Microprobe Analysis In Medicine
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Microprobe Analysis In Medicine
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Author : Peter Ingram
language : en
Publisher:
Release Date : 1989
Microprobe Analysis In Medicine written by Peter Ingram and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989 with Diagnosis, Electron microscopic categories.
A work reviewing the applications of new microprobe analysis techniques in medicine. Authoritative chapters illustrate a variety of techniques and demonstrate appropriate applications to specific diseases. Key coverage includes pulmonary pathology and asbestoses.
Microprobe Analysis In Biology And Medicine
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Author : Patrick Echlin
language : en
Publisher:
Release Date : 1978
Microprobe Analysis In Biology And Medicine written by Patrick Echlin and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1978 with Biology categories.
Electron Probe Microanalysis
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Author : Karl Zierold
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-08
Electron Probe Microanalysis written by Karl Zierold and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-08 with Science categories.
The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti cular diffusible ions (elements). 2. Electron probe microanaly sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.
Biomedical Applications Of Microprobe Analysis
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Author : Peter Ingram
language : en
Publisher: Academic Press
Release Date : 1999-10-29
Biomedical Applications Of Microprobe Analysis written by Peter Ingram and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-10-29 with Science categories.
Biomedical Applications of Microprobe Analysis is a combination reference/laboratory manual for the use of microprobe analysis in both clinical diagnostic and research settings. Also called microchemical microscopy, microprobe analysis uses high-energy bombardment of cells and tissue, in combination with high resolution EM or confocal microscopy to provide a profile of the ion, metal, and mineral concentrations present in a sample. This allows insight into the physiology and pathophysiology of a wide variety of cells and tissues. This book describes methods for obtaining detailed information about the identity and composition of particles too small to be seen with the naked eye and describes how this information can be useful in diagnostic and biomedical research. Up-to-date review of electron microprobe analysis Detailed descriptions of sample preparation techniques Recent technologies including confocal microscopy, infrared microspectroscopy, and laser raman spectroscopy Over 100 illustrations with numerous specific applications Contributions by world-renowned experts in the field Brief summary of highlights precedes each chapter
Application Of Laser Microprobe Mass Analysis Lamma To Biology And Medicine
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Author : Armand Verbueken
language : en
Publisher:
Release Date : 1985
Application Of Laser Microprobe Mass Analysis Lamma To Biology And Medicine written by Armand Verbueken and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985 with categories.
Electron Microscopy In Human Medicine
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Author : Jan Vincents Johannessen
language : en
Publisher: McGraw-Hill Companies
Release Date : 1983
Electron Microscopy In Human Medicine written by Jan Vincents Johannessen and has been published by McGraw-Hill Companies this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983 with Medical categories.
Applications Of Nuclear Microprobe In The Life Sciences
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Author : Yvan Llabador
language : en
Publisher: World Scientific
Release Date : 1998
Applications Of Nuclear Microprobe In The Life Sciences written by Yvan Llabador and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Science categories.
"... this book will be particularly useful to the many biologists encountering problems of elemental analysis & analytical imaging who wish to have a clear yet comprehensive introduction to the principles, the conditions of application & the potential of nuclear microprobes compared with those of alternative methods." Journal of Trace & Microprobe Techniques, 1999
Quantitative Electron Microprobe Analysis
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Author : Roger Theisen
language : en
Publisher: Springer
Release Date : 1965
Quantitative Electron Microprobe Analysis written by Roger Theisen and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1965 with Science categories.
The Electron "licroprobe X-!{ay Analyscr conceivcd b ' R C.\S'L\I: \G and A. Cl'!: '\ lEI( in 1949 has been developcd as an extremelv po\\'crful tool in spcctrochcmical analysis for a wide range of applications, ranging from qualitative elcmcntary distribution studies, to highly localiscd quantitatin analysis on a one micron scale. \\'ith the increasing number oi' versatile instruments, commcrcially available, the domain of applications - in metallurgy, solid state physics, mineralogy and geology, biology and medicine, arts and archeology - is rapidly expanding, particularly because reliable quantitative analyses can be achieved. It is well established that in multicomponent specimens, the relative x-ray intensity generated by the electron bombardment - i.e. the intensity ratio of the characteristic x-ray radiation emitted under identical experimental conditions by the specimen and a calibration standard - is not directly correlated to the elementary mass concentration. The use of a wide scale of carefully prepared homogeneous calibration standards is generally very tedious and restricted to binar)' systems. For more complex specimens, the conversion of recorded x-ra)' intensity ratios to elementary mass concentration requires, besides carefule selection of experimental conditions, an adequate correction calculation to take account oi' the various physical phenomenas occurring in the tarp;et - electron retardation, electron backseattering, x-ray excitation efficieney, fluorescence enhaneement by eharaeteristic and continuous radiation and x-ray mass absorption.
X Ray Microscopy In Clinical And Experimental Medicine
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Author : Theodore Alvin Hall
language : en
Publisher: Charles C. Thomas Publisher
Release Date : 1972
X Ray Microscopy In Clinical And Experimental Medicine written by Theodore Alvin Hall and has been published by Charles C. Thomas Publisher this book supported file pdf, txt, epub, kindle and other format this book has been release on 1972 with Medical categories.
Microprobe Analysis Of Biological Systems
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Author : Thomas Hutchinson
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Microprobe Analysis Of Biological Systems written by Thomas Hutchinson and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Nature categories.
Microprobe Analysis of Biological Systems covers the proceedings of the 1980 Microprobe Analysis of Biological Systems conference held at Battelle Conference Center in Seattle, Washington. Most of the major laboratories in the field of biological microanalysis in the United States, England, Scotland, France, and Germany are represented. The conference presents the findings, theories, techniques, and procedures of the laboratory represented, no matter how tentative and exploratory. This book is divided into four parts encompassing 22 chapters that focus on biological applications of microprobe analysis. The introductory part describes the application of electron microprobe and X-ray microanalyses in studies of epithelial transport, avian salt gland, electrolyte transport, and acrosome reaction. The subsequent part covers the application of microprobe techniques in the analysis of cardiac, skeletal, vascular smooth, and freeze-dried muscles. It also describes a method for obtaining erythrocyte preparations for validating biological microprobe methods and the continuum-fluorescence effect on thick biological tissue. The method using freeze-substitution to localize calcium in quick-frozen tissue for X-ray microanalysis is also explained. The third part of the book tackles the principles, basic features, and applications of electron energy-loss spectroscopy. Discussions on the use of inner-shell signals for a quantitative local microanalysis technique; theoretical study of the energy resolution; and collection efficiency of a magnetic spectrometer are also included. The final part covers the elemental distribution in single erythrocytes using X-ray microanalysis. It also discusses the fundamentals of cryosectioning process for X-ray microanalysis of diffusible elements and the freezing behavior of a number of chemically different gels chosen for their partial resemblance to biological structures. Considerable chapters contain materials and methods, results, discussions, conclusions, and references. This book will be of value to scientists interested in elemental and ion transport within cells and between cells and extracellular compartments.
