Habel
language : en
Publisher:
Release Date : 1969
Fundamental Techniques In Virology written by Karl Habel and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1969 with Science categories.
Tissue culture procedures; Preparation of subcellular fractions; Concentration and purification of viruses; Quantitative assay procedures for virus infectivity; Quantitative procedures for assay of RNA, DNA, and protein; Protein analysis; RNA analyses; Electron microscopic procedures.
Methods In Virology
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Author : Karl Maramorosch
language : en
Publisher: Elsevier
Release Date : 2014-06-28
Methods In Virology written by Karl Maramorosch and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-28 with Science categories.
Methods in Virology, Volume VII focuses on the methods used in virology, including radioimmunoassays, microscopy, hybridization, and mutagenesis. The selection first elaborates on monoclonal antibody techniques applied to viruses; competition radioimmunoassays for characterization of antibody reactions to viral antigens; and enzyme immunosorbent assays in plant virology. Discussions focus on the principles of enzyme immunosorbent assay, choice of enzyme and preparation of conjugate, determination of immunoglobulin class, and maintenance and specificity testing of hybridomas. The text then elaborates on electron microscopy for the identification of plant viruses in in vitro preparations and cloning and expression of viral antigens in Escherichia coli and other microorganisms, including influenza virus, expression of foreign coding sequences in Escherichia coli, hepatitis B virus, electron microscope, immunoelectron microscopy, and imaging of nucleic acids. The manuscript takes a look at the detection and characterization of subgenomic RNA in plant viruses; exploring the gene organization of baculoviruses; and spot hybridization for detection of viroids and viruses. Topics include application to viral diseases, mapping mutuations of baculoviruses, transcriptional mapping of baculovirus genomes, and genetic mapping by blot hybridization. The selection is a valuable source of information for researchers interested in the methods employed in virology.
Methods In Virology
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Author : Karl Maramorosch
language : en
Publisher: Academic Press
Release Date : 2014-06-28
Methods In Virology written by Karl Maramorosch and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-28 with Science categories.
Methods in Virology, Volume III focuses on the advancements of methods employed in virology, including immunological, microscopic, and serological techniques and transformation assays. The selection first offers information on the analysis of protein constituents and lipid components of viruses. Discussions focus on the applications of the existing methodology to lipid-containing viruses; physical methods for the characterization of virus proteins; renaturation of virus proteins and reconstitution of viruses; and chemical methods for the characterization of virus proteins. The text then elaborates on RNA polymerase, immunological techniques for animal viruses, and serological techniques for plant viruses. The book tackles the plaque assay of animal viruses, transformation assays, and the methods for selecting RNA bacteriophage. Topics include identification of the nucleic acid, assay methods for particular viruses, general consideration of the plaque assay method, virus-dilution media and procedures, monolayer assay methods, and incubation and staining of plates and counting of plaques. The manuscript also takes a look at the structural studies of viruses, microscopic techniques, electron microscopy of isolated virus particles and their components, and the application of thin sectioning. The selection is a vital source of data for researchers interested in the methods employed in virology.
Dna Viruses
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Author : Paul M. Lieberman
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Dna Viruses written by Paul M. Lieberman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Medical categories.
A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.
Comprehensive Virology
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Author : Heinz Fraenkel-Conrat
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Comprehensive Virology written by Heinz Fraenkel-Conrat and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The time seems ripe for a critical compendium of that segment of the biological universe we call viruses. Virology, as a science, having passed only recently through its descriptive phase of naming and num bering, has probably reached that stage at which relatively few new truly new-viruses will be discovered. Triggered by the intellectual probes and techniques of molecular biology, genetics, biochemical cytology, and high resolution microscopy and spectroscopy, the field has experienced a genuine information explosion. Few serious attempts have been made to chronicle these events. This comprehensive series, which will comprise some 6000 pages in a total of about 18 volumes, represents a commitment by a large group of active investigators to analyze, digest, and expostulate on the great mass of data relating to viruses, much of which is now amorphous and disjointed, and scattered throughout a wide literature. In this way, we hope to place the entire field in perspective, and to develop an invalua ble reference and sourcebook for researchers and students at all levels. This series is designed as a continuum that can be entered anywhere, but which also provides a logical progression of developing facts and integrated concepts.
Molecular Methods For Virus Detection
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Author : Danny L. Wiedbrauk
language : en
Publisher: Elsevier
Release Date : 1995-02-08
Molecular Methods For Virus Detection written by Danny L. Wiedbrauk and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-02-08 with Science categories.
Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence Includes quality control information crucial in research and clinical laboratories Most chapters are written by the inventors and principal developers of the methodologies Includes color plates, 77 figures, and 18 tables
Methods In Virology
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Author : Karl Maramorosch
language : en
Publisher: Academic Press
Release Date : 2014-06-28
Methods In Virology written by Karl Maramorosch and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-28 with Science categories.
Methods in Virology, Volume V focuses on the methods used in virology, including hybridization, gel electrophoresis, freeze-etching technique, and ultracentrifugation. The selection first offers information on the fusion of cells for virus studies and production of cell hybrids; approaches to ultracentrifugation; and polyacrylamide gel electrophoresis of viral RNA. Discussions focus on applications to virological problems and analysis of results; analysis of the distribution of RNA on polyacrylamide gels after electrophoresis; and biological analysis of DNA components. The book then examines the polyacrylamide gel electrophoresis of viral proteins, DNA-RNA and DNA-DNA hybridization in virus research, and techniques of RNA-DNA hybridization in solution for the study of viral transcription. Topics include preparation of nucleic acids, hybridization and elution procedures that minimize RNA degradation, and procedures for acrylamide gel electrophoresis. The text takes a look at freeze-etching technique for the study of virus ultrastructure; procedures to increase virus yield from infected plants; and the immunoperoxidase technique. Concerns include principles of the immunoperoxidase technique, histochemical detection of peroxidase activity, sequence of events in virus infection, and factors affecting virus yield. The selection is a valuable source of data for researchers interested in the methods employed in virology.
A Manual Of Basic Virological Techniques
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Author : Grace C. Rovozzo
language : en
Publisher: Prentice Hall
Release Date : 1973
A Manual Of Basic Virological Techniques written by Grace C. Rovozzo and has been published by Prentice Hall this book supported file pdf, txt, epub, kindle and other format this book has been release on 1973 with Science categories.