Microtubule Dynamics
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Microtubule Dynamics
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Author : Anne Straube
language : en
Publisher: Humana Press
Release Date : 2017-04-30
Microtubule Dynamics written by Anne Straube and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-04-30 with Science categories.
Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field.
Tubulin And Microtubule Dynamics In Vivo
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Author : William M. Saxton
language : en
Publisher:
Release Date : 1986
Tubulin And Microtubule Dynamics In Vivo written by William M. Saxton and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986 with Microtubules categories.
Microtubule Protocols
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Author : Jun Zhou
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-08-23
Microtubule Protocols written by Jun Zhou and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-23 with Medical categories.
Microtubules are essential components of the cytoskeleton, and play critical roles in a variety of cell processes, including cell shaping, intracellular tracking, cell division, and cell migration. Microtubule Protocols presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs. The straightforward presentation of readily reproducible protocols is a hallmark of the Methods in Molecular MedicineTM series, and is evident in this volume. Methods presented range from the purification and characterization of microtubule proteins, analysis of post-translational modifications of tubulin, and determination of microtubule structure, to the visualization of microtubule and spindle behavior, measurement of microtubule dynamics, and examination of microtubule-mediated cellular processes. Both basic scientists and clinical researchers will benefit from this collection of state-of-the-art protocols for microtubule research.
Microtubule Dynamics In Living Cells
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Author : Paul Joseph Sammak
language : en
Publisher:
Release Date : 1988
Microtubule Dynamics In Living Cells written by Paul Joseph Sammak and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with categories.
Microtubule Dynamics During Mitosis In Living Cells
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Author : Ye Zhai
language : en
Publisher:
Release Date : 1993
Microtubule Dynamics During Mitosis In Living Cells written by Ye Zhai and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with categories.
Microtubule Dynamics And Their Effectors
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Author : Amanda J. Johnson
language : en
Publisher:
Release Date : 1998
Microtubule Dynamics And Their Effectors written by Amanda J. Johnson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with categories.
Being And Becoming The Spindle
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Author : Kenneth E. Sawin
language : en
Publisher:
Release Date : 1993
Being And Becoming The Spindle written by Kenneth E. Sawin and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Microtubules categories.
Microtubules
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Author : Jeremy S. Hyams
language : en
Publisher: Wiley-Liss
Release Date : 1994
Microtubules written by Jeremy S. Hyams and has been published by Wiley-Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.
Microtubules reflects the enormous and significant advances in microtubule research of the past decade. It provides up-to-date information on the art of microtubule research, building on the success of Dr. Hyams's 1979 landmark text. A distinguished group of editors and contributors presents reviews dealing with three central topics: the biochemistry and assembly of the tubulin dimer; associated proteins and regulation of microtubule function; and microtubule organization and function in the cell.
Modelling Microtubule Dynamics And Microtubule Motor Self Organisation
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Author : Jamie Rickman
language : en
Publisher:
Release Date : 2019
Modelling Microtubule Dynamics And Microtubule Motor Self Organisation written by Jamie Rickman and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with categories.
The microtubule cytoskeleton is a self-organising system of microtubule filaments, molecular motors and regulatory proteins that plays a number of vital roles in all eukaryotic cells, acting as a scaffold to provide structural support to the cell and forming tracks for intracellular transport. Arguably one of its most important roles is in assembling the mitotic spindle, the highly dynamic force-generating structure that segregates chromosomes during mitosis. Critical to the spindle's dynamism is the dynamic instability of microtubules. At the heart of the long-standing GTP cap model of dynamic instability is the notion that stochastic fluctuations of the GTP cap drive microtubules to catastrophe, but a quantitative understanding of GTP cap fluctuations is missing. In Chapter 2 a simple mathematical model is developed that predicts microtubule fluctuation behaviour in the steady-state. The theory is validated by data, which suggests that microtubules are remarkably stable during most of their lifetime. How microtubules and motors self-organise into the complex, bipolar architecture of the spindle is not well understood. In Chapter 3 a microscopic computational model of microtubule-motor self-organisation is developed based on in vitro experiments. Two control parameters are identified capturing system composition and kinetics that predict the outcome of self-organisation and uncover generic, mechanistic rules governing active filament-motor networks. Design principles of spindle bipolarity are elucidated which highlight the combined effect of motor activity and filament dynamics on self-organisation. Computer simulations are an increasingly powerful tool for modelling the cytoskeleton, but existing models differ in key respects. In Chapter 4 the effects of spatial dimensionality and the inclusion/omission of steric interactions on simulated microtubule-motor networks is systematically evaluated. Spatial dimensionality and steric interactions are found to have a combined effect on the probability of microtubule crossings, which in turn play critical and distinct roles in several archetypal microtubule-motor networks. Guidelines for appropriate modelling choices are discussed.
Functional Analysis Of Microtubule Dynamics In Saccharomyces Cerevisiae
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Author : Baoying Huang
language : en
Publisher:
Release Date : 2006
Functional Analysis Of Microtubule Dynamics In Saccharomyces Cerevisiae written by Baoying Huang and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.