Molecular Force Sensors
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Molecular Force Sensors
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Author : Kerstin Blank
language : en
Publisher:
Release Date : 2006
Molecular Force Sensors written by Kerstin Blank and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.
Molecular Force Sensors
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Author : Rachel Bender
language : en
Publisher: American Chemical Society
Release Date : 2022-03-09
Molecular Force Sensors written by Rachel Bender and has been published by American Chemical Society this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-03-09 with Science categories.
Molecular force sensors are a powerful tool for studying the mechanics of cells. These sensors not only provide information on the general mechanical behavior of cells but also can help elucidate the mechanical markers of disease formation and progression. For example, can we use cell mechanics as a biomarker to diagnose cancer? What are the mechanics associated with immune system function? Can cell mechanics be used to better understand the effect of drug treatments? The number of available questions in the field of mechanobiology is endless. After reading this e-book, you will be equipped with the information needed to begin designing your own molecular force sensors, and to begin answering the multitude of questions surrounding cell mechanobiology.
Dna Based Molecular Force Sensors In Cytoskeletal Networks And Cells
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Author :
language : en
Publisher:
Release Date : 2015
Dna Based Molecular Force Sensors In Cytoskeletal Networks And Cells written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
Eukaryotic cells are highly active and undergo force generating processes such as cell adhesion, migration and division. The cytoskeleton, a polymer meshwork in cells, plays an important role in these cellular processes. Advances in the field of biomechanics have now made it possible to measure the cellular forces in the pN range. We have designed a DNA force sensor, based on FRET, as a tool to investigate multiple cellular processes. The following work encompasses design of the DNA force sensor and validation of its working through control experiments. The sensor was tested in vitro in cyt...
Molecular Force Sensors
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Author : Monique J. Jacobs
language : en
Publisher:
Release Date : 2015
Molecular Force Sensors written by Monique J. Jacobs and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
Coiled Coils As Molecular Force Sensors For The Extracellular Matrix
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Author : Melis Goktas
language : en
Publisher:
Release Date : 2019
Coiled Coils As Molecular Force Sensors For The Extracellular Matrix written by Melis Goktas and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with categories.
Force plays a fundamental role in the regulation of biological processes. Cells can sense the mechanical properties of the extracellular matrix (ECM) by applying forces and transmitting mechanical signals. They further use mechanical information for regulating a wide range of cellular functions, including adhesion, migration, proliferation, as well as differentiation and apoptosis. Even though it is well understood that mechanical signals play a crucial role in directing cell fate, surprisingly little is known about the range of forces that define cell-ECM interactions at the molecular level. ...
Feeling The Force
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Author : Hari Shroff
language : en
Publisher:
Release Date : 2006
Feeling The Force written by Hari Shroff and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.
Molecular Fluorescent Reporters For Force And Smart Surfaces For Sensing Cell Surface Interaction
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Author : Mariya Barch
language : en
Publisher:
Release Date : 2009
Molecular Fluorescent Reporters For Force And Smart Surfaces For Sensing Cell Surface Interaction written by Mariya Barch and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with categories.
Molecular sensors are powerful because they make it possible to adapt the measurement to the sample instead of a sample to an instrument. Many reporter are available for measuring the chemical properties of a sample, but no purpose-built molecular sensors exist to report a sample's mechanical properties. To address recent interest in the mechanical coordinate of molecular interactions, we developed a prototype molecular sensor, calibrated its force-fluorescence relationship, and adapted the sensor to a cell adhesion assay. This thesis focuses on the considerations for combining force measurement with the environmental and distance sensitivity offered by fluorescence to measure cell-surface adhesion. We showed that DNA can be used as a scaffold to build a sensor molecule, that fluorescence can be used as a reporter of a threshold force, and that introducing cells to the sensor molecules changes the fluorescence properties. Because Cy3 experiences an enhanced intensity sensitivity when conjugated to DNA, the reporter's FRET signal was occluded and we instead activated the sensor complex as a novel, all-fluorescent means of reporting cell-surface proximity. This method for reporting cell-surface separation is significant because it simplifies measurements in thicker and more complex materials interesting to cell-substrate interaction studies.
