Molecular Beacons
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Molecular Beacons
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Author : Chaoyong James Yang
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-08-15
Molecular Beacons written by Chaoyong James Yang and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-08-15 with Science categories.
Molecular Beacons explains working principle of molecular beacons, discusses their design, synthesis, purification and characterization, explores their thermodynamic and kinetic properties, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA imaging applications. This book is designed to bring together in a single resource an organized and comprehensive view of molecular beacons and will be a valuable resource for academic, clinical and industrial scientists and graduate students who may consider exploring molecular beacons in their research or practice. Chaoyong James Yang is the Lu Jiaxi Professor of Chemistry at Xiamen University, China. Weihong Tan is a Distinguished Professor of Chemistry and Biomedical Engineering at Hunan University, China and also a University of Florida Distinguished Professor and V. T. and Louis Jackson Professor of Chemistry at the University of Florida, USA.
Molecular Beacons Signalling Nucleic Acid Probes Methods And Protocols
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Author : Andreas Marx
language : en
Publisher: Humana Press
Release Date : 2010-12-10
Molecular Beacons Signalling Nucleic Acid Probes Methods And Protocols written by Andreas Marx and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-10 with Medical categories.
From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals. The book first offers an introduction to the basic principles of fluorescence and then describes applications of fluorogenic probes in real-time PCR, which currently is the gold standard for quantitative DNA and RNA analysis. Coverage extends the potential of realtime as well as advocates simplifications of the probe technologies. It also presents a new simplified molecular beacon design, EasyBeacons, and demonstrates the utility in DNA methylation profiling.
Molecular Beacons For Solution Based Fluorescent In Situ Hybridization Experiments
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Author : Matthew L. Narus
language : en
Publisher:
Release Date : 1999
Molecular Beacons For Solution Based Fluorescent In Situ Hybridization Experiments written by Matthew L. Narus and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with categories.
Progress Toward Synthesis Of Molecular Beacons Incorporating Dabcyl Analog Quenchers
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Author : Christie Ettles
language : en
Publisher:
Release Date : 2013
Progress Toward Synthesis Of Molecular Beacons Incorporating Dabcyl Analog Quenchers written by Christie Ettles and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.
Progress toward the synthesis of molecular beacons containing analogs of the universal quencher 4-(4'-dimethylaminophenylazo)benzoic acid (DABCYL) has been made. A synthetic methodology has been developed for the differential labeling of a PNA molecular beacon with quencher and fluorophore moieties. A DABCYL analog diarylazo compound and three small fluorescent molecules have been derivatized with terminal alkyne groups for incorporation into azide-decorated PNA oligomers by on-resin click chemistry. An optimized on-resin procedure was used to determine the viability of the fluorophores and quencher for incorporation into PNA. The synthesis of a pyrrolocytidine phosphoramidite containing a diarylazo DABCYL analog moiety was undertaken with the aim of incorporation into a DNA molecular beacon by standard phosphoramidite chemistry. While the phosphoramidite was not achieved, an unprecedented addition of the dimethoxytrityl protecting group to the electron-rich pyrrolocytidine heterocycle was observed and a mechanism for its formation is proposed.
Development Of Molecular Beacons For Nucleic Acid Detection
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Author : Salvatore Angelo Efisio Marras
language : en
Publisher:
Release Date : 2003
Development Of Molecular Beacons For Nucleic Acid Detection written by Salvatore Angelo Efisio Marras and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with categories.
Single Molecule Detection Of Molecular Beacons Generated From Ldr On Thermoplastic Microfluidic Device For Bioanalysis
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Author : Zhiyong Peng
language : en
Publisher:
Release Date : 2012
Single Molecule Detection Of Molecular Beacons Generated From Ldr On Thermoplastic Microfluidic Device For Bioanalysis written by Zhiyong Peng and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.
Lifetime Measurements Of Molecular Beacons
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Author : Shannon Dawn Kelley
language : en
Publisher:
Release Date : 2001
Lifetime Measurements Of Molecular Beacons written by Shannon Dawn Kelley and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with categories.
Molecular Beacons
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Author : Timothy J. Drake
language : en
Publisher:
Release Date : 2005
Molecular Beacons written by Timothy J. Drake and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with categories.
Overall, the molecular probes investigated here demonstrated their ability to monitor intracellular mRNA expression; however, future endeavors will include the further improvements in designing the probes and developing a better understanding of their intracellular behaviors.
Molecular Beacons As Rna Probes In Living Cells
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Author : John C. Dalfino
language : en
Publisher:
Release Date : 1997
Molecular Beacons As Rna Probes In Living Cells written by John C. Dalfino and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Nucleic acids categories.
Molecular Beacons For The Detection Of Dna Binding Proteins
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Author : Eric D. Knoll
language : en
Publisher:
Release Date : 2006
Molecular Beacons For The Detection Of Dna Binding Proteins written by Eric D. Knoll and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with DNA-binding proteins categories.