Novel Sensors Technologies Applied To Force Spectroscopy In Molecular Biology
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Author :
language : en
Publisher:
Release Date : 2015
Novel Sensors Technologies Applied To Force Spectroscopy In Molecular Biology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
Force plays an essential role in all fields of biology. Measurement of these forces with high precision provides information about the structure, dynamics, intra and intermolecular interactions, and the mechanical properties of the biomolecules and, in general, about molecular basis of diverse biological phenomena. Different techniques have been developed to address this task, particularly at the single molecule level. The main objective accomplished in this work of thesis is the development of sensors technologies applied to force spectroscopy measurements and the demonstration of its possibilities in real molecular studies. Scanning probe microscopy (SPM) is a fast growing technology that has been the source for the development of an immense variety of applications to investigate materials and molecules at nanoscale. As another technology, SPM is constantly improving through different advances in instrumentation level and the emergence of new applications. From the analysis of the main limitations of quartz tuning fork (QTF) based nanosensors on one side and the conventional force spectroscopy with cantilever tips on the other side, the main considerations have been determined for the technological developments on force microscopy applications. One of the main limitations of tuning fork probes is that they are usually custom-made because no commercial probes suitable for a wide range of experiments are available. The custom-made devices show considerable variation in dynamic response, poor lateral resolution and the characterization of the sensors remains unclear for force quantification. A new controller is developed to ensure the same dynamic response of different sensors in order to maintain the conditions in which the measurements are conducted. Also, a new method to improve lateral resolution of the QTF probes when working in liquid is proposed in this thesis based on attaching a standard AFM tip to the end of the fiber probe which has been previously sharpened. A method to calculate the spring constant of the QTF based sensors from easily measurable parameters is presented in this thesis. The method is based on a finite element analysis (FEA) model which includes the electrical part and can be used to calculate the spring constant of a QTF accurately for quantitative measurements. Results obtained in real biological experiments are promising and show the possibilities of the shear force microscopy improvements developed in this work of thesis. In a first experiment, a self-assembled monolayer (SAM) of micropatterned antibodies was imaged with three different techniques and in a second experiment, a molecular interaction analysis was done between biotin- streptavidin complex and results are compared with those obtained with AFM tip. The main problem for comparing steered molecular dynamics (SMD) simulation results with experimental data is that SMD simulations were restricted to nanosecond timescales (due to the high computational demand of all-atomistic simulations). A high-speed force spectroscopy methodology has been developed to achieve rates comparable to SMD simulations. The validation of the technique is performed with titin unfolding measurements allowing the direct comparison of experimental and simulated forces.
Nanoscale Force Sensors To Study Supramolecular Systems
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Author :
language : en
Publisher:
Release Date : 2016
Nanoscale Force Sensors To Study Supramolecular Systems written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with categories.
Molecular Sensors And Nanodevices
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Author : John X. J. Zhang
language : en
Publisher: Academic Press
Release Date : 2018-11-19
Molecular Sensors And Nanodevices written by John X. J. Zhang and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-11-19 with Technology & Engineering categories.
Molecular Sensors and Nanodevices: Principles, Designs and Applications in Biomedical Engineering, Second Edition is designed to be used as a foundational text, aimed at graduates, advanced undergraduates, early-career engineers and clinicians. The book presents the essential principles of molecular sensors, including theories, fabrication techniques and reviews. In addition, important devices and recently, highly-cited research outcomes are also cited. This differentiates the book from other titles on the market whose primary focus is more research-oriented and aimed at more of a niche market. Covers the fundamental principles of device engineering and molecular sensing, sensor theories and applications in biomedical science and engineering Introduces nano/micro fabrication techniques, including MEMS, bioMEMS, microTAS and nanomaterials science that are essential in the miniaturization of versatile molecular sensors Explores applications of nanomaterials and biomaterials, including proteins, DNAs, nanoparticles, quantum dots, nanotubes/wires and graphene in biomedicine